{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T09:17:05Z","timestamp":1793611025183,"version":"4.5.0"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,3]]},"abstract":"ABSTRACT.<\/jats:title>\n \n Evidence on the effectiveness of low-cost, sustainable biological vector control tools for\n Aedes<\/jats:italic>\n mosquitoes is limited. Therefore, the purpose of this trial was to estimate the impact of guppy fish in combination with the larvicide pyriproxyfen (PPF) (Sumilarv\n \u00ae<\/jats:sup>\n 2MR) and communication for behavioral impact (COMBI) activities to reduce entomological indices in Cambodia. In this cluster randomized, controlled superiority trial, 30 clusters comprised of one or more villages each was allocated in a 1:1:1 ratio to receive either 1) all three interventions (guppies, PPF, and COMBI), 2) two interventions (guppies and COMBI), or 3) control (standard vector control). Entomological surveys among 40 randomly selected households per cluster were carried out quarterly. The primary outcome was the population abundance of adult female\n Aedes<\/jats:italic>\n mosquitoes trapped using adult resting collections. In the primary analysis, adult female\n Aedes<\/jats:italic>\n abundance and mosquito infection rates was aggregated over follow-up time points to give a single rate per cluster. These data were analyzed by negative binomial regression, yielding abundance ratios (ARs). The number of\n Aedes<\/jats:italic>\n females was reduced roughly by half compared with the control in both the guppy, PPF, and COMBI arm (AR = 0.54; 95% CI, 0.34\u20130.85;\n P<\/jats:italic>\n = 0.0073); and the guppy and COMBI arm (AR = 0.49; 95% CI, 0.31\u20130.77;\n P<\/jats:italic>\n = 0.0021). The effectiveness demonstrated and extremely low cost of including fish rearing in community-based health structures suggest they should be considered as a vector control tool as long as the benefits outweigh any potential environmental concerns. Sumilarv\n \u00ae<\/jats:sup>\n 2MR was also highly accepted and preferred over current vector control tools used in Cambodia.\n <\/jats:p>","DOI":"10.4269\/ajtmh.20-1088","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T09:20:46Z","timestamp":1631006446000},"page":"1265-1276","source":"Crossref","is-referenced-by-count":18,"title":["Field Efficacy of Larvivorous Fish and Pyriproxyfen Combined with Community Engagement on Dengue Vectors in Cambodia: A Randomized Controlled Trial"],"prefix":"10.4269","volume":"105","author":[{"given":"John Christian","family":"Hustedt","sequence":"first","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"},{"name":"2MRC Tropical Epidemiology Group, Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dyna","family":"Doum","sequence":"additional","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanney","family":"Keo","sequence":"additional","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sokha","family":"Ly","sequence":"additional","affiliation":[{"name":"3Department of Vector Control, Cambodian National Dengue Control Program, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BunLeng","family":"Sam","sequence":"additional","affiliation":[{"name":"3Department of Vector Control, Cambodian National Dengue Control Program, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vibol","family":"Chan","sequence":"additional","affiliation":[{"name":"4Department of Climate Change and Health, World Health Organization, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal","family":"Alexander","sequence":"additional","affiliation":[{"name":"2MRC Tropical Epidemiology Group, Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Bradley","sequence":"additional","affiliation":[{"name":"2MRC Tropical Epidemiology Group, Department of Infectious Disease Epidemiology, London School of Hygiene and Tropical Medicine, London, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Liverani","sequence":"additional","affiliation":[{"name":"5Department of Global Health and Development, London School of Hygiene and Tropical Medicine, London, United Kingdom;"},{"name":"6School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didot Budi","family":"Prasetyo","sequence":"additional","affiliation":[{"name":"7Department of Entomology, US Naval Medical Research Unit-2, Phnom Penh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agus","family":"Rachmat","sequence":"additional","affiliation":[{"name":"7Department of Entomology, US Naval Medical Research Unit-2, Phnom Penh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shafique","sequence":"additional","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Lopes","sequence":"additional","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leang","family":"Rithea","sequence":"additional","affiliation":[{"name":"3Department of Vector Control, Cambodian National Dengue Control Program, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Hii","sequence":"additional","affiliation":[{"name":"1Epidemiology Department, Malaria Consortium, Phnom Penh, Cambodia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1265.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/105\/5\/article-p1265.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T10:33:14Z","timestamp":1635935594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/105\/5\/article-p1265.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1088","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.15.097782","asserted-by":"object"}]},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]}}}