{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T06:05:43Z","timestamp":1744178743843},"reference-count":12,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>Profiles of <jats:italic>Burkholderia pseudomallei<\/jats:italic> persistence and antibodies in blood, spleen, liver, and lungs of infected BALB\/c mice were investigated. Animals were infected intraperitoneally with low (6 colony-forming units) or high (230 colony-forming units) doses of <jats:italic>B. pseudomallei<\/jats:italic>. In the high-dose infected group, bacteria were found by culture in 100% of blood, liver, spleen and lung samples at 24 and 48 hours after infections; blood samples were 100% positive by polymerase chain reaction after 60 hours. Antibody responses in the high-dose infected group were low. These responses were detected in the low-dose infected group after 5 days, peaked at 7\u201314 days, and showed persistence until 28 days post-infection. Bacterial loads and antibody profiles varied according to the level of bacterial infections. This kinetic study, although in animals, provides crucial knowledge that might be useful for the development of a sensitive and specific diagnostic assay for patients with melioidosis.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0567","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T19:04:52Z","timestamp":1275505492000},"page":"1102-1105","source":"Crossref","is-referenced-by-count":5,"title":["Bacterial Loads and Antibody Responses in BALB\/c Mice Infected with Low and High Doses of Burkholderia pseudomallei"],"prefix":"10.4269","volume":"82","author":[{"given":"Nuttiya","family":"Srisurat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasana W.","family":"Sermswan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Unchalee","family":"Tatawasart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surasakdi","family":"Wongratanacheewin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Microbiol Rev","first-page":"383","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005"},{"key":"R2","series-title":"J Infect Dis","first-page":"890","article-title":"Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand","volume":"159","author":"Chaowagul","year":"1989"},{"key":"R3","series-title":"Lancet","first-page":"1715","article-title":"Melioidosis","volume":"361","author":"White","year":"2003"},{"key":"R4","series-title":"Acta Trop","first-page":"235","article-title":"Recent developments in laboratory diagnosis of melioidosis","volume":"74","author":"Sirisinha","year":"2000"},{"key":"R5","series-title":"Acta Trop","first-page":"247","article-title":"Comparison of three PCR primer sets for diagnosis of septicemic melioidosis","volume":"74","author":"Kunakorn","year":"2000"},{"key":"R6","series-title":"J Med Microbiol","first-page":"1075","article-title":"Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia","volume":"49","author":"Anuntagool","year":"2000"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"146","article-title":"Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis","volume":"63","author":"Sermswan","year":"2000"},{"key":"R8","series-title":"Infect Immun","first-page":"1106","article-title":"The capsular polysaccharide of Burkholderia pseudomallei contributes to survival in serum by reducing complement factor C3b deposition","volume":"73","author":"Reckseidler-Zenteno","year":"2005"},{"key":"R9","series-title":"Mol Cell Probes","first-page":"25","article-title":"Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction","volume":"11","author":"Rattanathongkom","year":"1997"},{"year":"2001","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","key":"R10"},{"key":"R11","series-title":"BMC Genomics","first-page":"174","article-title":"Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies","volume":"6","author":"Kim","year":"2005"},{"key":"R12","series-title":"Infect Immun","first-page":"4494","article-title":"Immunostimulatory CpG oligodeoxynucleotide confers protection in a murine model of infection with Burkholderia pseudomallei","volume":"72","author":"Wongratanacheewin","year":"2004"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/82\/6\/article-p1102.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/82\/6\/article-p1102.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/82\/6\/article-p1102.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T15:58:58Z","timestamp":1725292738000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/82\/6\/article-p1102.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":12,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0567","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}