{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:18:32Z","timestamp":1793312312841,"version":"4.5.0"},"reference-count":29,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:p>Here we describe a generic, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay, for the identification of <jats:italic>Leishmania<\/jats:italic> species from clinical samples. LAMP is an isothermal reaction recently developed as a point-of-care diagnostic tool. Primers were designed in the conserved region of the 18S ribosomal RNA (rRNA) gene; amplification was visualized by the pre-amplification addition of fluorescent detection reagent (FDR) and a simple UV lamp. By using a reverse-transcriptase step, the system detected infections between 10 and 100 parasites per mL. The assay was tested on a range of nucleic acid extracts from <jats:italic>Leishmania<\/jats:italic> species, visceral leishmaniasis (VL) patients from Sudan, and cutaneous leishmaniasis (CL) patients from Suriname. The sensitivity of RT-LAMP from the blood of VL patients was 83% (<jats:italic>N<\/jats:italic> = 30) compared with microscopy of bone-marrow and lymph-node aspirates; for CL patients the observed sensitivity was 98% (<jats:italic>N<\/jats:italic> = 43). The potential to use LAMP as a diagnostic tool for leishmaniasis is discussed.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0369","type":"journal-article","created":{"date-parts":[[2010,3,26]],"date-time":"2010-03-26T23:33:07Z","timestamp":1269646387000},"page":"591-596","source":"Crossref","is-referenced-by-count":95,"title":["Development of a Reverse Transcriptase Loop-Mediated Isothermal Amplification (LAMP) Assay for the Sensitive Detection of Leishmania Parasites in Clinical Samples"],"prefix":"10.4269","volume":"82","author":[{"given":"Emily R.","family":"Adams","sequence":"first","affiliation":[{"name":"Koninklijk Instituut voor de Tropen (KIT) Biomedical Research, Amsterdam, The Netherlands; Faculty of Medicine, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard J.","family":"Schoone","sequence":"additional","affiliation":[{"name":"Koninklijk Instituut voor de Tropen (KIT) Biomedical Research, Amsterdam, The Netherlands; Faculty of Medicine, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al Farazdag","family":"Ageed","sequence":"additional","affiliation":[{"name":"Koninklijk Instituut voor de Tropen (KIT) Biomedical Research, Amsterdam, The Netherlands; Faculty of Medicine, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayda El","family":"Safi","sequence":"additional","affiliation":[{"name":"Koninklijk Instituut voor de Tropen (KIT) Biomedical Research, Amsterdam, The Netherlands; Faculty of Medicine, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk D. F. H.","family":"Schallig","sequence":"additional","affiliation":[{"name":"Koninklijk Instituut voor de Tropen (KIT) Biomedical Research, Amsterdam, The Netherlands; Faculty of Medicine, University of Khartoum, Khartoum, Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Infect Dis","first-page":"1","article-title":"Clinical spectrum of leishmaniasis","volume":"22","author":"Pearson","year":"1996"},{"key":"R2","series-title":"Lancet Infect Dis","first-page":"87","article-title":"Post-kala-azar dermal leishmaniasis","volume":"3","author":"Zijlstra","year":"2003"},{"key":"R3","series-title":"Indian J Med Res","first-page":"295","article-title":"Challenges in the diagnosis of post kala-azar dermal leishmaniasis","volume":"123","author":"Salotra","year":"2006"},{"key":"R4","series-title":"Comp Immunol Microbiol Infect Dis","first-page":"305","article-title":"Leishmaniasis: current situation and new perspectives","volume":"27","author":"Desjeux","year":"2004"},{"key":"R5","series-title":"Clin Infect Dis","first-page":"684","article-title":"Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years","volume":"24","author":"Berman","year":"1997"},{"key":"R6","series-title":"J Am Acad Dermatol","first-page":"70","article-title":"Value of diagnostic techniques for cutaneous leishmaniasis","volume":"49","author":"Faber","year":"2003"},{"issue":"(Suppl 1)","key":"R7","series-title":"Trans R Soc Trop Med Hyg","first-page":"S27","article-title":"Leishmaniasis in Sudan. 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