{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:02:17Z","timestamp":1780452137747,"version":"3.54.1"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,2]]},"abstract":"<jats:p><jats:italic>Strongyloides stercoralis<\/jats:italic> and human T-lymphotropic virus 1 (HTLV-1) coinfections have been extensively reported in the literature, but the diagnosis and treatment of strongyloidiasis remains a challenge, particularly in HTLV-1 carriers. Our objectives were to evaluate the efficacy of a new PCR method for the detection of <jats:italic>S. stercoralis<\/jats:italic> in HTLV-1\u2013positive patients. Stools were collected over a 1-year period across the endemic region of French Guiana, including remote forest areas. Two systems of real-time PCR were then used comparatively, with small subunit and specific repeat as respective targets, and compared with the results of microscopic examinations. One-hundred and twelve stool samples were included. Twenty-seven patients (24.1%) presented a positive HTLV-1 serology. The overall prevalence of strongyloidiasis among the 112 patients was 30% with small-subunit PCR and 11.6% with microscopic examinations. In the seropositive population, all tested stools were negative, whereas 51.2% were positive using small-subunit PCR. Thus, PCR allowed a much-improved sensitivity, particularly in HTLV-1 carriers. Among the two systems investigated, small subunit yielded better results than specific repeat PCR, with prevalence rates in HTLV-1 carriers of 51.2% and 22.2%, respectively. Therefore, PCR should be considered as a useful tool for the diagnosis of strongyloidiasis, particularly in HTLV-1 carriers who often present a light parasitic load due to erratic administration of anthelmintic drugs.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0082","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T07:00:30Z","timestamp":1566284430000},"page":"848-850","source":"Crossref","is-referenced-by-count":7,"title":["Utility of PCR in Patients with Strongyloides stercoralis and HTLV-1 Coinfection in French Guiana"],"prefix":"10.4269","volume":"101","author":[{"given":"Romain","family":"Blaizot","sequence":"first","affiliation":[{"name":"Service de Dermatologie, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"},{"name":"EA 3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, Universit\u00e9 de Guyane, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Simon","sequence":"additional","affiliation":[{"name":"EA 3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, Universit\u00e9 de Guyane, Cayenne, French Guiana;"},{"name":"Centre Hospitalier Andr\u00e9e Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Brottier","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Andr\u00e9e Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Blanchet","sequence":"additional","affiliation":[{"name":"EA 3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, Universit\u00e9 de Guyane, Cayenne, French Guiana;"},{"name":"Centre Hospitalier Andr\u00e9e Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Brousse","sequence":"additional","affiliation":[{"name":"Centres D\u00e9localis\u00e9s de Pr\u00e9vention et de Soins, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachida","family":"Boukhari","sequence":"additional","affiliation":[{"name":"Laboratoire, Centre Hospitalier de l\u2019Ouest Guyanais, Saint-Laurent-Du-Maroni, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magalie","family":"Demar","sequence":"additional","affiliation":[{"name":"EA 3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, Universit\u00e9 de Guyane, Cayenne, French Guiana;"},{"name":"Centre Hospitalier Andr\u00e9e Rosemon, Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Cayenne, French Guiana;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Parasite","first-page":"167","article-title":"Intestinal parasitoses among Wayampi Indians from French Guiana","volume":"9","author":"Carme","year":"2002"},{"key":"b2","series-title":"Lancet","first-page":"633","article-title":"High incidence of HTLV antibody in carriers of Strongyloides stercoralis","volume":"1","author":"Nakada","year":"1984"},{"key":"b3","series-title":"Am J Trop Med Hyg","first-page":"246","article-title":"Impairment of host immune response against Strongyloides stercoralis by human T cell lymphotropic virus type 1 infection","volume":"74","author":"Hirata","year":"2006"},{"key":"b4","series-title":"Clin Exp Immunol","first-page":"354","article-title":"Reduced efficacy of treatment of strongyloidiasis in HTLV-I carriers related to enhanced expression of IFN-gamma and 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