{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:06:11Z","timestamp":1791018371178,"version":"4.1.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5_suppl","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:p><jats:italic>Aotus<\/jats:italic> monkeys were used to determine the immunogenicity of Pvs25 protein expressed in the zygote\/ookinete surface. Animals were immunized in three times with 100 \u03bcg of Pvs25 formulated in Montanide ISA-720. Antibodies to Pvs25 detected by an enzyme-linked immunosorbent assay appeared by day 30 after the first immunization, with a peak of antibodies levels on day 150. These antibodies were still detectable on day 300. Plasma samples on day 150 from experimental group were able to completely block the development of the parasite in <jats:italic>Anopheles albimanus<\/jats:italic> mosquitoes artificially fed with human isolates of <jats:italic>Plasmodium vivax<\/jats:italic>. Immunized <jats:italic>Aotus<\/jats:italic> monkeys were infected with blood forms of the <jats:italic>P. vivax<\/jats:italic> Salvador I strain and no boosting effect of blood infection on titers of antibodies to Pvs25 was observed despite the presence of infective gametocytes. In conclusion, Pvs25 protein formulated in Montanide ISA-720 induces efficient and long-lasting transmission-blocking antibodies that cannot be boosted by parasite infection.<\/jats:p>","DOI":"10.4269\/ajtmh.2005.73.32","type":"journal-article","created":{"date-parts":[[2017,5,10]],"date-time":"2017-05-10T16:41:16Z","timestamp":1494434476000},"page":"32-37","source":"Crossref","is-referenced-by-count":37,"title":["INDUCTION OF TRANSMISSION-BLOCKING IMMUNITY IN AOTUS MONKEYS BY VACCINATION WITH A PLASMODIUM VIVAX CLINICAL GRADE PVS25 RECOMBINANT PROTEIN"],"prefix":"10.4269","volume":"73","author":[{"given":"MYRIAM","family":"ARE\u0301VALO-HERRERA","sequence":"first","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YEZID","family":"SOLARTE","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARI\u0301A FERNANDA","family":"YASNOT","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ANGE\u0301LICA","family":"CASTELLANOS","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ADRIANA","family":"RINCO\u0301N","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALLAN","family":"SAUL","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JIANBING","family":"MU","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CAROLE","family":"LONG","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LOUIS","family":"MILLER","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SO\u0301CRATES","family":"HERRERA","sequence":"additional","affiliation":[{"name":"Instituto de Inmunologi\u0301a del Valle, Universidad del Valle, Cali, Colombia; Malaria Vaccine and Drug Development Center, Cali, Colombia; Malaria Vaccine Development Branch and Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Am J Trop Med Hyg","first-page":"97","volume":"64","year":"2001"},{"key":"R2","series-title":"Trop Med Int Health","first-page":"836","volume":"1","year":"1996"},{"key":"R3","series-title":"Expert Opin Biol Ther","first-page":"619","volume":"1","year":"2001"},{"key":"R4","series-title":"Mol Biochem Parasitol","first-page":"59","volume":"61","year":"1993"},{"key":"R5","series-title":"Mol Biochem Parasitol","first-page":"161","volume":"78","year":"1996"},{"key":"R6","series-title":"Parasitol Int","first-page":"1","volume":"52","year":"2003"},{"key":"R7","series-title":"Infect Immun","first-page":"5576","volume":"62","year":"1994"},{"key":"R8","series-title":"J Exp Med","first-page":"1203","volume":"174","year":"1991"},{"key":"R9","series-title":"Infect Immun","first-page":"59","volume":"66","year":"1998"},{"key":"R10","series-title":"Mol Biochem Parasitol","first-page":"33","volume":"75","year":"1995"},{"key":"R11","series-title":"Parasite Immunol","first-page":"377","volume":"20","year":"1998"},{"key":"R12","series-title":"Vaccine","first-page":"763","volume":"20","year":"2001"},{"key":"R13","series-title":"Infect Immun","first-page":"6618","volume":"68","year":"2000"},{"key":"R14","series-title":"Vaccine","first-page":"3143","volume":"21","year":"2003"},{"key":"R15","series-title":"Vaccine","first-page":"3131","volume":"23","year":"2005"},{"key":"R16","series-title":"Mem Inst Oswaldo Cruz","first-page":"115","volume":"89","year":"1994"},{"key":"R17","series-title":"Ann Trop Med Parasitol","first-page":"49","volume":"91","year":"1997"},{"key":"R18","series-title":"Nature","first-page":"323","volume":"418","year":"2002"},{"key":"R19","series-title":"MMWR Surveill Summ","first-page":"1","volume":"52","year":"2003"},{"key":"R20","series-title":"Trans R Soc Trop Med Hyg","first-page":"15","volume":"73","year":"1979"},{"key":"R21","series-title":"Vaccine","first-page":"2504","volume":"18","year":"2000"},{"key":"R22","series-title":"Vaccine","first-page":"3145","volume":"17","year":"1999"},{"key":"R23","series-title":"Exp Parasitol","first-page":"229","volume":"71","year":"1990"},{"key":"R24","series-title":"Mol Biochem Parasitol","first-page":"147","volume":"110","year":"2000"},{"key":"R25","series-title":"Mol Biochem Parasitol","first-page":"155","volume":"20","year":"1986"},{"key":"R26","series-title":"Mol Biochem Parasitol","first-page":"263","volume":"59","year":"1993"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/73\/5_suppl\/article-p32.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/73\/5_suppl\/article-p32.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/73\/5_suppl\/article-p32.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T13:57:28Z","timestamp":1725371848000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/73\/5_suppl\/article-p32.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":26,"journal-issue":{"issue":"5_suppl"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2005.73.32","relation":{},"ISSN":["0002-9637"],"issn-type":[{"value":"0002-9637","type":"print"}],"subject":[],"published":{"date-parts":[[2005,11]]}}}