{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T12:00:15Z","timestamp":1742385615529},"reference-count":36,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,6]]},"abstract":"<jats:p>Strongyloidiasis, caused by <jats:italic>Strongyloides stercoralis<\/jats:italic> infection, is an important neglected tropical disease that causes significant public health problems in the tropics and subtropics. The disease can persist in hosts for decades and may be life-threatening because of hyperinfection and dissemination. Ivermectin (mostly) and albendazole are the most common anthelmintics used for treatment. Albendazole is suboptimal for this parasite, and although ivermectin is quite effective in immunocompromised patients, a multiple-course regimen is required. Furthermore, reliance on a single drug class for treating intestinal nematodes is a recipe for future failure. Therefore, it is important to discover new anthelmintics to treat or prevent human strongyloidiasis. One promising candidate is the <jats:italic>Bacillus thuringiensis<\/jats:italic> crystal protein Cry5B. Cry5B is highly potent against parasitic nematodes, for example, hookworms and <jats:italic>Ascaris suum<\/jats:italic>. Here, we investigated the potential of Cry5B against <jats:italic>S. stercoralis<\/jats:italic>. Multiple stages of <jats:italic>S. stercoralis<\/jats:italic>, including the first larval stage (L1s), infective stage (iL3s), free-living adult stage, and parasitic female stage, were all susceptible to Cry5B as indicated by impairment of motility and decreased viability in vitro. In summary, Cry5B demonstrated strong potential as an effective anthelmintic for treatment and transmission control of human strongyloidiasis, justifying further experiments to investigate in vivo therapeutic efficacy.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0083","type":"journal-article","created":{"date-parts":[[2019,9,13]],"date-time":"2019-09-13T07:00:27Z","timestamp":1568358027000},"page":"1177-1182","source":"Crossref","is-referenced-by-count":8,"title":["Bacillus thuringiensis Cry5B is Active against Strongyloides stercoralis in vitro"],"prefix":"10.4269","volume":"101","author":[{"given":"Sarit","family":"Charuchaibovorn","sequence":"first","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"},{"name":"Siriraj Center of Excellence for Stem Cell Research (SiSCR), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivornpun","family":"Sanprasert","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataya","family":"Sutthanont","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Hu","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts;"},{"name":"Biology Department, Worcester State University, Worcester, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ambily","family":"Abraham","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Ostroff","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffi V.","family":"Aroian","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tegegn G.","family":"Jaleta","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James B.","family":"Lok","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surang","family":"Nuchprayoon","sequence":"additional","affiliation":[{"name":"Lymphatic Filariasis and Tropical Medicine Research Unit, Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"BMJ","first-page":"f4610","article-title":"Strongyloides stercoralis infection","volume":"347","author":"Greaves","year":"2013"},{"key":"b2","series-title":"Int 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