{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:27:48Z","timestamp":1789846068643,"version":"4.0.1"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:p>People cured from visceral leishmaniasis (VL) develop protection mediated by Th1-type cellular responses against new infections. We evaluated cytokine responses against 6 defined candidate vaccine antigens in 15 cured VL subjects and 5 healthy endemic controls with no evidence of previous exposure to <jats:italic>Leishmania<\/jats:italic> parasites. Of the 6 cytokines examined, only interferon-gamma (IFN-\u03b3) differentiated cured VL patients from non-exposed individuals, with cured patients mounting a significantly higher IFN-\u03b3 response to a crude parasite antigen preparation. Among candidate vaccine antigens tested, the largest number of cured subjects recognized cysteine proteinase B, leading to heightened IFN-\u03b3 responses, followed by sterol 24-c-methyltransferase. These two antigens were the most immunogenic and protective antigens in a murine VL model, indicating a relationship between T cell recall responses of humans cured from VL and protective efficacy in an experimental model. Further studies may help prioritize antigens for clinical development of a subunit vaccine against VL.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0341","type":"journal-article","created":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T01:02:37Z","timestamp":1272934957000},"page":"808-813","source":"Crossref","is-referenced-by-count":31,"title":["Evaluation of Ex Vivo Human Immune Response against Candidate Antigens for a Visceral Leishmaniasis Vaccine"],"prefix":"10.4269","volume":"82","author":[{"given":"Rajiv","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyuki","family":"Goto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamlesh","family":"Gidwani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen 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