{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:17:19Z","timestamp":1781705839993,"version":"3.54.5"},"reference-count":38,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,6]]},"abstract":"<jats:p>Chronic infections with the food-borne liver flukes, <jats:italic>Opisthorchis viverrini<\/jats:italic> or <jats:italic>Clonorchis sinensis<\/jats:italic>, associate with cholangiocarcinoma, bile duct cancer, which generally has a poor prognosis. We have produced a rapid and simple immunochromatographic test (ICT) kit for the diagnosis of opisthorchiasis and clonorchiasis by the detection of IgG antibodies in human infection sera. Sera from volunteers with proven opisthorchiasis and several other parasitic diseases and from healthy controls were evaluated for the presence of liver fluke infection\u2013specific antibodies using a preparation of excretory\u2013secretory antigen from adult stage <jats:italic>O. viverrini<\/jats:italic> absorbed onto ICT strips. Diagnostic values were compared with an ELISA. The diagnostic sensitivity, specificity, and positive and negative predictive values of the ELISA were 100%, 98.3%, 97.9%, and 100%, whereas those for the ICT were 94.6%, 91.2%, 89.7%, and 95.4%, respectively. There was 91.7% concordance between the ICT with ELISA, and differences in performance between the tests were not statistically significant (<jats:italic>P<\/jats:italic> &gt; 0.05). Twenty-seven of 30 (90%) of the clonorchiasis sera also were positive by ICT. This new ICT provides a facile, rapid test for point-of-care testing tool, which can be used at the bedside without the need for sophisticated equipment. Moreover, the ICT can be anticipated to supplement stool examination as a screening tool in the clinic for the diagnosis of opisthorchiasis and clonorchiasis, and in addition, it may be useful in screens of populations at risk of liver fluke infection\u2013associated cholangiocarcinoma.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0446","type":"journal-article","created":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T12:35:54Z","timestamp":1567600554000},"page":"1156-1160","source":"Crossref","is-referenced-by-count":21,"title":["Development of an Immunochromatographic Point-of-Care Test for Serodiagnosis of Opisthorchiasis and Clonorchiasis"],"prefix":"10.4269","volume":"101","author":[{"given":"Lakkhana","family":"Sadaow","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology, National Institute of Infectious Diseases, Ministry of Health, Labour and Welfare, Tokyo, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wannaporn","family":"Ittiprasert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, District of Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria H.","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, District of Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brindley","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, Research Center for Neglected Diseases of Poverty, School of Medicine and Health Science, George Washington University, Washington, District of Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Adv 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