{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T01:06:50Z","timestamp":1774660010197,"version":"3.50.1"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,2]]},"DOI":"10.4269\/ajtmh.19-0798","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T07:01:00Z","timestamp":1595919660000},"page":"1032-1038","source":"Crossref","is-referenced-by-count":3,"title":["Transcutaneous Vaccination with Conjugate Typhoid Vaccine Vi-DT Induces Systemic, Mucosal, and Memory Anti-Polysaccharide Responses"],"prefix":"10.4269","volume":"103","author":[{"given":"Md Saruar","family":"Bhuiyan","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"},{"name":"International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anuj","family":"Kalsy","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Arifuzzaman","sequence":"additional","affiliation":[{"name":"International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richelle C.","family":"Charles","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts;"},{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason B.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Harvard Medical School, Boston, Massachusetts;"},{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B.","family":"Calderwood","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts;"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts;"},{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"name":"International Center for Diarrheal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward T.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts;"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts;"},{"name":"Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, Massachusetts;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0165-2427(02)00037-5","article-title":"Effect of transcutaneous immunization with co-administered antigen and cholera toxin on systemic and mucosal antibody responses in sheep","volume":"86","author":"Chen","year":"2002","journal-title":"Vet Immunol Immunopathol"},{"key":"b2","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1128\/IAI.01740-06","article-title":"Safety and immunogenicity of an enterotoxigenic Escherichia coli vaccine patch containing heat-labile toxin: use of skin pretreatment to disrupt the stratum corneum","volume":"75","author":"Glenn","year":"2007","journal-title":"Infect Immun"},{"key":"b3","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1016\/j.vaccine.2007.01.043","article-title":"Transcutaneous immunization with the heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC): protective efficacy in a double-blind, placebo-controlled challenge study","volume":"25","author":"McKenzie","year":"2007","journal-title":"Vaccine"},{"key":"b4","doi-asserted-by":"crossref","first-page":"4917","DOI":"10.1016\/j.vaccine.2009.06.040","article-title":"Transcutaneous immunization with a synthetic hexasaccharide-protein conjugate induces anti-Vibrio cholerae lipopolysaccharide responses in mice","volume":"27","author":"Rollenhagen","year":"2009","journal-title":"Vaccine"},{"key":"b5","doi-asserted-by":"crossref","first-page":"e0211784","DOI":"10.1371\/journal.pone.0211784","article-title":"Six-month follow up of a randomized clinical trial-phase I study in Indonesian adults and children: safety and immunogenicity of Salmonella typhi polysaccharide-diphtheria toxoid (Vi-DT) conjugate vaccine","volume":"14","author":"Medise","year":"2019","journal-title":"PLoS One"},{"key":"b6","doi-asserted-by":"crossref","first-page":"4","DOI":"10.4269\/ajtmh.18-0032","article-title":"Introductory article on global burden and epidemiology of typhoid fever","volume":"99","author":"Radhakrishnan","year":"2018","journal-title":"Am J Trop Med Hyg"},{"key":"b7","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.chom.2014.12.003","article-title":"Apical invasion of intestinal epithelial cells by Salmonella typhimurium requires villin to remodel the brush border actin cytoskeleton","volume":"17","author":"Lhocine","year":"2015","journal-title":"Cell Host Microbe"},{"key":"b8","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/S0140-6736(98)09001-1","article-title":"Typhoid fever in children aged less than 5 years","volume":"354","author":"Sinha","year":"1999","journal-title":"Lancet"},{"key":"b9","first-page":"CD001261","article-title":"Vaccines for preventing typhoid fever","volume":"5","author":"Milligan","year":"2018","journal-title":"Cochrane Database Syst Rev"},{"key":"b10","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1056\/NEJMoa0807521","article-title":"A cluster-randomized effectiveness trial of Vi typhoid vaccine in India","volume":"361","author":"Sur","year":"2009","journal-title":"N Engl J Med"},{"key":"b11","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/CVI.00266-09","article-title":"Physical and chemical characterization and immunologic properties of Salmonella enterica serovar typhi capsular polysaccharide-diphtheria toxoid conjugates","volume":"17","author":"Cui","year":"2010","journal-title":"Clin Vaccine Immunol"},{"key":"b12","doi-asserted-by":"crossref","first-page":"3794","DOI":"10.1016\/j.vaccine.2018.05.038","article-title":"Safety and immunogenicity of a Vi-DT typhoid conjugate vaccine: phase I trial in healthy Filipino adults and children","volume":"36","author":"Capeding","year":"2018","journal-title":"Vaccine"},{"key":"b13","doi-asserted-by":"crossref","first-page":"5834","DOI":"10.1128\/IAI.00438-06","article-title":"Transcutaneous immunization with toxin-coregulated pilin A induces protective immunity against Vibrio cholerae O1 El Tor challenge in mice","volume":"74","author":"Rollenhagen","year":"2006","journal-title":"Infect Immun"},{"key":"b14","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1128\/CVI.00311-09","article-title":"Salmonella enterica serovar Typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever","volume":"16","author":"Sheikh","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"b15","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1128\/CVI.05689-11","article-title":"Transcutaneous immunization with a Vibrio cholerae O1 Ogawa synthetic hexasaccharide conjugate following oral whole-cell cholera vaccination boosts vibriocidal responses and induces protective immunity in mice","volume":"19","author":"Tarique","year":"2012","journal-title":"Clin Vaccine Immunol"},{"key":"b16","doi-asserted-by":"crossref","first-page":"e0003881","DOI":"10.1371\/journal.pntd.0003881","article-title":"A cholera conjugate vaccine containing O-specific polysaccharide (OSP) of V. cholerae O1 inaba and recombinant fragment of tetanus toxin heavy chain (OSP:rTTHc) induces serum, memory and lamina proprial responses against OSP and is protective in mice","volume":"9","author":"Sayeed","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"key":"b17","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1084\/jem.160.2.521","article-title":"Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. Role in suppression of cytotoxic T lymphocyte response and viral persistence","volume":"160","author":"Ahmed","year":"1984","journal-title":"J Exp Med"},{"key":"b18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0022-1759(96)00146-9","article-title":"Limiting dilution analysis of virus-specific memory B cells by an ELISPOT assay","volume":"199","author":"Slifka","year":"1996","journal-title":"J Immunol Methods"},{"key":"b19","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1086\/423327","article-title":"Successful induction of protective antibody responses against Haemophilus influenzae type b and diphtheria after transcutaneous immunization with the glycoconjugate polyribosyl ribitol phosphate-cross-reacting material 197 vaccine","volume":"190","author":"Mawas","year":"2004","journal-title":"J Infect Dis"},{"key":"b20","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1172\/JCI20261","article-title":"Transcutaneous immunization induces mucosal CTLs and protective immunity by migration of primed skin dendritic cells","volume":"113","author":"Belyakov","year":"2004","journal-title":"J Clin Invest"},{"key":"b21","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1016\/S0264-410X(00)00059-1","article-title":"The induction of systemic and mucosal immune responses to antigen-adjuvant compositions administered into the skin: alterations in the migratory properties of dendritic cells appears to be important for stimulating mucosal immunity","volume":"18","author":"Enioutina","year":"2000","journal-title":"Vaccine"},{"key":"b22","doi-asserted-by":"crossref","first-page":"178.10","DOI":"10.4049\/jimmunol.190.Supp.178.10","article-title":"cAMP-based adjuvants (LT, mLT, dmLT) promote Th17 and germinal center responses through dendritic cells, while direct CD4 T-cell contact induces a T-suppressor phenotype (P4512)","volume":"190","author":"Norton","year":"2013","journal-title":"J Immunol"},{"key":"b23","doi-asserted-by":"crossref","first-page":"e00215-18","DOI":"10.1128\/mSphere.00215-18","article-title":"The mucosal vaccine adjuvant LT(R192G\/L211A) or dmLT","volume":"3","author":"Clements","year":"2018","journal-title":"mSphere"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T23:06:49Z","timestamp":1696460809000},"score":1,"resource":{"primary":{"URL":"https:\/\/ajtmh.org\/doi\/10.4269\/ajtmh.19-0798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,2]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9,2]]}},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.19-0798","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,2]]}}}