{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T16:17:40Z","timestamp":1781885860847,"version":"3.54.5"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p>There is an urgent need for new compounds for the drug development pipeline for treatment of patients with African sleeping sickness. One approach for identifying such compounds is by high throughput screening (HTS) of compound collections. For time and cost considerations, there is a need for the development of an assay that uses at least 384-well formats. To our knowledge, there are currently no viability assays for whole cell screening of trypanosomes in the 384-well plate format. We have developed and optimized an Alamar Blue viability assay in a 384-well format for <jats:italic>Trypanosoma brucei brucei<\/jats:italic> bloodstream form strain 427 (BS427). The assay had a Z\u2032 &gt; 0.5 and tolerated a final dimethyl-sulfoxide concentration of 0.42%. Drug sensitivity was compared with those reported from previously developed 96-well methods and was found to be comparable. The sensitivity and cost benefit of the Alamar Blue assay make it an excellent candidate for HTS application.<\/jats:p>","DOI":"10.4269\/ajtmh.2009.09-0015","type":"journal-article","created":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T17:32:47Z","timestamp":1255109567000},"page":"665-674","source":"Crossref","is-referenced-by-count":76,"title":["Development of an Alamar Blue\u2122 Viability Assay in 384-Well Format for High Throughput Whole Cell Screening of Trypanosoma brucei brucei Bloodstream Form Strain 427"],"prefix":"10.4269","volume":"81","author":[{"given":"Melissa L.","family":"Sykes","sequence":"first","affiliation":[{"name":"Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicky M.","family":"Avery","sequence":"additional","affiliation":[{"name":"Eskitis Institute for Cell and Molecular Therapies, Griffith University, Nathan, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Wkly Epidemiol Rec","first-page":"71","volume":"81","year":"2006"},{"key":"R2","series-title":"J Infect Dis","first-page":"1793","volume":"191","year":"2005"},{"key":"R3","series-title":"Trop Med Int Health","first-page":"906","volume":"6","year":"2001"},{"key":"R4","series-title":"Clin Infect Dis","first-page":"748","volume":"41","year":"2005"},{"key":"R5","series-title":"Nat Rev Drug Discov","first-page":"727","volume":"4","year":"2005"},{"key":"R6","series-title":"Chem Biol Drug Des","first-page":"355","volume":"67","year":"2006"},{"key":"R7","series-title":"Bioorg Med Chem Lett","first-page":"1280","volume":"17","year":"2007"},{"key":"R8","series-title":"Eur J Biochem","first-page":"5421","volume":"267","year":"2000"},{"key":"R9","series-title":"Exp Parasitol","first-page":"403","volume":"73","year":"1991"},{"key":"R10","series-title":"Acta Trop","first-page":"139","volume":"68","year":"1997"},{"key":"R11","series-title":"Mol Pharmacol","first-page":"1585","volume":"70","year":"2006"},{"key":"R12","series-title":"Antimicrob Agents Chemother","first-page":"5169","volume":"49","year":"2005"},{"key":"R13","series-title":"Kinetoplastid Biol Dis","first-page":"3","volume":"5","year":"2006"},{"key":"R14","series-title":"Proc Natl Acad Sci USA","first-page":"3450","volume":"75","year":"1978"},{"key":"R15","series-title":"J Protozool","first-page":"349","volume":"23","year":"1976"},{"key":"R16","series-title":"J Ethnopharmacol","first-page":"37","volume":"91","year":"2004"},{"key":"R17","series-title":"J Med Chem","first-page":"3440","volume":"44","year":"2001"},{"key":"R18","series-title":"J Biomol Screen","first-page":"67","volume":"4","year":"1999"},{"key":"R19","series-title":"J Biomol Screen","first-page":"247","volume":"11","year":"2006"},{"key":"R20","series-title":"J Biomol Screen","first-page":"229","volume":"12","year":"2007"},{"key":"R21","series-title":"J Parasitol","first-page":"985","volume":"75","year":"1989"},{"key":"R22","year":"2001"},{"key":"R23","series-title":"Mol Cell Biol","first-page":"1137","volume":"18","year":"1998"},{"key":"R24","series-title":"Trends Biotechnol","first-page":"105","volume":"24","year":"2006"},{"key":"R25","series-title":"Toxicol In Vitro","first-page":"559","volume":"8","year":"1994"},{"key":"R26","series-title":"Toxicol In Vitro","first-page":"703","volume":"18","year":"2004"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/4\/article-p665.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/4\/article-p665.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/4\/article-p665.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T14:48:10Z","timestamp":1725288490000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/4\/article-p665.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":26,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2009.09-0015","relation":{},"ISSN":["0002-9637"],"issn-type":[{"value":"0002-9637","type":"print"}],"subject":[],"published":{"date-parts":[[2009,10]]}}}