{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T05:17:02Z","timestamp":1776921422769,"version":"3.51.2"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,2]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p>Cutaneous leishmaniasis (CL) is firmly established in South America. We aimed to assess the detection of IgG antibodies against 14 and\/or 16 kDa antigens by immunoblot (IB) for CL serological diagnosis in French Guiana, an area where many endemic pathogens could interfere with it. This study was performed retrospectively on sera from 141 patients at the Cayenne tertiary hospital: 30 were patients with confirmed CL, 71 were diagnosed with various other endemic pathogens, 11 were diagnosed with an autoimmune disease, and 29 controls had no history of CL. Antibodies bound to the 14 and\/or 16 kDa antigens in 27 of the 30 CL patients\u2019 sera and in 39 of the 111 non-CL patients\u2019 sera (26 from the infectious diseases group, four from the autoimmune diseases group, and nine from the dermatology department). The method tested showed a high sensitivity (90%) and a low specificity (66%), and a diagnosis odds ratio of 17.5 (95% CI [4.6\u201378.0]). This IB may be helpful to exclude the diagnosis of CL, prompting physicians to look for another diagnosis in the case of a negative IB.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0591","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T14:29:00Z","timestamp":1620052140000},"page":"2091-2096","source":"Crossref","is-referenced-by-count":2,"title":["Immunoblot for the Diagnosis of Cutaneous Leishmaniasis in French Guiana"],"prefix":"10.4269","volume":"104","author":[{"given":"Estelle","family":"Menu","sequence":"first","affiliation":[{"name":"1Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"},{"name":"2Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Institut Hospitalo-Universitaire, M\u00e9diterran\u00e9e Infection, Marseille, France;"},{"name":"3Aix Marseille Universit\u00e9, IRD, AP-HM, IHU-M\u00e9diterran\u00e9e Infection, UMR Vecteurs \u2013 Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Marseille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Blaizot","sequence":"additional","affiliation":[{"name":"4Department of Dermatology, Andr\u00e9e Rosemon Hospital, Cayenne, French Guiana;"},{"name":"5EA3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, University of French Guiana, Cayenne, French Guiana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Mary","sequence":"additional","affiliation":[{"name":"2Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Institut Hospitalo-Universitaire, M\u00e9diterran\u00e9e Infection, Marseille, France;"},{"name":"3Aix Marseille Universit\u00e9, IRD, AP-HM, IHU-M\u00e9diterran\u00e9e Infection, UMR Vecteurs \u2013 Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Marseille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Simon","sequence":"additional","affiliation":[{"name":"1Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"},{"name":"5EA3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, University of French Guiana, Cayenne, French Guiana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Adenis","sequence":"additional","affiliation":[{"name":"6Department of Internal Medicine, Andr\u00e9e Rosemon Hospital, Cayenne, French Guiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denis","family":"Blanchet","sequence":"additional","affiliation":[{"name":"1Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"},{"name":"5EA3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, University of French Guiana, Cayenne, French Guiana;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"L\u2019Ollivier","sequence":"additional","affiliation":[{"name":"2Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Institut Hospitalo-Universitaire, M\u00e9diterran\u00e9e Infection, Marseille, France;"},{"name":"3Aix Marseille Universit\u00e9, IRD, AP-HM, IHU-M\u00e9diterran\u00e9e Infection, UMR Vecteurs \u2013 Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Marseille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9phane","family":"Ranque","sequence":"additional","affiliation":[{"name":"2Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Institut Hospitalo-Universitaire, M\u00e9diterran\u00e9e Infection, Marseille, France;"},{"name":"3Aix Marseille Universit\u00e9, IRD, AP-HM, IHU-M\u00e9diterran\u00e9e Infection, UMR Vecteurs \u2013 Infections Tropicales et M\u00e9diterran\u00e9ennes (VITROME), Marseille, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magalie","family":"Demar","sequence":"additional","affiliation":[{"name":"1Laboratoire Hospitalo-Universitaire de Parasitologie-Mycologie, Centre Hospitalier Andr\u00e9e Rosemon, Cayenne, French Guiana;"},{"name":"5EA3593, Ecosyst\u00e8mes Amazoniens et Pathologies Tropicales, University of French Guiana, Cayenne, French Guiana;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2091.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/6\/article-p2091.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T00:40:46Z","timestamp":1628728846000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2091.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":0,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.19-0591","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}