{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T12:06:02Z","timestamp":1781611562341,"version":"3.54.5"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,2]]},"abstract":"<jats:p>Angiostrongyliasis is a foodborne disease caused by a zoonotic nematode, <jats:italic>Angiostrongylus cantonensis<\/jats:italic>, which produces eosinophilic meningitis or meningoencephalitis (EOM) in humans. Definitive diagnosis is rarely possible because worms are almost never recovered from patients. Human disease can be diagnosed by clinical symptoms and serological tests. Presently, diagnosis is performed by serological detection of antibodies against specific somatic antigens (molecular mass 29\u201331 kDa) extracted from female worms. The life cycle of <jats:italic>A. cantonensis<\/jats:italic> must be maintained in the laboratory to provide a source of this diagnostic antigen. Here, we cloned and expressed recombinant <jats:italic>A. cantonensis<\/jats:italic> galectin-2 (rAcGal2) corresponding to a 31-kDa antigenic peptide. Recombinant protein was purified and used in immunoblot tests, which showed reactions with human serum panels consisting of six confirmed angiostrongyliasis and 24 clinically diagnosed cases of EOM-associated with angiostrongyliasis, 160 samples from patients with other parasitic infections, and 30 samples from normal healthy subjects. Accuracy, sensitivity, specificity, and positive and negative predictive values were 95.0%, 93.3%, 95.3%, 75.7%, and 98.9%, respectively. The test was nonreactive with sera of human gnathostomiasis and cysticercosis, two diseases that could present similar neurological symptoms. Recombinant AcGal2 has potential as a diagnostic antigen and could replace native parasite antigens in further development of an angiostrongyliasis serodiagnostic test kit.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0208","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T07:00:23Z","timestamp":1565074823000},"page":"851-858","source":"Crossref","is-referenced-by-count":6,"title":["Application of Recombinant Angiostrongylus cantonensis Galectin-2 Protein for Serodiagnosis of Human Angiostrongyliasis by Immunoblotting"],"prefix":"10.4269","volume":"101","author":[{"given":"Chalermchai","family":"Somboonpatarakun","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaisiri","family":"Wongkham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Southeast Asian J Trop Med Public Health","first-page":"161","article-title":"Clinical manifestations and laboratory diagnosis of eosinophilic meningitis syndrome associated with angiostrongyliasis","volume":"9","author":"Cross","year":"1978"},{"key":"b2","series-title":"Int J Infect Dis","first-page":"161","article-title":"Angiostrongylus cantonensis eosinophilic meningitis","volume":"3","author":"Pien","year":"1999"},{"key":"b3","series-title":"Am J Med","first-page":"109","article-title":"Eosinophilic meningitis caused by Angiostrongylus cantonensis: report of 17 cases","volume":"111","author":"Tsai","year":"2001"},{"key":"b4","series-title":"Lancet Infect Dis","first-page":"621","article-title":"Human angiostrongyliasis","volume":"8","author":"Wang","year":"2008"},{"key":"b5","series-title":"Hawaii J Med Public Health","first-page":"15","article-title":"Genetic diversity of the rat lungworm, Angiostrongylus cantonensis, the major cause of eosinophilic meningitis","volume":"72","author":"Eamsobhana","year":"2013"},{"key":"b6","series-title":"Hawaii J Med Public Health","first-page":"18","article-title":"Angiostrongylus cantonensis and rat lungworm disease in Brazil","volume":"72","author":"Thiengo","year":"2013"},{"key":"b7","series-title":"Hawaii J Med Public Health","first-page":"26","article-title":"Human parasitic meningitis caused by Angiostrongylus cantonensis infection in Taiwan","volume":"72","author":"Tsai","year":"2013"},{"key":"b8","series-title":"Emerg Infect Dis","first-page":"1119","article-title":"Invasive fresh water snail, China","volume":"13","author":"Wang","year":"2007"},{"key":"b9","series-title":"Am J Trop Med Hyg","first-page":"983","article-title":"Prevalence and intensity of infection with third stage larvae of Angiostrongylus cantonensis in mollusks from Northeast Thailand","volume":"80","author":"Tesana","year":"2009"},{"key":"b10","series-title":"Indian J Med Microbiol","first-page":"220","article-title":"Eosinophilic meningitis due to Angiostrongylus cantonensis","volume":"24","author":"Panackel","year":"2006"},{"key":"b11","series-title":"Am J Trop Med Hyg","first-page":"399","article-title":"Eosinophilic meningitis caused by Angiostrongylus cantonensis after ingestion of raw frogs","volume":"76","author":"Lai","year":"2007"},{"key":"b12","series-title":"N Engl J Med","first-page":"668","article-title":"An outbreak of eosinophilic meningitis caused by Angiostrongylus cantonensis in travelers returning from the Caribbean","volume":"346","author":"Slom","year":"2002"},{"key":"b13","series-title":"Am J Trop Med Hyg","first-page":"222","article-title":"Outbreak of eosinophilic meningitis associated with drinking raw vegetable juice in southern Taiwan","volume":"71","author":"Tsai","year":"2004"},{"key":"b14","series-title":"Studies on Angiostrongyliasis in Eastern Asia and Australia","first-page":"138","article-title":"Angiostrongyliasis: clinical features and human pathology","author":"Punyagupta","year":"1979"},{"key":"b15","series-title":"Food-Borne Parasitic Zoonoses: Fish and Plant Borne Parasites","first-page":"263","article-title":"Angiostrongyliasis","author":"Cross","year":"2007"},{"key":"b16","series-title":"Clin Microbiol Rev","first-page":"322","article-title":"Update on eosinophilic meningoencephalitis and its clinical relevance","volume":"22","author":"Graeff-Teixeira","year":"2009"},{"key":"b17","series-title":"Southeast Asian J Trop Med Public Health","first-page":"291","article-title":"The evaluation of the 29 and 31 kDa antigens in female Angiostrongylus cantonensis for serodiagnosis of human angiostrongyliasis","volume":"27","author":"Nuamtanong","year":"1996"},{"key":"b18","series-title":"Asian Pac J Allergy Immunol","first-page":"267","article-title":"Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis","volume":"19","author":"Maleewong","year":"2001"},{"key":"b19","series-title":"Parasitol Res","first-page":"425","article-title":"Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis","volume":"89","author":"Intapan","year":"2003"},{"key":"b20","series-title":"J Helminthol","first-page":"287","article-title":"A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human parastrongyliasis","volume":"78","author":"Eamsobhana","year":"2004"},{"key":"b21","series-title":"Trop Biomed","first-page":"121","article-title":"Evaluation of dot immunogold filtration assay (DIGFA) for rapid serodiagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea)","volume":"32","author":"Eamsobhana","year":"2015"},{"key":"b22","series-title":"Int J Infect Dis","first-page":"69","article-title":"Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis","volume":"73","author":"Eamsobhana","year":"2018"},{"key":"b23","series-title":"Hawaii J Med Public Health","first-page":"58","article-title":"Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report","volume":"72","author":"Morassutti","year":"2013"},{"key":"b24","series-title":"Trans R Soc Trop Med Hyg","first-page":"774","article-title":"The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection","volume":"80","author":"Elkins","year":"1986"},{"key":"b25","series-title":"Clin Infect Dis","first-page":"660","article-title":"Corticosteroid treatment of eosinophilic meningitis","volume":"31","author":"Chotmongkol","year":"2000"},{"key":"b26","series-title":"Parasitol Res","first-page":"201","article-title":"Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera","volume":"117","author":"Somboonpatarakun","year":"2018"},{"key":"b27","series-title":"FEBS Lett","first-page":"172","article-title":"A semi-empirical method for prediction of antigenic determinants on protein antigens","volume":"276","author":"Kolaskar","year":"1990"},{"key":"b28","series-title":"Pediatr Clin North Am","first-page":"861","article-title":"Predictive value and efficiency of laboratory testing","volume":"27","author":"Galen","year":"1980"},{"key":"b29","series-title":"Southeast Asian J Trop Med Public Health","first-page":"785","article-title":"Application of recombinant SMR-domain containing protein of Angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis","volume":"41","author":"Vitta","year":"2010"},{"key":"b30","series-title":"Parasitol Res","first-page":"2145","article-title":"Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis","volume":"110","author":"Li","year":"2012"},{"key":"b31","series-title":"Parasitol Res","first-page":"4115","article-title":"Cloning and expression of a 16-kDa recombinant protein from Angiostrongylus cantonensis for use in immunoblot diagnosis of human angiostrongyliasis","volume":"115","author":"Vitta","year":"2016"},{"key":"b32","series-title":"Mol Biochem Parasitol","first-page":"32","article-title":"Heterologous expression of three antigenic proteins from Angiostrongylus cantonensis: ES-7, Lec-5, and 14-3-3 in mammalian cells","volume":"221","author":"Cognato","year":"2018"},{"key":"b33","series-title":"Int Med Res J","first-page":"9","article-title":"Characterization of a 31-kDa specific antigen from Parastrongylus cantonensis (Nematoda: Metastrongylidae)","volume":"2","author":"Eamsobhana","year":"1998"},{"key":"b34","series-title":"Vector Borne Zoonotic Dis","first-page":"961","article-title":"The 31-kDa antigen of Angiostrongylus cantonensis comprises distinct antigenic glycoproteins","volume":"12","author":"Morassutti","year":"2012"},{"key":"b35","series-title":"Cell Mol Life Sci","first-page":"1679","article-title":"Galectins: matricellular glycan-binding proteins linking cell adhesion, migration, and survival","volume":"64","author":"Elola","year":"2007"},{"key":"b36","series-title":"Transpl Int","first-page":"118","article-title":"The role of indoleamine 2, 3-dioxygenase in transplantation","volume":"20","author":"Hainz","year":"2007"},{"key":"b37","series-title":"Mol Biochem Parasitol","first-page":"8","article-title":"The secretome of the filarial parasite, Brugia malayi: proteomic profile of adult excretory-secretory products","volume":"160","author":"Hewitson","year":"2008"},{"key":"b38","series-title":"Mol Biochem Parasitol","first-page":"305","article-title":"OvGa1BP, a filarial antigen with homology to vertebrate galactoside\u2013binding proteins","volume":"65","author":"Klion","year":"1994"},{"key":"b39","series-title":"Exp Parasitol","first-page":"26","article-title":"Characterization of Angiostrongylus cantonensis excretory-secretory proteins as potential diagnostic targets","volume":"130","author":"Morassutti","year":"2012"},{"key":"b40","series-title":"F1000 Biol Rep","first-page":"3","article-title":"Ocular immune privilege","volume":"2","author":"Zhou","year":"2010"}],"container-title":["The American Journal of Tropical Medicine and 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