{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:44:46Z","timestamp":1790239486584,"version":"4.1.0"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,6]]},"abstract":"<jats:p>Artemether\u2013lumefantrine (AL) is the first-line treatment for uncomplicated <jats:italic>Plasmodium falciparum<\/jats:italic> infection in Colombia. To assess AL efficacy for uncomplicated falciparum malaria in Quibdo, Choco, Colombia, we conducted a 28-day therapeutic efficacy study (TES) following the WHO guidelines. From July 2018 to February 2019, febrile patients aged 5\u201365 years with microscopy-confirmed <jats:italic>P. falciparum<\/jats:italic> mono-infection and asexual parasite density of 250\u2013100,000 parasites\/\u00b5L were enrolled and treated with a supervised 3-day course of AL. The primary endpoint was adequate clinical and parasitological response (ACPR) on day 28. We attempted to use polymerase chain reaction (PCR) genotyping to differentiate reinfection and recrudescence, and conducted genetic testing for antimalarial resistance\u2013associated genes. Eighty-eight patients consented and were enrolled; four were lost to follow-up or missed treatment doses. Therefore, 84 (95.5%) participants reached a valid endpoint: treatment failure or ACPR. No patient remained microscopy positive for malaria on day 3, evidence of delayed parasite clearance and artemisinin resistance. One patient had recurrent infection (12 parasites\/\u00b5L) on day 28. Uncorrected ACPR rate was 98.8% (83\/84) (95% CI: 93.5\u2013100%). The recurrent infection sample did not amplify during molecular testing, giving a PCR-corrected ACPR of 100% (83\/83) (95% CI: 95.7\u2013100%). No <jats:italic>P. falciparum kelch<\/jats:italic> 13 polymorphisms associated with artemisinin resistance were identified. Our results support high AL efficacy for falciparum malaria in Choco. Because of the time required to conduct TESs in low-endemic settings, it is important to consider complementary alternatives to monitor antimalarial efficacy and resistance.<\/jats:p>","DOI":"10.4269\/ajtmh.19-0954","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T07:00:58Z","timestamp":1582614058000},"page":"1056-1063","source":"Crossref","is-referenced-by-count":21,"title":["Artemether\u2013Lumefantrine Efficacy for the Treatment of Uncomplicated Plasmodium falciparum Malaria in Choco, Colombia after 8 Years as First-Line Treatment"],"prefix":"10.4269","volume":"102","author":[{"given":"Mario J.","family":"Olivera","sequence":"first","affiliation":[{"name":"Grupo de Parasitolog\u00eda, Instituto Nacional de Salud, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Patricia","family":"Guerra","sequence":"additional","affiliation":[{"name":"Grupo de Parasitolog\u00eda, Instituto Nacional de Salud, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliana Jazmin","family":"Cortes","sequence":"additional","affiliation":[{"name":"Grupo de Parasitolog\u00eda, Instituto Nacional de Salud, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Z.","family":"Horth","sequence":"additional","affiliation":[{"name":"Epidemic Intelligence Service, Center for Surveillance, Epidemiology, and Laboratory Services, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Padilla","sequence":"additional","affiliation":[{"name":"Ministerio de Salud y Protecci\u00f3n Social, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Novoa","sequence":"additional","affiliation":[{"name":"Pan-American Health Organization, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda de la Paz","family":"Ade","sequence":"additional","affiliation":[{"name":"Department of Communicable Diseases and Environmental Determinants of Health, Pan-American Health Organization, Washington, District of Columbia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Ljolje","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi W.","family":"Lucchi","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilmer","family":"Marqui\u00f1o","sequence":"additional","affiliation":[{"name":"Pan-American Health Organization, Bogota, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Renteria","sequence":"additional","affiliation":[{"name":"Laboratorio Departamental de Salud P\u00fablica de Choco, Quibdo, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilman","family":"Yurgaky","sequence":"additional","affiliation":[{"name":"Hospital Ismael Roldan Valencia ESE, Quibdo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Macedo de Oliveira","sequence":"additional","affiliation":[{"name":"Malaria Branch, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"World Malaria Report 2018","author":"World Health Organization","year":"2018"},{"key":"b2","series-title":"Evento Malaria","author":"Instituto Nacional de Salud","year":"2018"},{"key":"b3","series-title":"Guidelines for the Treatment of Malaria","author":"World Health Organization","year":"2015"},{"key":"b4","series-title":"Antimalarial Drug Combination Therapy. 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