{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:40:57Z","timestamp":1763037657287},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,3]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Scrub typhus is an acute infectious disease caused by the bacterium <jats:italic>Orientia tsutsugamushi<\/jats:italic>, which is widely distributed in northern, southern, and eastern Asia. Early diagnosis is essential because the average case fatality rate is usually &gt;10% but can be as high as 45% if antimicrobial treatment is delayed. Although an <jats:italic>O. tsutsugamushi<\/jats:italic> 56-kDa type-specific antigen (TSA) is commonly used for serological diagnosis of scrub typhus, the 56-kDa TSA shows variations among <jats:italic>O. tsutsugamushi<\/jats:italic> strains, which may lead to poor diagnostic results. Therefore, the discovery of new antigenic proteins may improve diagnostic accuracy. In this study, we identified an <jats:italic>O. tsutsugamushi<\/jats:italic> 27 kDa antigen through an immunoinformatic approach and verified its diagnostic potential using patient samples. Compared with the <jats:italic>O. tsutsugamushi<\/jats:italic> 56-kDa antigen, the new 27-kDa antigen showed better diagnostic specificity with similar diagnostic sensitivity. Therefore, the <jats:italic>O. tsutsugamushi<\/jats:italic> 27-kDa antigen shows potential as a novel serological diagnostic antigen for scrub typhus, providing higher diagnostic accuracy for <jats:italic>O. tsutsugamushi<\/jats:italic> than the 56-kDa antigen.<\/jats:p>","DOI":"10.4269\/ajtmh.20-0129","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T14:21:16Z","timestamp":1632147676000},"page":"1356-1361","source":"Crossref","is-referenced-by-count":5,"title":["Identification of a Novel Antigen for Serological Diagnosis of Scrub Typhus"],"prefix":"10.4269","volume":"105","author":[{"given":"Wooyoung","family":"Kim","sequence":"first","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Yeop","family":"Lee","sequence":"additional","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung Il","family":"Kim","sequence":"additional","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"},{"name":"3Department of Bio-Analysis Science, University of Science & Technology, Daejeon, Republic of Korea,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In-Kook","family":"Sohng","sequence":"additional","affiliation":[{"name":"4Manufacture Business Division Curebio Ltd, Seoul, Republic of Korea,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun Cheol","family":"Park","sequence":"additional","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangmi","family":"Jun","sequence":"additional","affiliation":[{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"},{"name":"5Center for Research Equipment, Korea Basic Science Institute, Cheongju, Republic of Korea;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Seop","family":"Lee","sequence":"additional","affiliation":[{"name":"6Department of Internal Medicine, Jeonbuk National University Medical School, Jeonju, Republic of Korea;"},{"name":"7Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmond Changkyun","family":"Park","sequence":"additional","affiliation":[{"name":"1Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju, Republic of Korea;"},{"name":"2Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea;"},{"name":"3Department of Bio-Analysis Science, University of Science & Technology, Daejeon, Republic of Korea,"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1356.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/105\/5\/article-p1356.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T14:33:29Z","timestamp":1635950009000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1356.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-0129","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]}}}