{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T08:30:48Z","timestamp":1742805048697},"reference-count":12,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Leptospirosis is an important zoonotic disease with worldwide distribution and nonspecific clinical manifestation. We report a case of fatal leptospirosis in a previously healthy woman with a causative agent. A young adult Indian woman was brought in dead to the forensic department. Ten days before, she developed fever, dizziness with headache, myalgia, diarrhea, and vomiting. Routine inquest and autopsy were performed on the deceased, revealing hemorrhagic lungs with extensive intra-alveolar hemorrhages, pale liver with dissociation and separation of hepatocyte plates, and edematous brain with histiocyte and lymphocyte infiltration in the parenchyma and meninges. Heart tissue depicts myocarditis and pericarditis inflammatory changes. Cerebrospinal fluid (CSF) was turbid in appearance with mildly elevated leukocytes, predominantly lymphocytes. Real-time PCR targeting lipL32 gene of pathogenic <jats:italic>Leptospira<\/jats:italic> was detected in the blood, CSF, brain, kidney, heart, and liver. The genetic profile of the causative agent was ST149 (multi-locus sequence typing Scheme 3). This study illustrates the usefulness of <jats:italic>Leptospira<\/jats:italic> PCR assay in postmortem diagnosis and addresses the need for further surveillance to identify the epidemiological link of the disease.<\/jats:p>","DOI":"10.4269\/ajtmh.20-0267","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T07:07:47Z","timestamp":1607411267000},"page":"216-218","source":"Crossref","is-referenced-by-count":5,"title":["Case Report: Fatal Human Leptospirosis Caused by Leptospira interrogans Genotype ST149"],"prefix":"10.4269","volume":"104","author":[{"given":"Mohan","family":"Rao","sequence":"first","affiliation":[{"name":"1Leptospirosis Reference Laboratory, Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fairuz","family":"Amran","sequence":"additional","affiliation":[{"name":"1Leptospirosis Reference Laboratory, Bacteriology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameera Ashyila","family":"Kamaruzaman","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Selayang Hospital, Lebuhraya Selayang, Kepong, Batu Caves, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Habib Abdul","family":"Hakim Esa","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Selayang Hospital, Lebuhraya Selayang, Kepong, Batu Caves, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahneez","family":"Abdul Hameed","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Selayang Hospital, Lebuhraya Selayang, Kepong, Batu Caves, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noor Afidah","family":"Mohamed Shabery","sequence":"additional","affiliation":[{"name":"2Department of Pathology, Selayang Hospital, Lebuhraya Selayang, Kepong, Batu Caves, Selangor, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","first-page":"296","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Curr Opin Infect Dis"},{"key":"b2","first-page":"1","volume-title":"Report of the First Meeting of the Leptospirosis Burden Epidemiology Reference Group","year":"2010"},{"key":"b3","doi-asserted-by":"crossref","first-page":"58","DOI":"10.3389\/fimmu.2017.00058","article-title":"Animal models of leptospirosis: of mice and hamsters","volume":"8","author":"Gomes-Solecki","year":"2017","journal-title":"Front Immunol"},{"key":"b4","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/1476-0711-5-28","article-title":"Multilocus sequence typing method for identification and genotypic classification of pathogenic 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Spain","volume":"5","author":"L\u00f3pez","year":"2019","journal-title":"Heliyon"},{"key":"b9","doi-asserted-by":"crossref","first-page":"e00956-16","DOI":"10.1128\/genomeA.00956-16","article-title":"Draft genome sequence of Leptospira interrogans serovar Bataviae strain LepIMR 22 isolated from a Rodent in Johor, Malaysia","volume":"4","author":"Amran","year":"2016","journal-title":"Genome Announc"},{"key":"b10","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1007\/s10393-017-1234-0","article-title":"Retrospective study of leptospirosis in Malaysia","volume":"14","author":"Garba","year":"2017","journal-title":"Ecohealth"},{"key":"b11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13071-016-1400-1","article-title":"Determination of Leptospira borgpetersenii serovar Javanica and Leptospira interrogans serovar Bataviae as the persistent Leptospira serovars circulating in the urban rat populations in Peninsular Malaysia","volume":"9","author":"Benacer","year":"2016","journal-title":"Parasit Vectors"},{"key":"b12","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1093\/cid\/cis035","article-title":"Utility of quantitative polymerase chain reaction in leptospirosis diagnosis: association of level of leptospiremia and clinical manifestations in Sri Lanka","volume":"54","author":"Agampodi","year":"2012","journal-title":"Clin Infect Dis"}],"container-title":["The American Journal of Tropical Medicine and 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