{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T16:48:01Z","timestamp":1780764481036,"version":"3.54.1"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,7]]},"abstract":"<jats:p>The conventional method of detecting <jats:italic>Strongyloides stercoralis<\/jats:italic> in fecal samples has poor diagnostic sensitivity. Detection of <jats:italic>Strongyloides<\/jats:italic>-specific antibodies increases the sensitivity; however, most tests are ELISAs that use parasite extract which may cross-react with the sera of other helminth infections. To improve the serological diagnosis of strongyloidiasis, this study aimed at developing a sensitive and specific lateral flow rapid dipstick test. Two recombinant proteins, recombinant NIE (rNIE) and recombinant Ss1a (rSs1a), were used in preparing the dipstick, with gold-conjugated antihuman IgG4 as detector reagent. In parallel, the corresponding ELISA was performed. Both assays demonstrated diagnostic sensitivity of 91.3% (21\/23) when tested with serum samples of patients with <jats:italic>Strongyloides<\/jats:italic> infection, and 100% specificity with 82 sera of asymptomatic (healthy) and those with other parasitic infections. The ELISA and dipstick test results were positively correlated to each other (<jats:italic>r<\/jats:italic> = 0.6114, <jats:italic>P<\/jats:italic> = 0.0019). The developed lateral flow dipstick test may improve the serodiagnosis of strongyloidiasis and merit further validation studies.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0053","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T07:00:30Z","timestamp":1560841230000},"page":"432-435","source":"Crossref","is-referenced-by-count":42,"title":["Lateral Flow Dipstick Test for Serodiagnosis of Strongyloidiasis"],"prefix":"10.4269","volume":"101","author":[{"given":"Muhammad Hafiznur","family":"Yunus","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norsyahida","family":"Arifin","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Balachandra","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nor Suhada","family":"Anuar","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahmah","family":"Noordin","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"PLoS Negl Trop Dis","first-page":"e2288","article-title":"Strongyloides stercoralis: global distribution and risk 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diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology","volume":"8","author":"Pak","year":"2014"},{"key":"b19","series-title":"Nat Methods","first-page":"671","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012"},{"issue":"18","key":"b20","series-title":"J Microbiol Immunol Infect pii:","first-page":"30426","article-title":"Serodiagnosis and early detection of Strongyloides stercoralis infection","volume":"S1684\u20131182","author":"Arifin","year":"2018"},{"key":"b21","series-title":"Trans R Soc Trop Med Hyg","first-page":"609","article-title":"Humoral responses in human strongyloidiasis: correlations with infection chronicity","volume":"91","author":"Atkins","year":"1997"},{"key":"b22","series-title":"World J Microbiol Biotechnol","first-page":"1077","article-title":"Evaluation of the cocktail recombinant antigens, LipL32 and LipL41 for serodiagnosis of canine leptospirosis","volume":"27","author":"Subathra","year":"2011"},{"key":"b23","series-title":"Am J Trop Med Hyg","first-page":"32","article-title":"A lateral flow rapid test for human toxocariasis developed using three Toxocara canis recombinant antigens","volume":"98","author":"Yunus","year":"2018"}],"container-title":["The American Journal of Tropical Medicine and 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