{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:00:09Z","timestamp":1781524809632,"version":"3.54.1"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,6]]},"abstract":"<jats:p>Laboratory detection of malaria antigens has proved valuable for research and epidemiological purposes. We recently developed a bead-based multiplex antigen assay for pan-<jats:italic>Plasmodium<\/jats:italic> and <jats:italic>Plasmodium falciparum<\/jats:italic> targets. Here, we report integration of a <jats:italic>Plasmodium vivax<\/jats:italic>\u2013specific target to this multiplex panel: <jats:italic>P. vivax<\/jats:italic> lactate dehydrogenase (PvLDH). Within the multiplex panel, assay signal for purified PvLDH antigen titrated into the single-digit picogram range. Against a panel of polymerase chain reaction (PCR)-confirmed samples from acute <jats:italic>P. vivax<\/jats:italic> infections (<jats:italic>n<\/jats:italic> = 36), sensitivity was 91.7% in using PvLDH detection for identifying the presence of parasites. Specificity against a panel of persons with no <jats:italic>Plasmodium<\/jats:italic> infection (<jats:italic>n<\/jats:italic> = 44) was 100%, and specificity against a panel of PCR-confirmed <jats:italic>P. falciparum, Plasmodium malariae,<\/jats:italic> or <jats:italic>Plasmodium ovale<\/jats:italic> infections (<jats:italic>n<\/jats:italic> = 164) was 90.2%. Addition of this PvLDH capture and detection system into the multiplex antigen panel will now allow for sensitive screening for species identification of both <jats:italic>P. falciparum<\/jats:italic> and <jats:italic>P. vivax<\/jats:italic> in the laboratory.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0772","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T07:00:49Z","timestamp":1583218849000},"page":"1064-1067","source":"Crossref","is-referenced-by-count":7,"title":["Capture and Detection of Plasmodium vivax Lactate Dehydrogenase in a Bead-Based Multiplex Immunoassay"],"prefix":"10.4269","volume":"102","author":[{"given":"Eric","family":"Rogier","sequence":"first","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doug","family":"Nace","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Ljolje","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"},{"name":"CDC Fellowship, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi W.","family":"Lucchi","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatachalam","family":"Udhayakumar","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Aidoo","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"BMC Public Health","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1186\/s12889-017-4398-1","article-title":"Use of malaria RDTs in various health contexts across sub-Saharan Africa: a systematic review","volume":"17","author":"Boyce","year":"2017"},{"key":"b2","series-title":"J Clin Microbiol","first-page":"e00875-9","article-title":"Assessing performance of HRP2 antigen detection for malaria diagnosis in Mozambique","volume":"57","author":"Plucinski","year":"2019"},{"key":"b3","series-title":"Parasit Vectors","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1186\/s13071-019-3549-x","article-title":"Clearance dynamics of lactate dehydrogenase and aldolase following antimalarial treatment for Plasmodium falciparum infection","volume":"12","author":"Plucinski","year":"2019"},{"key":"b4","series-title":"J Infect Dis","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1093\/infdis\/jiy525","article-title":"Screening for Pfhrp2\/3-deleted Plasmodium falciparum, non-falciparum, and low-density malaria infections by a multiplex antigen assay","volume":"219","author":"Plucinski","year":"2019"},{"key":"b5","series-title":"J Clin Microbiol","doi-asserted-by":"publisher","first-page":"e00948-18","DOI":"10.1128\/JCM.00948-18","article-title":"Simultaneous quantification of Plasmodium antigens and host factor C-reactive protein in asymptomatic individuals with confirmed malaria by use of a novel multiplex immunoassay","volume":"57","author":"Jang","year":"2019"},{"key":"b6","series-title":"Malar J","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1186\/s12936-017-2101-8","article-title":"Malaria surveys using rapid diagnostic tests and validation of results using post hoc quantification of Plasmodium falciparum histidine-rich protein 2","volume":"16","author":"Plucinski","year":"2017"},{"key":"b7","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0172139","DOI":"10.1371\/journal.pone.0172139","article-title":"Bead-based immunoassay allows sub-picogram detection of histidine-rich protein 2 from Plasmodium falciparum and estimates reliability of malaria rapid diagnostic tests","volume":"12","author":"Rogier","year":"2017"},{"key":"b8","series-title":"J Vector Borne Dis","first-page":"281","article-title":"A prospective study from south India to compare the severity of malaria caused by Plasmodium vivax, P. falciparum and dual infection","volume":"52","author":"Mitra","year":"2015"},{"key":"b9","series-title":"Malar J","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1186\/s12936-019-2786-y","article-title":"Evidence of asymptomatic submicroscopic malaria in low transmission areas in Belaga district, Kapit division, Sarawak, Malaysia","volume":"18","author":"Jiram","year":"2019"},{"key":"b10","series-title":"BMC Public Health","first-page":"25","article-title":"Prevalence and associated risk factors of malaria among adults in East Shewa zone of Oromia regional state, Ethiopia: a cross-sectional study","volume":"18","author":"Tadesse","year":"2017"},{"key":"b11","series-title":"Malar J","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1186\/s12936-018-2545-5","article-title":"A new highly sensitive enzyme-linked immunosorbent assay for the detection of Plasmodium falciparum histidine-rich protein 2 in whole blood","volume":"17","author":"Jang","year":"2018"},{"key":"b12","series-title":"PLoS One","doi-asserted-by":"publisher","first-page":"e0159968","DOI":"10.1371\/journal.pone.0159968","article-title":"High Plasmodium malariae prevalence in an endemic area of the Colombian Amazon region","volume":"11","author":"Camargo-Ayala","year":"2016"},{"key":"b13","series-title":"Sci Rep","doi-asserted-by":"publisher","first-page":"9784","DOI":"10.1038\/s41598-019-46194-9","article-title":"Characterization of Plasmodium infections among inhabitants of rural areas in Gabon","volume":"9","author":"Woldearegai","year":"2019"},{"key":"b14","series-title":"Clin Microbiol Infect","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1016\/j.cmi.2018.09.009","article-title":"Genetic diversity and deletion of Plasmodium falciparum histidine-rich protein 2 and 3: a threat to diagnosis of P. falciparum malaria","volume":"25","author":"Gendrot","year":"2019"},{"key":"b15","series-title":"J Med Microbiol","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1099\/jmm.0.052506-0","article-title":"Malaria rapid diagnostic tests: challenges and prospects","volume":"62","author":"Mouatcho","year":"2013"},{"key":"b16","series-title":"Sci Transl Med","doi-asserted-by":"publisher","first-page":"eaan4479","DOI":"10.1126\/scitranslmed.aan4479","article-title":"A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir","volume":"11","author":"Tao","year":"2019"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/102\/5\/article-p1064.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/102\/5\/article-p1064.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/102\/5\/article-p1064.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T18:20:44Z","timestamp":1675707644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/102\/5\/article-p1064.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,6]]},"references-count":16,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.19-0772","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,6]]}}}