{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:02:52Z","timestamp":1780452172038,"version":"3.54.1"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,2]]},"abstract":"<jats:title>Abstract.<\/jats:title><jats:p><jats:italic>Strongyloides stercoralis<\/jats:italic> is a soil-transmitted nematode that can cause life-threatening conditions in immunocompromised persons. In the United States, strongyloidiasis should be considered mainly in immigrants, refugees, or travelers. The confirmatory laboratory diagnosis is usually performed by detecting larvae from the stool, duodenal material, and sputum. In persons who are immunocompromised with severe strongyloidiasis, adult worms and eggs can be detected from duodenal material. For serological diagnosis, most assays use crude antigens to detect anti-<jats:italic>S. stercoralis<\/jats:italic> IgG. Recently, recombinant proteins such as rSs-NIE-1 and rSs-IR have been used to detect IgG antibodies. We used rSs-NIE-1 and rSs-IR recombinant antigens to develop a biplex Western blot assay to detect the IgG4 antibody in individuals with strongyloidiasis. The sensitivities of rSs-NIE-1 and rSs-IR were 97.4% and 90.8%, respectively, whereas the specificities were 97.6% and 98%, respectively. In conclusion, the biplex rSs-NIE-1 and rSs-IR immunoblot performs well in detecting IgG4 antibody in <jats:italic>S. stercoralis-<\/jats:italic>infected persons.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1539","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T12:51:25Z","timestamp":1618836685000},"page":"2038-2041","source":"Crossref","is-referenced-by-count":9,"title":["Development of rSs-NIE-1 and rSs-IR Recombinant Antigen-Based Immunoblot for Detection of Antibody to Strongyloides stercoralis"],"prefix":"10.4269","volume":"104","author":[{"given":"Joelma Nascimento","family":"De Souza","sequence":"first","affiliation":[{"name":"1Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u2019Isha","family":"Langford","sequence":"additional","affiliation":[{"name":"2Kennesaw State University, Kennesaw, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"3Synergy, Co., Atlanta, Georgia;"},{"name":"4Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neci Matos","family":"Soares","sequence":"additional","affiliation":[{"name":"1Faculdade de Farm\u00e1cia, Universidade Federal da Bahia, Salvador, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukwan","family":"Handali","sequence":"additional","affiliation":[{"name":"4Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2038.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/104\/6\/article-p2038.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T00:42:06Z","timestamp":1628728926000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/104\/6\/article-p2038.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":0,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1539","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}