{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:24:55Z","timestamp":1791156295544,"version":"4.1.0"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p>Real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) supplemented with melting curve analysis is a highly sensitive and fast method offering a high throughput. We report the development of a real-time FRET PCR for molecular detection of <jats:italic>Opisthorchis viverrini<\/jats:italic> in human fecal samples. The diagnostic sensitivity, specificity, accuracy, and positive and negative predictive values of this method were 97.5%, 100%, 98.9%, 100%, and 98.2%, respectively. The sensitivity was not significantly different from that of the quantified formalin-ethyl acetate concentration technique, the gold standard (<jats:italic>P<\/jats:italic> &gt; 0.05). The procedure has potential for diagnosis of human opisthorchiasis in disease-endemic areas, for large epidemiologic investigations involving at risk populations, and monitoring eradication programs of the liver fluke, which causes hepatobiliary diseases and induces cholangiocarcinoma.<\/jats:p>","DOI":"10.4269\/ajtmh.2009.09-0275","type":"journal-article","created":{"date-parts":[[2009,10,27]],"date-time":"2009-10-27T19:03:21Z","timestamp":1256670201000},"page":"917-920","source":"Crossref","is-referenced-by-count":19,"title":["Rapid Molecular Detection of Opisthorchis viverrini in Human Fecal Samples by Real-Time Polymerase Chain Reaction"],"prefix":"10.4269","volume":"81","author":[{"given":"Pewpan M.","family":"Intapan","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viraphong","family":"Lulitanond","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phunthira","family":"Pongsaskulchoti","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand; Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"World Health Organ Tech Rep Ser","first-page":"1","volume":"849","year":"1995"},{"key":"R2","series-title":"Int J Parasitol","first-page":"1233","volume":"35","year":"2005"},{"key":"R3","series-title":"Emerg Infect Dis","first-page":"2122","volume":"10","year":"2004"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"1279","volume":"33","year":"1984"},{"key":"R5","series-title":"Am J Gastroenterol","first-page":"76","volume":"81","year":"1986"},{"key":"R6","series-title":"J Helminthol","first-page":"102","volume":"67","year":"1993"},{"key":"R7","series-title":"Trans R Soc Trop Med Hyg","first-page":"194","volume":"87","year":"1993"},{"key":"R8","series-title":"Trop Geogr Med","first-page":"95","volume":"32","year":"1980"},{"key":"R9","series-title":"Arzneimittelforschung","first-page":"1167","volume":"34","year":"1984"},{"key":"R10","series-title":"Trans R Soc Trop Med Hyg","first-page":"561","volume":"88","year":"1994"},{"key":"R11","series-title":"PLoS Med","first-page":"1148","volume":"4","year":"2007"},{"key":"R12","series-title":"Parasitology","first-page":"175","volume":"122","year":"2001"},{"key":"R13","series-title":"J Clin Microbiol","first-page":"3879","volume":"40","year":"2002"},{"key":"R14","series-title":"Acta Trop","first-page":"26","volume":"97","year":"2006"},{"key":"R15","series-title":"J Clin Microbiol","first-page":"366","volume":"46","year":"2008"},{"key":"R16","series-title":"Exp Parasitol","first-page":"353","volume":"120","year":"2008"},{"key":"R17","series-title":"Parasitol Res","first-page":"649","volume":"103","year":"2008"},{"key":"R18","series-title":"Vet Parasitol","first-page":"65","volume":"157","year":"2008"},{"key":"R19","series-title":"Exp Parasitol","first-page":"217","volume":"119","year":"2008"},{"key":"R20","series-title":"J Mol Diagn","first-page":"93","volume":"11","year":"2009"},{"key":"R21","series-title":"Trans R Soc Trop Med Hyg","first-page":"774","volume":"80","year":"1986"},{"key":"R22","series-title":"Mol Cell Probes","first-page":"399","volume":"5","year":"1991"},{"key":"R23","series-title":"Am J Trop Med Hyg","first-page":"509","volume":"78","year":"2008"},{"key":"R24","series-title":"Exp Parasitol","first-page":"67","volume":"113","year":"2006"},{"key":"R25","series-title":"Pediatr Clin North Am","first-page":"861","volume":"27","year":"1980"},{"key":"R26","series-title":"Am J Trop Med Hyg","first-page":"521","volume":"52","year":"1995"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/5\/article-p917.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/5\/article-p917.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/5\/article-p917.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T14:49:14Z","timestamp":1725288554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/5\/article-p917.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11]]},"references-count":26,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2009.09-0275","relation":{},"ISSN":["0002-9637"],"issn-type":[{"value":"0002-9637","type":"print"}],"subject":[],"published":{"date-parts":[[2009,11]]}}}