{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T14:29:36Z","timestamp":1748528976696},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,6]]},"abstract":"<jats:p>Invasion of human erythrocytes by merozoites of <jats:italic>Plasmodium knowlesi<\/jats:italic> involves interaction between the <jats:italic>P. knowlesi<\/jats:italic> Duffy binding protein alpha region II (PkDBP\u03b1II) and Duffy antigen receptor for chemokines (DARCs) on the erythrocytes. Information is scarce on the binding level of PkDBP\u03b1II to different Duffy antigens, Fy<jats:sup>a<\/jats:sup> and Fy<jats:sup>b<\/jats:sup>. This study aims to measure the binding level of two genetically distinct PkDBP\u03b1II haplotypes to Fy(a+b\u2212) and Fy(a+b+) human erythrocytes using erythrocyte-binding assay. The binding level of PkDBP\u03b1II of Peninsular Malaysian and Malaysian Borneon haplotypes to erythrocytes was determined by counting the number of rosettes formed in the assay. Overall, the Peninsular Malaysian haplotype displayed higher binding activity than the Malaysian Borneon haplotype. Both haplotypes exhibit the same preference to Fy(a+b+) compared with Fy(a+b\u2212), hence justifying the vital role of Fy<jats:sup>b<\/jats:sup> in the binding to PkDBP\u03b1II. Further studies are needed to investigate the <jats:italic>P. knowlesi<\/jats:italic> susceptibility on individuals with different Duffy blood groups.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0836","type":"journal-article","created":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T07:00:54Z","timestamp":1584428454000},"page":"1068-1071","source":"Crossref","is-referenced-by-count":1,"title":["Two Genetically Distinct Plasmodium knowlesi Duffy Binding Protein Alpha Region II (PkDBP\u03b1II) Haplotypes Demonstrate Higher Binding Level to Fy(a+b+) Erythrocytes than Fy(a+b--) Erythrocytes"],"prefix":"10.4269","volume":"102","author":[{"given":"Chin Chin","family":"Liew","sequence":"first","affiliation":[{"name":"Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mun Yik","family":"Fong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei Wen","family":"Cheong","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Malar J","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1186\/1475-2875-13-168","article-title":"High proportion of knowlesi malaria in recent malaria cases in Malaysia","volume":"13","author":"Yusof","year":"2014"},{"key":"b2","series-title":"J Exp 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