{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:20:55Z","timestamp":1791091255642,"version":"4.1.0"},"reference-count":12,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Trop Med Hyg"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>Forty-two patients with visceral leishmaniasis in Tunisia were treated with meglumine antimoniate and followed-up for clinical improvement and blood parasite load determined by quantitative real-time polymerase chain reaction (PCR). Parasite loads before treatment ranged from 27 to 5.3 \u00d7 10<jats:sup>7<\/jats:sup> parasites\/mL. At the end of treatment, parasite load decreased significantly in 39 cured patients (<jats:italic>P<\/jats:italic> &lt; 0.001). The decrease in parasite load after treatment was greater than 99% for 34 patients and PCR results became negative in 23 of them. Two patients without clinical improvement showed no or slight decreases in parasite load (209 versus 202 parasites\/mL and 1,765 versus 146 parasites\/mL). One patient showed had a relapse seven months after showing a good response to treatment. His parasitemia remained high despite a sharp decrease (5.2 \u00d7 10<jats:sup>5<\/jats:sup> versus 5.9 \u00d7 10<jats:sup>3<\/jats:sup> parasites\/mL).<\/jats:p>","DOI":"10.4269\/ajtmh.2009.09-0285","type":"journal-article","created":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T22:29:10Z","timestamp":1260311350000},"page":"1004-1006","source":"Crossref","is-referenced-by-count":16,"title":["Contribution of Quantitative Real-Time Polymerase Chain Reaction to Follow-Up of Visceral Leishmaniasis Patients Treated with Meglumine Antimoniate"],"prefix":"10.4269","volume":"81","author":[{"given":"Karim","family":"Aoun","sequence":"first","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emira","family":"Chouihi","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fethi","family":"Amri","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nissaf Ben","family":"Alaya","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Raies","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Mary","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Bouratbine","sequence":"additional","affiliation":[{"name":"Research Laboratory of Emerging Parasitic Diseases, Tunis, Tunisia; Laboratory of Parasitology and Mycology, and Department of Medical Epidemiology and Statistics, Pasteur Institute, Tunis, Tunisia; Department of Paediatrics, Kairouan, Tunisia; Laboratory of Micro-organisms and Active Bio-molecules, University of Sciences, Tunis, Tunisia; Laboratory of Parasitology and Mycology, La Timone Hospital, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Tunis Med","first-page":"535","volume":"81","year":"2003"},{"key":"R2","series-title":"Med Mal Infect","first-page":"446","volume":"28","year":"1998"},{"key":"R3","year":"2008"},{"key":"R4","series-title":"J Clin Microbiol","first-page":"5249","volume":"42","year":"2004"},{"key":"R5","series-title":"Am J Trop Med Hyg","first-page":"46","volume":"81","year":"2009"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"858","volume":"75","year":"2006"},{"key":"R7","series-title":"Ann Parasitol Hum Comp","first-page":"111","volume":"65","year":"1990"},{"key":"R8","series-title":"Med Mal Infect","first-page":"74","volume":"35","year":"2005"},{"key":"R9","series-title":"Pediatrics","first-page":"E27","volume":"109","year":"2002"},{"key":"R10","series-title":"J Clin Microbiol","first-page":"2343","volume":"44","year":"2006"},{"key":"R11","series-title":"Trans R Soc Trop Med Hyg","first-page":"927","volume":"81","year":"1987"},{"key":"R12","series-title":"Antimicrob Agents Chemother","first-page":"827","volume":"41","year":"1997"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/6\/article-p1004.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/6\/article-p1004.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/81\/6\/article-p1004.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T14:52:32Z","timestamp":1725288752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/81\/6\/article-p1004.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":12,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2009.09-0285","relation":{},"ISSN":["0002-9637"],"issn-type":[{"value":"0002-9637","type":"print"}],"subject":[],"published":{"date-parts":[[2009,12]]}}}