{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:22:56Z","timestamp":1790709776665,"version":"4.1.0"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,2]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p>Despite the dwindling number of visceral leishmaniasis (VL) cases in India, there is an urgent need for early and unequivocal diagnostics for controlling and preventing the reemergence of VL. Post\u2013kala-azar dermal leishmaniasis (PKDL), a dermal sequela of VL, serves as a reservoir of the parasite. Diagnosis of PKDL, especially the macular variant, is challenging and poses impediment toward attainment of VL elimination. In this study, a real-time fluorimetry loop-mediated isothermal amplification (RealAmp) assay has been established for the detection of different clinical manifestations of leishmaniasis. The study included 150 leishmaniasis patients (25 VL, 25 cutaneous leishmaniasis [CL], and 100-PKDL) along with 120 controls. The assay demonstrated sensitivity of 100% (95% CI: 86.68\u2013100) for diagnosis of VL and PKDL (95% CI: 79.61\u2013100) and 96% (95% CI: 86.68\u2013100) for CL with 100% specificity. Moreover, considering the cardinal role of PKDL, diagnosis using minimally invasive slit aspirate was explored, which demonstrated remarkable sensitivity of 96% (95% CI: 87.64\u201398.47). As a test of cure for PKDL, RealAmp successfully detected parasite in two of posttreatment cases who later reported relapse on follow-up. Also, direct sample lysis using slit aspirate was attempted in a small group that yielded sensitivity of 89% (95% CI: 67.20\u201396.90). RealAmp depicted excellent diagnostic accuracy in the diagnosis of leishmaniasis in concordance with the established SYBR Green I\u2013based (Molecular Probes, Eugene, OR) visual loop-mediated isothermal amplification (LAMP) and the reference comparator real-time PCR. The study endorsed the employment of LAMP either as visual-LAMP or RealAmp for an accurate and expeditious diagnosis of PKDL and as a tool for assessment of cure.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1057","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T13:06:27Z","timestamp":1618837587000},"page":"2097-2107","source":"Crossref","is-referenced-by-count":12,"title":["Real-Time Fluorimetry Loop-Mediated Isothermal Amplification for Diagnosis of Leishmaniasis and as a Tool for Assessment of Cure for Post\u2013Kala-Azar Dermal Leishmaniasis"],"prefix":"10.4269","volume":"104","author":[{"given":"Keerti Kaumudee","family":"Dixit","sequence":"first","affiliation":[{"name":"1ICMR-National Institute of Pathology (NIOP), Safdarjung Hospital Campus, New Delhi, India;"},{"name":"2Faculty of Health and Biological Sciences, Symbiosis International (Deemed University), Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesh","family":"Ramesh","sequence":"additional","affiliation":[{"name":"3Department of Dermatology and STD, Safdarjung Hospital, Vardhman Mahavir Medical College, New Delhi, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratan","family":"Gupta","sequence":"additional","affiliation":[{"name":"4Department of Paediatrics, Safdarjung Hospital, Vardhman Mahavir Medical College, New Delhi, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narendra Singh","family":"Negi","sequence":"additional","affiliation":[{"name":"5Department of Medicine, Safdarjung Hospital, Vardhman Mahavir Medical College, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruchi","family":"Singh","sequence":"additional","affiliation":[{"name":"1ICMR-National Institute of Pathology (NIOP), Safdarjung Hospital Campus, New Delhi, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Salotra","sequence":"additional","affiliation":[{"name":"1ICMR-National Institute of Pathology (NIOP), Safdarjung Hospital Campus, New Delhi, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2097.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/6\/article-p2097.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T16:39:41Z","timestamp":1622651981000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2097.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":0,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1057","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}