{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:29:18Z","timestamp":1781886558141,"version":"3.54.5"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In the current genomic era, knowledge of diversity of <jats:italic>Leptospira<\/jats:italic>, the spirochetal agents of leptospirosis, is changing rapidly. Next-generation sequencing has decreased in price and increased in scale, with the potential to democratize large-scale analysis of pathogens in resource-limited, low\/middle-income (LMIC) regions. Consequently, the molecular classification of <jats:italic>Leptospira<\/jats:italic>, a pathogen disproportionately affecting LMIC countries, has changed dramatically over the last decade. <jats:italic>Leptospira<\/jats:italic> classification and molecular understandings of pathogen diversity have rapidly evolved, now most precisely based on core genome analysis supplemented by new insights provided by culture-independent methods directly using body fluids such as blood and urine. In places where leptospirosis disease burden is highest, genomic technologies have not been available, and serology-based methods remain the mainstay of leptospiral classification. Understanding the epidemiology, pathogenesis, and ultimately new approaches to treating and preventing leptospirosis requires detailed knowledge of regionally circulating <jats:italic>Leptospira<\/jats:italic> in highly endemic settings. Next-generation sequencing\u2013based, culture-independent typing overcomes the limitation of culture isolation of <jats:italic>Leptospira<\/jats:italic> from clinical samples, with promise of providing public health-actionable information applicable to leptospirosis-endemic LMIC settings.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1518","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T15:38:58Z","timestamp":1616427538000},"page":"1625-1627","source":"Crossref","is-referenced-by-count":5,"title":["Next-Generation Sequencing Analysis of Pathogenic Leptospira: A Way Forward for Understanding Infectious Disease Dynamics in Low\/Middle-Income, Disease-Endemic Settings"],"prefix":"10.4269","volume":"104","author":[{"given":"Suneth B.","family":"Agampodi","sequence":"first","affiliation":[{"name":"1Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut;"},{"name":"2Department of Community Medicine, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph M.","family":"Vinetz","sequence":"additional","affiliation":[{"name":"1Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/5\/article-p1625.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/5\/article-p1625.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T20:41:34Z","timestamp":1629924094000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/5\/article-p1625.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,5]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1518","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,5]]}}}