{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:23:53Z","timestamp":1792002233076,"version":"4.4.0"},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,3]]},"abstract":"<jats:p>We compared the diagnostic performance of the standard method (Kato-Katz) with two recently developed methods (Mini-FLOTAC and Flukefinder) for the detection and quantification of <jats:italic>Fasciola hepatica<\/jats:italic> eggs in human stool. Uninfected human stool samples were artificially spiked with <jats:italic>F. hepatica<\/jats:italic> eggs to reach final concentrations of 14, 28, 41, or 96 eggs per gram of stool (epg). Only Flukefinder showed 100% sensitivity in all but the samples with the lowest concentration of eggs (14 epg), in which it had a sensitivity of 60%. Each of the methods underestimated the true fecal egg counts (FECs), Flukefinder resulting in the most biased egg counts (egg counts 0.18 times lower than the expected FECs). Only the Flukefinder resulted in more precise results (coefficient of variance &lt; 30%) from FECs of 96 epg onward. The outcome of this study indicates that the Flukefinder is a useful alternative diagnostic method for human fascioliasis in stool.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0988","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T07:00:49Z","timestamp":1557212449000},"page":"59-61","source":"Crossref","is-referenced-by-count":36,"title":["Comparison of Kato-Katz Thick Smear, Mini-FLOTAC, and Flukefinder for the Detection and Quantification of Fasciola hepatica Eggs in Artificially Spiked Human Stool"],"prefix":"10.4269","volume":"101","author":[{"given":"Daniel A.","family":"Z\u00e1rate-Rend\u00f3n","sequence":"first","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Facultad de Zootecnia, Universiad Nacional Agraria La Molina, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnny","family":"Vlaminck","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Levecke","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Briones-Montero","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Facultad de Zootecnia, Universiad Nacional Agraria La Molina, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Geldhof","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology, Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Parasite Immunol","first-page":"558","article-title":"A prospective view of animal and human 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study","volume":"100","author":"Marcos","year":"2006"},{"key":"b6","series-title":"Rev Gastroenterol Peru","first-page":"389","article-title":"Fasciola hepatica infection in Peru: an emergent disease","volume":"27","author":"Marcos","year":"2007"},{"key":"b7","series-title":"PLoS Negl Trop Dis","first-page":"e0004361","article-title":"Treatment failure after multiple courses of triclabendazole among patients with fascioliasis in Cusco, Peru: a case series","volume":"10","author":"Cabada","year":"2016"},{"key":"b8","series-title":"Rev Peru Med Exp Salud Publica","first-page":"604","article-title":"Human and animal fascioliasis in Peru: impact in the economy of endemic zones","volume":"27","author":"Espinoza","year":"2010"},{"key":"b9","series-title":"Rev Gastroenterol Peru","first-page":"31","article-title":"Hyperendemic and mesoendemic zones of Fasciola infection surrounding urban Lima: an emerging disease?","volume":"27","author":"Marcos","year":"2007"},{"key":"b10","series-title":"Rev Gastroenterol Peru","first-page":"228","article-title":"Clinical characteristics of chronic infection by Fasciola hepatica in children","volume":"22","author":"Marcos Raymundo","year":"2002"},{"key":"b11","series-title":"Am J Trop Med Hyg","first-page":"481","article-title":"Burden of Fasciola hepatica infection among children from Paucartambo in Cusco, Peru","volume":"86","author":"Lopez","year":"2012"},{"key":"b12","series-title":"Am J Trop Med Hyg","first-page":"989","article-title":"Fascioliasis and eosinophilia in the highlands of Cuzco, Peru and their association with water and socioeconomic factors","volume":"91","author":"Cabada","year":"2014"},{"key":"b13","series-title":"Acta Trop","first-page":"119","article-title":"Hyperendemic human fascioliasis in Andean valleys: an altitudinal transect analysis in children of Cajamarca province, Peru","volume":"120","author":"Gonzalez","year":"2011"},{"key":"b14","series-title":"Parasitology","first-page":"1918","article-title":"Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario","volume":"141","author":"Mas-Coma","year":"2014"},{"key":"b15","series-title":"Nat Protoc","first-page":"1723","article-title":"The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals","volume":"12","author":"Cringoli","year":"2017"},{"key":"b16","series-title":"Geospat Health","first-page":"S67","article-title":"A one-health integrated approach to control fascioliasis in the Cajamarca valley of Peru","volume":"6","author":"Rinaldi","year":"2012"},{"key":"b17","series-title":"Arq Bras Med Vet Zootec","first-page":"1023","article-title":"Concord\u00e2ncia entre duas t\u00e9cnicas coproparasitol\u00f3gicas para diagn\u00f3stico de Fasciola hepatica em 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