{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T05:04:23Z","timestamp":1773983063374,"version":"3.50.1"},"reference-count":28,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p>We have applied a loop-mediated isothermal amplification (LAMP) technique to detect <jats:italic>Leishmania donovani<\/jats:italic> DNA. The LAMP technique detected 1 fg of <jats:italic>L. donovani<\/jats:italic> DNA, which was 10-fold more sensitive than a conventional polymerase chain reaction (PCR). All nested PCR\u2013positive blood samples from visceral leishmaniasis patients were positive with the LAMP technique, and DNA samples from <jats:italic>L. infantum<\/jats:italic>, <jats:italic>L. major<\/jats:italic>, <jats:italic>L. mexicana<\/jats:italic>, <jats:italic>L. tropica<\/jats:italic>, <jats:italic>L. braziliensis<\/jats:italic>, <jats:italic>Plasmodium falciparum<\/jats:italic>, and healthy humans were negative with the LAMP technique. The advantages of the LAMP method are its shorter reaction time, a lack of requirement of sophisticated equipment, and visual judgment of positivity based on the turbidity of reaction mixture. Our LAMP technique can be a better alternative to a conventional PCR, especially under field conditions.<\/jats:p>","DOI":"10.4269\/ajtmh.2009.09-0145","type":"journal-article","created":{"date-parts":[[2009,10,9]],"date-time":"2009-10-09T17:32:47Z","timestamp":1255109567000},"page":"578-582","source":"Crossref","is-referenced-by-count":66,"title":["Sensitive, Specific, and Rapid Detection of Leishmania donovani DNA by Loop-Mediated Isothermal Amplification"],"prefix":"10.4269","volume":"81","author":[{"given":"Hidekazu","family":"Takagi","sequence":"first","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Zahidul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdur","family":"Razzaque","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R. M. Saifuddin","family":"Ekram","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihisa","family":"Hashighuchi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; Department of Medicine, Rajshahi Medical College Hospital, Rajshahi, Bangladesh; Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan; Department of Nephrology and Endocrinology, and Department of Hemodialysis and Apheresis, University Hospital, The University of Tokyo, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eisei","family":"Noiri","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan; 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