{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T12:46:08Z","timestamp":1759063568661},"reference-count":11,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>An automated colorimetric micro-neutralization assay (CmNt) was developed for confirmation and differentiation of West Nile Virus (WNV)-positive human sera as a higher throughput alternative to the standard six-well plaque-reduction neutralization test (PRNT). CmNt was performed in high-capacity 96-well micro-titer plates and required 4\u20136 days to complete. Inhibition of infection was determined by reduced neutral red-dye retention and conveniently recorded by a colorimetric plate reader. Human sera previously confirmed by PRNT as either negative (<jats:italic>N<\/jats:italic> = 52), WNV positive (<jats:italic>N<\/jats:italic> = 81), or St. Louis encephalitis virus positive (<jats:italic>N<\/jats:italic> = 12) were tested by CmNt; interpreted results were virtually identical to PRNT with a reduced turnaround time and higher throughput. Additionally, a handful of dengue virus positive and negative specimens (four each) were tested by CmNt; interpreted results were identical to PRNT.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0297","type":"journal-article","created":{"date-parts":[[2010,3,5]],"date-time":"2010-03-05T18:59:46Z","timestamp":1267815586000},"page":"501-504","source":"Crossref","is-referenced-by-count":17,"title":["High Throughput Quantitative Colorimetric Microneutralization Assay for the Confirmation and Differentiation of West Nile Virus and St. Louis Encephalitis Virus"],"prefix":"10.4269","volume":"82","author":[{"given":"Michael","family":"Taketa-Graham","sequence":"first","affiliation":[{"name":"California Department of Public Health, Viral and Rickettsial Disease Laboratory, Center for Infectious Disease, Richmond, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime L.","family":"Powell Pereira","sequence":"additional","affiliation":[{"name":"California Department of Public Health, Viral and Rickettsial Disease Laboratory, Center for Infectious Disease, Richmond, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Baylis","sequence":"additional","affiliation":[{"name":"California Department of Public Health, Viral and Rickettsial Disease Laboratory, Center for Infectious Disease, Richmond, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Cossen","sequence":"additional","affiliation":[{"name":"California Department of Public Health, Viral and Rickettsial Disease Laboratory, Center for Infectious Disease, Richmond, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leopoldo","family":"Oceguera","sequence":"additional","affiliation":[{"name":"California Department of Public Health, Viral and Rickettsial Disease Laboratory, Center for Infectious Disease, Richmond, 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