{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:38:30Z","timestamp":1788514710623,"version":"build-2803163510"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,4]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Human babesiosis is a rare disease, caused by <jats:italic>Babesia<\/jats:italic> species and commonly transmitted by tick bite. Although human babesiosis is known to be asymptomatic in immunocompetent hosts, clinical cases of severe babesiosis have been reported from splenectomized or immunocompromised individuals. To our knowledge, only one case of human babesiosis in India has been previously reported. Here, we report a case of severe babesiosis with high parasitemia (\u223c70%) in a 30-year-old asplenic farmer. The patient presented with fever, yellowish discoloration of skin, oliguria, and anemia; he eventually developed multiorgan failure syndrome and died. Peripheral blood films were prepared and used to confirm the presence of piroplasms by microscopy. Total DNA isolated from blood was used for 18S ribosomal RNA gene fragment amplification by polymerase chain reaction, which was subjected to Sanger sequencing. Although 18S sequence indicated that the <jats:italic>Babesia<\/jats:italic> species infecting the patient was similar to that of other <jats:italic>Babesia<\/jats:italic> species originating from wild mammals, species identification could not be done. Phylogenetic analysis revealed that the patient-derived pathogen is distinct because it forms a separate clade in the cladogram.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1118","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T14:01:37Z","timestamp":1645452097000},"page":"1421-1425","source":"Crossref","is-referenced-by-count":7,"title":["Case Report: A Fatal Case of Babesiosis in a Splenectomized Male Patient from Western India"],"prefix":"10.4269","volume":"106","author":[{"given":"Ravi","family":"Godbole","sequence":"first","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avantika","family":"Gaur","sequence":"additional","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Nayar","sequence":"additional","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kala Gnanasekaran","family":"Kiruthiga","sequence":"additional","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep","family":"D\u2019Costa","sequence":"additional","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumma","family":"Manchanda","sequence":"additional","affiliation":[{"name":"1Department of Pathology, King Edward Memorial Hospital, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajinkya","family":"Khilari","sequence":"additional","affiliation":[{"name":"2Biochemical Sciences Division, National Chemical Laboratory, Pune, India;"},{"name":"3Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanasekaran","family":"Shanmugam","sequence":"additional","affiliation":[{"name":"2Biochemical Sciences Division, National Chemical Laboratory, Pune, India;"},{"name":"3Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalpana D.","family":"Muglikar","sequence":"additional","affiliation":[{"name":"4Western Regional Disease Diagnostic Laboratory, Pune, India;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnendu","family":"Kundu","sequence":"additional","affiliation":[{"name":"5Department of Veterinary Parasitology, Faculty of Veterinary and Animal Science, Rajiv Gandhi South Campus, Banaras Hindu University, Barkachha, Mirzapur, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1421.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/106\/5\/article-p1421.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T11:40:06Z","timestamp":1651664406000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/5\/article-p1421.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,4]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1118","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,4]]}}}