{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:23:54Z","timestamp":1789590234786,"version":"build-2803163510"},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:p>\n                    Scrub typhus is a rickettsial disease transmitted to humans through the bite of chigger mites infected with\n                    <jats:italic>Orientia tsutsugamushi<\/jats:italic>\n                    , and is an endemic disease in Taiwan. To elucidate the molecular epidemiology of\n                    <jats:italic>O. tsutsugamushi<\/jats:italic>\n                    , the complete open reading frame of the 56-kDa type-specific antigen gene sequence of strains isolated from scrub typhus patients were determined and analyzed. A total of 116 isolates of\n                    <jats:italic>O. tsutsugamushi<\/jats:italic>\n                    were successfully isolated from patients infected in diverse geographic origins including Taiwan and three offshore islets, Kinmen, Matsu, and Penghu between May 2006 and December 2007. Sequence analysis revealed that 22 distinct sequence types could be identified that were broadly distributed in different clusters of the phylogenetic tree. Most of the isolates belong to Karp, Kawasaki, and Kuroki genotypes and are closely related to strains from Thailand, Japan, and Korea, whereas unique isolates different from other countries were also found in Taiwan. Distinct seasonal distributions were found in different sequence types. Some sequence types caused disease in the cold season, whereas others caused disease in the warm season.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0608","type":"journal-article","created":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T13:27:59Z","timestamp":1283347679000},"page":"658-663","source":"Crossref","is-referenced-by-count":40,"title":["Phylogenetic Analysis of 56-kDa Type-Specific Antigen Gene of Orientia tsutsugamushi Isolates in Taiwan"],"prefix":"10.4269","volume":"83","author":[{"given":"Hsiu-Ying","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-Hsien","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Kai","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Hsin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jr-Shiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chien-Ling","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huai-Chin","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsi-Chieh","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyh-Hsiung","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei-Yun","family":"Shu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Int J Syst Bacteriol","first-page":"589","article-title":"Classification of Rickettsia tsutsugamushi in a new genus, Orientia gen. nov., as Orientia tsutsugamushi comb. nov","volume":"45","author":"Tamura","year":"1995"},{"key":"R2","series-title":"Ann Intern Med","first-page":"26","article-title":"Scrub typhus in South Vietnam. A study of 87 cases","volume":"79","author":"Berman","year":"1973"},{"key":"R3","series-title":"Mil Med","first-page":"269","article-title":"Scrub typhus in Korea: importance of early clinical diagnosis in this newly recognized endemic area","volume":"158","author":"Yi","year":"1993"},{"key":"R4","series-title":"Clin Infect Dis","first-page":"624","article-title":"Life-threatening scrub typhus in a traveler returning from Thailand","volume":"18","author":"Watt","year":"1994"},{"key":"R5","series-title":"Travel Med Infect Dis","first-page":"35","article-title":"Rickettsial diseases in travelers","volume":"1","author":"Walker","year":"2003"},{"key":"R6","series-title":"Curr Opin Infect Dis","first-page":"429","article-title":"Scrub typhus and tropical rickettsioses","volume":"16","author":"Watt","year":"2003"},{"key":"R7","series-title":"Infect Immun","first-page":"603","article-title":"Identification and partial characterization of Rickettsia tsutsugamushi major protein immunogens","volume":"50","author":"Hanson","year":"1985"},{"key":"R8","series-title":"Infect Immun","first-page":"671","article-title":"Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting","volume":"48","author":"Tamura","year":"1985"},{"key":"R9","series-title":"Infect Immun","first-page":"1427","article-title":"Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi","volume":"57","author":"Ohashi","year":"1989"},{"key":"R10","series-title":"Vaccine","first-page":"2","article-title":"Neutralization epitopes on the antigenic domain II of the Orientia tsutsugamushi 56-kDa protein revealed by monoclonal antibodies","volume":"19","author":"Seong","year":"2000"},{"key":"R11","series-title":"FEMS Microbiol Lett","first-page":"163","article-title":"Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes","volume":"180","author":"Enatsu","year":"1999"},{"key":"R12","series-title":"Microbiol Immunol","first-page":"577","article-title":"Phylogenetic characterization of Orientia tsutsugamushi isolated in Taiwan according to the sequence homologies of 56-kDa type-specific antigen genes","volume":"47","author":"Qiang","year":"2003"},{"key":"R13","series-title":"Am J Trop Med Hyg","first-page":"299","article-title":"Short report: variation in the 56-kD type-specific antigen gene of Orientia tsutsugamushi isolated from patients in Thailand","volume":"68","author":"Kollars","year":"2003"},{"key":"R14","series-title":"FEMS Immunol Med Microbiol","first-page":"335","article-title":"Genetic typing of the 56-kDa type-specific antigen gene of contemporary Orientia tsutsugamushi isolates causing human scrub typhus at two sites in north-eastern and western Thailand","volume":"52","author":"Blacksell","year":"2008"},{"key":"R15","series-title":"Am J Trop Med Hyg","first-page":"256","article-title":"Isolation and identification of a novel spotted fever group Rickettsia, strain IG-1, from Ixodes granulatus ticks collected on Orchid Island (Lanyu), Taiwan","volume":"79","author":"Tsai","year":"2008"},{"key":"R16","series-title":"Emerg Infect Dis","first-page":"1970","article-title":"Human case of Rickettsia felis infection, Taiwan","volume":"14","author":"Tsai","year":"2008"},{"key":"R17","series-title":"Mol Biol Evol","first-page":"1596","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007"},{"key":"R18","series-title":"PHYLIP (Phylogeny Inference Package) version 3.6","author":"Felsenstein","year":"2005"},{"issue":"(Suppl 3)","key":"R19","series-title":"Clin Infect Dis","first-page":"S203","article-title":"Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi.","volume":"15","author":"Kelly","year":"2009"},{"key":"R20","series-title":"J Parasitol","first-page":"653","article-title":"Correlation of chigger abundance with temperature at a hyperendemic focus of scrub typhus","volume":"62","author":"Van Peenen","year":"1976"},{"key":"R21","series-title":"Formosan Entomol","first-page":"257","article-title":"Studies on the vectors and pathogens of scrub typhus on murine-like animals in Kinmen County, Taiwan","volume":"24","author":"Wang","year":"2004"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/3\/article-p658.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/3\/article-p658.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/3\/article-p658.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T12:07:15Z","timestamp":1725278835000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/3\/article-p658.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9]]},"references-count":21,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0608","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9]]}}}