{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:23:46Z","timestamp":1790555026236,"version":"4.1.0"},"reference-count":25,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>The purpose of this study was to perform a 16S sequence-based quality control of two <jats:italic>Leptospira<\/jats:italic> strain collections. 16S rRNA gene sequencing was used to verify two <jats:italic>Leptospira<\/jats:italic> reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify <jats:italic>Leptospira<\/jats:italic> beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of <jats:italic>Leptospira<\/jats:italic> reference collections.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0558","type":"journal-article","created":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T16:47:50Z","timestamp":1263228470000},"page":"83-87","source":"Crossref","is-referenced-by-count":22,"title":["Monitoring Leptospira Strain Collections: The Need for Quality Control"],"prefix":"10.4269","volume":"82","author":[{"given":"Gustavo M.","family":"Cerqueira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan J. 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