{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T11:11:11Z","timestamp":1769166671397,"version":"3.49.0"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,3]]},"abstract":"<jats:p>Japanese encephalitis virus (JEV) is the most common cause of viral encephalitis in Asia, and it is increasingly a global public health concern because of its recent geographic expansion. Although commercial vaccines are available and used in some endemic countries, JEV continues to cause illness, with more than 60,000 cases reported annually. To develop a reproducible positive control antibody useable in diagnosis of JEV infections, murine hybridomas were developed from mice inoculated with a combination of IXIARO JEV vaccine and JEV domain III of the envelope protein (E-DIII). Monoclonal antibodies (MAbs) were characterized for their ability to neutralize virus in vitro. Monoclonal antibody 17BD3-2 was found to be JEV specific and highly neutralizing, with a plaque reduction neutralization test (PRNT)<jats:sub>90<\/jats:sub> endpoint titer of 1.25 \u03bcg\/mL. The functional epitopes were mapped using virus neutralization escape variants to amino acid residues S309, K312, and G333 in E-DIII. This MAb may be substituted for human immune sera used as a positive control in PRNT for distribution to public health laboratories worldwide in potential future outbreaks of JEV.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0073","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T07:01:03Z","timestamp":1558422063000},"page":"233-236","source":"Crossref","is-referenced-by-count":9,"title":["A Monoclonal Antibody Specific for Japanese Encephalitis Virus with High Neutralizing Capability for Inclusion as a Positive Control in Diagnostic Neutralization Tests"],"prefix":"10.4269","volume":"101","author":[{"given":"Amanda E.","family":"Calvert","sequence":"first","affiliation":[{"name":"Arboviral Diseases Branch, Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan L.","family":"Bennett","sequence":"additional","affiliation":[{"name":"Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kandice L.","family":"Dixon","sequence":"additional","affiliation":[{"name":"Arboviral Diseases Branch, Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol D.","family":"Blair","sequence":"additional","affiliation":[{"name":"Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John T.","family":"Roehrig","sequence":"additional","affiliation":[{"name":"Arboviral Diseases Branch, Division of Vector-Borne Diseases, U.S. Centers for Disease Control and Prevention, Fort Collins, Colorado;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Zoonoses Public Health","first-page":"338","article-title":"The zoonotic flaviviruses of southern, south-eastern and eastern Asia, and Australasia: the potential for emergent viruses","volume":"56","author":"Mackenzie","year":"2009"},{"key":"b2","series-title":"Annu Rev Entomol","first-page":"17","article-title":"Ecology and geographical expansion of Japanese encephalitis virus","volume":"54","author":"van den Hurk","year":"2009"},{"key":"b3","series-title":"Wkly Epidemiol Rec","first-page":"69","article-title":"Japanese encephalitis vaccines: WHO position paper \u2013 February 2015","volume":"90","author":"WHO","year":"2015"},{"key":"b4","series-title":"J Virol","first-page":"1994","article-title":"Immunodominance and functional activities of antibody responses to inactivated West Nile virus 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