{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T11:57:56Z","timestamp":1761998276720,"version":"build-2065373602"},"reference-count":42,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,9,1]]},"abstract":"<jats:p>A genomic library of a savanna isolate of <jats:italic>Onchocerca volvulus<\/jats:italic> was screened to detect recombinant plasm ids containing highly repeated DNA sequences of this parasite. Four recombinant plasmids were identified which hybridized specifically to <jats:italic>Onchocerca<\/jats:italic> DNA, but not to DNA from humans, black flies, <jats:italic>Brugia malayi<\/jats:italic>, <jats:italic>B. pahangi<\/jats:italic>, or <jats:italic>Wuchereria bancrofti<\/jats:italic>. The recombinant plasm ids had a low level of homology to <jats:italic>Dirofilaria immitis<\/jats:italic>. All recombinant plasmids contain related DNA sequences based on Southern hybridization analysis. Sequences related to these recombinant plasmids are present in different geographic isolates of <jats:italic>O. volvulus<\/jats:italic> and <jats:italic>O. ochengi<\/jats:italic>, an animal parasite. Two of the recombinant plasmids contain sequences also found in <jats:italic>O. lienalis<\/jats:italic>. One recombinant plasmid, puOvs3, has been characterized in detail, including DNA sequence determination. Radiolabeled puOvs3 is able to detect 100 pg of genomic DNA isolated from <jats:italic>O. volvulus<\/jats:italic> worms from both savanna and forest regions. It can differentiate <jats:italic>O. volvulus<\/jats:italic> from <jats:italic>O. ochengi<\/jats:italic> by Southern blot analysis.<\/jats:p>","DOI":"10.4269\/ajtmh.1987.37.376","type":"journal-article","created":{"date-parts":[[2017,5,10]],"date-time":"2017-05-10T17:05:05Z","timestamp":1494435905000},"page":"376-384","source":"Crossref","is-referenced-by-count":32,"title":["Characterization of an Onchocerca-Specific DNA Clone from Onchocerca Volvulus"],"prefix":"10.4269","volume":"37","author":[{"given":"Jyotsna S.","family":"Shah","sequence":"first","affiliation":[{"name":"Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Karam","sequence":"additional","affiliation":[{"name":"* Onchocerciasis Control Programme, B.P. 549, Ouagadougou, Burkina Faso"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy F.","family":"Piessens","sequence":"additional","affiliation":[{"name":"Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dyann F.","family":"Wirth","sequence":"additional","affiliation":[{"name":"Department of Tropical Public Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Br. 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