{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:09:52Z","timestamp":1777579792554,"version":"3.51.4"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Melioidosis, caused by <jats:italic>Burkholderia pseudomallei<\/jats:italic>, is increasingly recognized in several regions of the globe. The present study was performed to identify and determine the frequency of <jats:italic>B. pseudomallei<\/jats:italic> infection in localized pyogenic lesions in eastern India and describe their clinico-microbiological profile. Pus samples were subjected to standard microbiological techniques for isolation and identification of various bacteria, including <jats:italic>B. pseudomallei<\/jats:italic>, which were confirmed by PCR. The clinical and demographic details of patients with melioidosis and antimicrobial susceptibility pattern of <jats:italic>B. pseudomallei<\/jats:italic> isolates were analyzed. Of 245 samples, 126 (51.4%) were culture positive, yielding 137 isolates. <jats:italic>Staphylococcus aureus<\/jats:italic> was the predominant pathogen accounting for 54 (39.4%) isolates, followed by <jats:italic>B. pseudomallei<\/jats:italic> accounting for 34 (24.8%) isolates. The mean age of the patients with melioidosis was 39.1 years, with males (24\/34; 70.6%) being affected more than females (10\/34; 29.4%). A majority of the patients were laborers (12\/34; 35.3), followed by homemakers (8\/34; 23.5%). Head and neck abscesses (35.3%) were the most common presentation followed by pyogenic lesions of the musculoskeletal system (32.3%) and deep organ abscesses (23.5%). Clinical resolution of infection was observed in 31 (91.2%) patients, relapse in two (5.9%) patients, and death in one (2.9%) patient, respectively. Susceptibility testing revealed all <jats:italic>B. pseudomallei<\/jats:italic> isolates to be completely susceptible to the following antimicrobials: ceftazidime, trimethoprim\u2013sulfamethoxazole, imipenem, and doxycycline, with one (2.9%) resistant to amoxicillin\u2013clavulanic acid. <jats:italic>Burkholderia pseudomallei<\/jats:italic> is an emerging etiological agent of localized pyogenic infections in eastern India, affecting a mainly adult male population. An increased vigilance along with appropriate diagnostic techniques helps in accurate diagnosis facilitating appropriate therapy.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1386","type":"journal-article","created":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T18:37:19Z","timestamp":1612204639000},"page":"1252-1259","source":"Crossref","is-referenced-by-count":11,"title":["Identification and Characterization of Burkholderia pseudomallei from Localized Pyogenic Infections in Eastern India: A Clinico-Microbiological Study"],"prefix":"10.4269","volume":"104","author":[{"given":"Prashanth","family":"Purushotham","sequence":"first","affiliation":[{"name":"1Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srujana","family":"Mohanty","sequence":"additional","affiliation":[{"name":"1Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preetam","family":"Chappity","sequence":"additional","affiliation":[{"name":"2Department of ENT, All India Institute of Medical Sciences, Bhubaneswar, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tushar Subhadarshan","family":"Mishra","sequence":"additional","affiliation":[{"name":"3Department of General Surgery, All India Institute of Medical Sciences, Bhubaneswar, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashoka","family":"Mahapatra","sequence":"additional","affiliation":[{"name":"1Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/4\/article-p1252.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/4\/article-p1252.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T12:34:24Z","timestamp":1617798864000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/4\/article-p1252.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,7]]},"references-count":0,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1386","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,7]]}}}