{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T20:33:20Z","timestamp":1779309200585,"version":"3.51.4"},"reference-count":53,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,3]]},"abstract":"<jats:p>Cutaneous leishmaniasis (CL) remains one of the world\u2019s most prevalent neglected diseases, particularly in developing countries. Identification of the involved <jats:italic>Leishmania<\/jats:italic> species is an important step in the diagnosis and case management process. In this study, we tested simple, rapid, and highly sensitive loop-mediated isothermal amplification (LAMP) assays for <jats:italic>Leishmania<\/jats:italic> DNA species-specific detection from cutaneous lesions. Two LAMP assays, targeting cysteine protease B (cpb) gene, were developed to detect and identify <jats:italic>Leishmania major<\/jats:italic> and <jats:italic>Leishmania tropica<\/jats:italic> species. Loop-mediated isothermal amplification specificity was examined using DNA samples from other <jats:italic>Leishmania<\/jats:italic> species and <jats:italic>Trypanosoma<\/jats:italic> species. No cross-reactions were detected. The developed LAMP assays exhibited sensitivity with a detection limit of 20 fg and 200 fg for <jats:italic>L. major<\/jats:italic> and <jats:italic>L. tropica<\/jats:italic>, respectively. Both tests were applied on clinical samples of CL suspected patients living in endemic Tunisian regions and compared with kinetoplast DNA quantitative PCR (qPCR), microscopic, and conventional cpb-based polymerase chain reaction (PCR) assays. Our LAMP tests were able to discriminate between <jats:italic>L. major<\/jats:italic> and <jats:italic>L. tropica<\/jats:italic> species and showed a sensitivity of 84% and a specificity of 100%. However, when compared with the performance of the diagnostic tests with latent class analysis (LCA), our LAMP assays show a sensitivity of 100%. These assays can be used as a first-line molecular test for early diagnosis and prompt management of CL cases in public health programs.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0097","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T07:01:46Z","timestamp":1557817306000},"page":"101-107","source":"Crossref","is-referenced-by-count":13,"title":["Development and Assessment of Leishmania major and Leishmania tropica Specific Loop-Mediated Isothermal Amplification Assays for the Diagnosis of Cutaneous Leishmaniasis in Tunisia"],"prefix":"10.4269","volume":"101","author":[{"given":"Melek","family":"Chaouch","sequence":"first","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"},{"name":"Laboratory of Bioinformatics, Biomathematics and Biostatistics LR 16 IPT 09, Institut Pasteur de Tunis, Tunis, Tunisia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"},{"name":"Laboratory of Parasitology and Mycology, Institut Pasteur de Tunis, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souad","family":"Ben Othman","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meriem","family":"Ben Abid","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Ben Sghaier","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aida","family":"Bouratbine","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"},{"name":"Laboratory of Parasitology and Mycology, Institut Pasteur de Tunis, Tunis, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Souha","family":"Ben Abderrazak","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Parasitology, Biotechnology and Biomolecules LR 11 IPT 06, Institut Pasteur de Tunis, Tunis, Tunisia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"PLoS One","first-page":"e35671","article-title":"Leishmaniasis worldwide and 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