{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T21:39:28Z","timestamp":1776461968275,"version":"3.51.2"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,3]]},"abstract":"<jats:p><jats:italic>Cryptosporidium<\/jats:italic> is a parasite that causes watery diarrhea among both children and adults. However, because many physicians do not routinely ask for <jats:italic>Cryptosporidium<\/jats:italic> diagnostic test, cryptosporidiosis prevalence is likely underestimated. The current study investigated the prevalence of cryptosporidiosis among children admitted to the hospital with gastrointestinal symptoms. Stool sample was collected from each child and subjected to routine microbiological culture<jats:italic>. Cryptosporidium<\/jats:italic> presence was tested by three different methods: real-time PCR (RT-PCR), quick antigen, and microscopic examination with acid-fast staining. Each positive specimen was further tested with BioFire FilmArray Multiplex PCR (bioM\u00e9rieux SA, Marcy-l'Etoile, France) to determine that <jats:italic>Cryptosporidium<\/jats:italic> is the only pathogen in the sample. Demographic and epidemiological data were collected from the patients\u2019 medical records. Of 291 patient stool samples, nine were positive for <jats:italic>Cryptosporidium hominis<\/jats:italic> or <jats:italic>Cryptosporidium parvum.<\/jats:italic> The average age of the nine <jats:italic>Cryptosporidium<\/jats:italic>-positive cases was 2.3 years, lower than the average age of the study population (4.2 years). Of the positive cases, 66.7% were Arabs and 33.3% were Jews. The main complaint of children with <jats:italic>Cryptosporidium<\/jats:italic> was diarrhea. Regarding the laboratory methods for <jats:italic>Cryptosporidium<\/jats:italic> identification, of the positive samples, 100% (9\/9) were identified by RT-PCR, 88% (8\/9) were positive by antigen test, and only 67% (6\/9) were positive by microscopic examination with acid-fast staining. Because of the low incidence of <jats:italic>Cryptosporidium<\/jats:italic> among patients in our facility, the prevalence of cryptosporidiosis still cannot be established. Nevertheless, among the other pathogens found in stool samples, <jats:italic>Cryptosporidium<\/jats:italic> is the second leading cause of hospitalization due to gastrointestinal disease in children in our area.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0057","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T07:01:10Z","timestamp":1558422070000},"page":"160-163","source":"Crossref","is-referenced-by-count":25,"title":["The Prevalence of Cryptosporidium among Children Hospitalized because of Gastrointestinal Symptoms and the Efficiency of Diagnostic Methods for Cryptosporidium"],"prefix":"10.4269","volume":"101","author":[{"given":"Efrat","family":"Golan Shaposhnik","sequence":"first","affiliation":[{"name":"The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Said","family":"Abozaid","sequence":"additional","affiliation":[{"name":"The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel;"},{"name":"Department of Pediatrics, Baruch Padeh Medical Center, Poriya, Israel;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamar","family":"Grossman","sequence":"additional","affiliation":[{"name":"Reference Parasitology Laboratory, Central Laboratories, Ministry of Health, Jerusalem, Israel;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Marva","sequence":"additional","affiliation":[{"name":"Reference Parasitology Laboratory, Central Laboratories, Ministry of Health, Jerusalem, Israel;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avi","family":"On","sequence":"additional","affiliation":[{"name":"The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel;"},{"name":"Pediatric Gastrointestinal Unit, Baruch Padeh Medical Center, Poriya, Israel;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Azrad","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avi","family":"Peretz","sequence":"additional","affiliation":[{"name":"The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel;"},{"name":"Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Vet Parasitol","first-page":"37","article-title":"Cryptosporidium: a water-borne zoonotic parasite","volume":"126","author":"Fayer","year":"2004"},{"key":"b2","series-title":"Clin Microbiol Rev","first-page":"145","article-title":"Epidemiology and clinical features of Cryptosporidium infection in immunocompromised 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Epidemiology and clinical aspects [article in Hebrew]","volume":"118","author":"Miron","year":"1990"},{"key":"b21","series-title":"Clin Microbiol Rev","first-page":"72","article-title":"Cryptosporidium taxonomy: recent advances and implications for public health","volume":"17","author":"Xiao","year":"2004"},{"key":"b22","series-title":"Isr J Med Sci","first-page":"380","article-title":"Cryptosporidiosis in Bedouin and Jewish infants and children in southern Israel","volume":"27","author":"Dagan","year":"1991"},{"key":"b23","series-title":"J Clin Microbiol","first-page":"623","article-title":"Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens","volume":"41","author":"Johnston","year":"2003"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/101\/1\/article-p160.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/101\/1\/article-p160.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/101\/1\/article-p160.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T21:17:47Z","timestamp":1722460667000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/101\/1\/article-p160.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,3]]},"references-count":23,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.19-0057","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,3]]}}}