{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T11:54:07Z","timestamp":1679399647908},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Antibody cross-reactivity confounds testing for dengue virus (DENV) and Zika virus (ZIKV). We evaluated anti-DENV and anti-ZIKV IgG detection using a multiplex serological platform (the pGOLD assay, Nirmidas, Palo Alto, CA) in patients from the Asunci\u00f3n metropolitan area in Paraguay, which experiences annual DENV outbreaks but has reported few autochthonous ZIKV infections. Acute-phase sera were tested from 77 patients who presented with a suspected arboviral illness from January to May 2018. Samples were tested for DENV and ZIKV RNA by real-time reverse transcription-PCR, and for DENV nonstructural protein 1 with a lateral-flow immunochromatographic test. Forty-one patients (51.2%) had acute dengue; no acute ZIKV infections were detected. Sixty-five patients (84.4%) had anti\u2013DENV-neutralizing antibodies by focus reduction neutralization testing (FRNT<jats:sub>50<\/jats:sub>). Qualitative detection with the pGOLD assay demonstrated good agreement with FRNT<jats:sub>50<\/jats:sub> (kappa = 0.74), and quantitative results were highly correlated between methods (<jats:italic>P<\/jats:italic> &lt; 0.001). Only three patients had anti\u2013ZIKV-neutralizing antibodies at titers of 1:55\u20131:80, and all three had corresponding DENV-neutralizing titers &gt; 1:4,000. Hospitalized dengue cases had significantly higher anti-DENV IgG levels (<jats:italic>P<\/jats:italic> &lt; 0.001). Anti-DENV IgG results from the pGOLD assay correlate well with FRNT, and quantitative results may inform patient risk stratification.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1449","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T15:32:46Z","timestamp":1617031966000},"page":"1729-1733","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Anti-Dengue and Anti-Zika IgG on a Plasmonic Gold Platform with Neutralization Testing"],"prefix":"10.4269","volume":"104","author":[{"given":"Alejandra","family":"Rojas","sequence":"first","affiliation":[{"name":"1Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muktha S.","family":"Natrajan","sequence":"additional","affiliation":[{"name":"2Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenna","family":"Weber","sequence":"additional","affiliation":[{"name":"3Department of Pathology, Stanford University School of Medicine, Palo Alto, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Cardozo","sequence":"additional","affiliation":[{"name":"4Departamento de Salud P\u00fablica, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"Cantero","sequence":"additional","affiliation":[{"name":"1Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeyarama S.","family":"Ananta","sequence":"additional","affiliation":[{"name":"5Nirmidas Biotech Inc., Palo Alto, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Kost","sequence":"additional","affiliation":[{"name":"5Nirmidas Biotech Inc., Palo Alto, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meijie","family":"Tang","sequence":"additional","affiliation":[{"name":"5Nirmidas Biotech Inc., Palo Alto, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanny","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"1Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Bernal","sequence":"additional","affiliation":[{"name":"1Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvalena","family":"Guill\u00e9n","sequence":"additional","affiliation":[{"name":"1Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mendoza","sequence":"additional","affiliation":[{"name":"4Departamento de Salud P\u00fablica, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malvina","family":"P\u00e1ez","sequence":"additional","affiliation":[{"name":"4Departamento de Salud P\u00fablica, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, San Lorenzo, Paraguay;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin A.","family":"Pinsky","sequence":"additional","affiliation":[{"name":"3Department of Pathology, Stanford University School of Medicine, Palo Alto, California;"},{"name":"6Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesse J.","family":"Waggoner","sequence":"additional","affiliation":[{"name":"2Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia;"},{"name":"7Department of Global Health, Rollins School of Public Health, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/5\/article-p1729.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/5\/article-p1729.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T20:41:53Z","timestamp":1629924113000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/5\/article-p1729.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,5]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1449","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,5]]}}}