{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T09:39:42Z","timestamp":1775727582688,"version":"3.50.1"},"reference-count":0,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,2]]},"abstract":"<jats:title>ABSTRACT.<\/jats:title>\n<jats:p>Serological cross-reactivity has proved to be a challenge to diagnose Zika virus (ZIKV) infections in dengue virus (DENV) endemic countries. Confirmatory testing of ZIKV IgM positive results by plaque reduction neutralization tests (PRNTs) provides clarification in only a minority of cases because most individuals infected with ZIKV were previously exposed to DENV. The goal of this study was to evaluate the performance of a ZIKV\/DENV DUO IgM antibody capture ELISA (MAC-ELISA) for discriminating between DENV and ZIKV infections in endemic regions. Our performance evaluation included acute and convalescent specimens from patients with real-time reverse transcription polymerase chain reaction (RT-PCR)-confirmed DENV or ZIKV from the Sentinel Enhanced Dengue Surveillance System in Ponce, Puerto Rico. The ZIKV\/DENV DUO MAC-ELISA specificity was 100% for DENV (<jats:italic>N<\/jats:italic> = 127) and 98.4% for ZIKV (<jats:italic>N<\/jats:italic> = 275) when specimens were tested during the optimal testing window (days post-onset of illness [DPO] 6\u2013120). The ZIKV\/DENV DUO MAC-ELISA sensitivity of RT-PCR confirmed specimens reached 100% for DENV by DPO 6 and for ZIKV by DPO 9. Our new ZIKV\/DENV DUO MAC-ELISA was also able to distinguish ZIKV and DENV regardless of previous DENV exposure. We conclude this novel serologic diagnostic assay can accurately discriminate ZIKV and DENV infections. This can potentially be useful considering that the more labor-intensive and expensive PRNT assay may not be an option for confirmatory diagnosis in areas that lack PRNT capacity, but experience circulation of both DENV and ZIKV.<\/jats:p>","DOI":"10.4269\/ajtmh.20-1651","type":"journal-article","created":{"date-parts":[[2021,12,20]],"date-time":"2021-12-20T12:52:50Z","timestamp":1640004770000},"page":"585-592","source":"Crossref","is-referenced-by-count":15,"title":["Capacity of a Multiplex IgM Antibody Capture ELISA to Differentiate Zika and Dengue Virus Infections in Areas of Concurrent Endemic Transmission"],"prefix":"10.4269","volume":"106","author":[{"given":"Freddy A.","family":"Medina","sequence":"first","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances","family":"Vila","sequence":"additional","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmanane","family":"Premkumar","sequence":"additional","affiliation":[{"name":"2Department of Microbiology and Immunology University of North Carolina School of Medicine, Chapel Hill, North Carolina;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Lorenzi","sequence":"additional","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Paz-Bailey","sequence":"additional","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa I.","family":"Alvarado","sequence":"additional","affiliation":[{"name":"3Ponce Health Sciences University, Ponce, Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Rivera-Amill","sequence":"additional","affiliation":[{"name":"3Ponce Health Sciences University, Ponce, Puerto Rico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aravinda","family":"de Silva","sequence":"additional","affiliation":[{"name":"2Department of Microbiology and Immunology University of North Carolina School of Medicine, Chapel Hill, North Carolina;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Waterman","sequence":"additional","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Mu\u00f1oz-Jord\u00e1n","sequence":"additional","affiliation":[{"name":"1Surveillance and Research Laboratory, Dengue Branch, Centers for Disease Control and Prevention, San Juan, Puerto Rico;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/2\/article-p585.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/106\/2\/article-p585.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T14:58:59Z","timestamp":1643813939000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/106\/2\/article-p585.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,2]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.20-1651","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,2]]}}}