{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:26:20Z","timestamp":1761128780908},"reference-count":27,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>Infection with Japanese encephalitis virus (JEV) is a major public health problem in Asia. Detection of JEV-specific IgM in serum and cerebrospinal fluid (CSF) by the IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) is currently the most widely used diagnostic method to detect JEV infection. Because of the possible presence of IgM cross-reactivity with other flaviviruses in serum and the high ratio of inapparent-to-apparent JEV infections, a positive result in serum only suggests a recent infection and not necessarily an encephalitic illness caused by JEV. Consequently, detection of JEV-specific IgM in CSF assumes great diagnostic relevance. We evaluated two commercial JEV MAC-ELISA kits using 60 CSF samples obtained from patients with acute encephalitis syndrome. The Panbio and XCyton kits had sensitivities of 65\u201380% and 95% and specificities of 90% and 97.5%, respectively. Performance information on these commercial JEV MAC-ELISA kits for CSF should assist in laboratory-based JE surveillance programs.<\/jats:p>","DOI":"10.4269\/ajtmh.2009.09-0144","type":"journal-article","created":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T22:29:10Z","timestamp":1260311350000},"page":"1144-1150","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation of IgM Antibody Capture Enzyme-Linked Immunosorbent Assay Kits for Detection of IgM against Japanese Encephalitis Virus in Cerebrospinal Fluid Samples"],"prefix":"10.4269","volume":"81","author":[{"given":"Vasanthapuram","family":"Ravi","sequence":"first","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaimie S.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandy J.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Desai","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nalini","family":"Ramamurty","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Featherstone","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara W.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Neurovirology, National Institute of Mental Health and Neuro Sciences, Bangalore, India; Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado; Immunization and Vaccine Development, World Health Organization South East Asia Regional Office, New Delhi, India; Department of Immunization, Vaccines, and Biologicals, World Health Organization, Geneva, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"year":"2000","key":"R1"},{"key":"R2","series-title":"Bull World Health Organ","first-page":"178","volume":"86","year":"2008"},{"year":"2006","key":"R3"},{"key":"R4","series-title":"J Neurol Neurosurg Psychiatry","first-page":"405","volume":"68","year":"2000"},{"key":"R5","series-title":"Vaccine","first-page":"1","volume":"18","year":"2000"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"164","volume":"77","year":"2007"},{"key":"R7","series-title":"J Infect Dis","first-page":"1093","volume":"151","year":"1985"},{"key":"R8","series-title":"Vaccine","first-page":"2259","volume":"17","year":"1999"},{"key":"R9","series-title":"J Clin Virol","first-page":"429","volume":"35","year":"2006"},{"key":"R10","series-title":"Indian J Med Res","first-page":"198","volume":"99","year":"1994"},{"key":"R11","series-title":"Brain Dev","first-page":"447","volume":"28","year":"2006"},{"key":"R12","series-title":"Clin Diagn Lab Immunol","first-page":"544","volume":"9","year":"2002"},{"key":"R13","series-title":"J Clin Microbiol","first-page":"1823","volume":"38","year":"2000"},{"key":"R14","series-title":"Virology","first-page":"239","volume":"333","year":"2005"},{"key":"R15","series-title":"Virology","first-page":"170","volume":"306","year":"2003"},{"key":"R16","series-title":"J Clin Microbiol","first-page":"3227","volume":"43","year":"2005"},{"key":"R17","series-title":"J Virol Methods","first-page":"62","volume":"145","year":"2007"},{"year":"2007","key":"R18"},{"year":"1995","key":"R19"},{"year":"2007","key":"R20"},{"key":"R21","series-title":"Clin Vaccine Immunol","first-page":"1052","volume":"16","year":"2009"},{"key":"R22","series-title":"J Virol","first-page":"3095","volume":"73","year":"1999"},{"key":"R23","series-title":"J Virol","first-page":"7290","volume":"75","year":"2001"},{"key":"R24","series-title":"Am J Trop Med Hyg","first-page":"639","volume":"71","year":"2004"},{"key":"R25","series-title":"J Gen Virol","first-page":"37","volume":"70","year":"1989"},{"key":"R26","series-title":"Clin Diagn Lab Immunol","first-page":"1130","volume":"11","year":"2004"},{"key":"R27","series-title":"Viral Immunol","first-page":"469","volume":"13","year":"2000"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/6\/article-p1144.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/6\/article-p1144.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/6\/article-p1144.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T14:52:20Z","timestamp":1725288740000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/6\/article-p1144.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":27,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2009.09-0144","relation":{},"ISSN":["0002-9637"],"issn-type":[{"type":"print","value":"0002-9637"}],"subject":[],"published":{"date-parts":[[2009,12]]}}}