{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:57:17Z","timestamp":1781632637672,"version":"3.54.5"},"reference-count":26,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,2]]},"abstract":"Monkeypox virus (MPXV), a member of the family Poxviridae<\/jats:italic> and genus Orthopoxvirus<\/jats:italic>, causes a smallpox-like disease in humans. A previously described pan-Orthopoxvirus<\/jats:italic> assay, based on a broad-range polymerase chain reaction (PCR) coupled with electrospray ionization mass spectrometry (PCR\/ESI-MS), was evaluated for its ability to detect MPXV from spiked human and aerosol-infected cynomolgous macaque (Macaca fascicularis<\/jats:italic>) samples. Detection of MPXV DNA from macaque tissue, blood, and spiked human blood by the PCR\/ESI-MS pan-Orthopoxvirus<\/jats:italic> assay was comparable, albeit at slightly higher levels, to the current gold standard method of real-time PCR with the pan-Orthopoxvirus<\/jats:italic> assay and had a limit of detection of 200 plaque-forming units. Furthermore, the platform was able to distinguish MPXV and vaccinia viruses that were spiked into macaque blood samples at various concentrations. This platform provides a new tool for the diagnosis and monitoring of orthopoxviral loads during vaccine or antiviral studies, but also could provide rapid identification during natural outbreaks or bioterrorism attacks.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0361","type":"journal-article","created":{"date-parts":[[2010,2,5]],"date-time":"2010-02-05T01:22:23Z","timestamp":1265332943000},"page":"318-323","source":"Crossref","is-referenced-by-count":24,"title":["Application of the Ibis-T5000 Pan-Orthopoxvirus Assay to Quantitatively Detect Monkeypox Viral Loads in Clinical Specimens from Macaques Experimentally Infected with Aerosolized Monkeypox Virus"],"prefix":"10.4269","volume":"82","author":[{"given":"Rebecca J.","family":"Grant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carson 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Republic of Congo, 1996 to 1997","volume":"7","author":"Hutin","year":"2001"},{"key":"R6","series-title":"Acat Path Microbiol Scand","first-page":"156","article-title":"A pox-like disease in cynomolgus monkeys","volume":"46","author":"Magnus","year":"1959"},{"key":"R7","series-title":"Lab Invest","first-page":"1200","article-title":"Monkeypox virus detection in rodents using real-time 3\u2032-minor groove binder TaqMan assays on the Roche LightCycler","volume":"84","author":"Kulesh","year":"2004"},{"key":"R8","series-title":"N Engl J Med","first-page":"342","article-title":"The detection of monkeypox in humans in the Western Hemisphere","volume":"350","author":"Reed","year":"2004"},{"key":"R9","series-title":"Acat Path Microbiol Scand","first-page":"156","article-title":"A pox-like disease in cynomolgus monkeys","volume":"46","author":"Magnus","year":"1959"},{"key":"R10","series-title":"Virology","first-page":"46","article-title":"Virulence differences between monkeypox virus isolates from west Africa and the Congo basin","volume":"340","author":"Chen","year":"2005"},{"key":"R11","series-title":"J Clin Virol","first-page":"194","article-title":"Detection of monkeypox virus with real-time PCR assays","volume":"36","author":"Li","year":"2006"},{"key":"R12","series-title":"Am J Trop Med Hyg","first-page":"768","article-title":"Efficacy of the antipoxvirus compound ST-246 for treatment of severe orthopoxvirus infection","volume":"76","author":"Sbrana","year":"2007"},{"key":"R13","series-title":"Nature","first-page":"745","article-title":"Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infection","volume":"439","author":"Stittelaar","year":"2006"},{"key":"R14","series-title":"J Virol","first-page":"7845","article-title":"Modified vaccinia virus Ankara protects macaques against respiratory challenge with monkeypox virus","volume":"79","author":"Stittelaar","year":"2005"},{"key":"R15","series-title":"Antiviral 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spectrometry","volume":"2","author":"Sampath","year":"2007"},{"key":"R20","series-title":"Virology","first-page":"286","article-title":"Direct broad-range detection of alphaviruses in mosquito extracts","volume":"368","author":"Eshoo","year":"2007"},{"key":"R21","series-title":"Emerg Infect Dis","first-page":"373","article-title":"Rapid identification of emerging pathogens: coronavirus","volume":"11","author":"Sampath","year":"2005"},{"key":"R22","series-title":"PloS One","first-page":"e6342","article-title":"Rapid and high-throughput pan-Orthopoxvirus detection and identification by broad-range PCR and mass spectrometry","volume":"4","author":"Eshoo","year":"2009"},{"key":"R23","series-title":"J Gen Virol","first-page":"1975","article-title":"Sequence alterations within and downstream of the A-type inclusion protein genes allow differentiation of Orthopoxvirus species by polymerase chain reaction","volume":"75","author":"Meyer","year":"1994"},{"key":"R24","series-title":"J Clin 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