{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:49Z","timestamp":1787031169882,"version":"build-2736575974"},"reference-count":31,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,4]]},"abstract":"<jats:p>\n                    We report a case of\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    infection in a young man who presented with cerebral infarction and shortly after admission developed bloody diarrhea with fever. A rapid diagnosis of severe\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    colitis was established through the use of a multiplex polymerase chain reaction enteropathogen stool panel. This result was unexpected in a patient native to the United States without known risk factors for amebiasis and negative stool microscopy examination for ova and parasites. Rapid diagnosis allowed prompt initiation of appropriate anti-amebic therapy and ultimately a good outcome in a condition that otherwise carries high morbidity and fatality.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.19-0237","type":"journal-article","created":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T03:01:41Z","timestamp":1572318101000},"page":"1380-1383","source":"Crossref","is-referenced-by-count":9,"title":["Case Report: Lower Gastrointestinal Bleeding due to Entamoeba histolytica Detected Early by Multiplex PCR: Case Report and Review of the Laboratory Diagnosis of Amebiasis"],"prefix":"10.4269","volume":"101","author":[{"given":"Gregory R.","family":"Madden","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie-Ann","family":"Shirley","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Pediatrics, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Townsend","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"Moonah","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Lancet","first-page":"209","article-title":"Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study","volume":"382","author":"Kotloff","year":"2013"},{"key":"b2","series-title":"PLoS One","first-page":"e0212791","article-title":"Approach to amoebic colitis: epidemiological, clinical and diagnostic considerations in a non-endemic context (Barcelona, 2007\u20132017)","volume":"14","author":"Roure","year":"2019"},{"key":"b3","series-title":"J Infect","first-page":"19","article-title":"A global study of pathogens and host risk factors associated with infectious gastrointestinal disease in returned international travellers","volume":"59","author":"Swaminathan","year":"2009"},{"key":"b4","series-title":"PLoS Negl Trop 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