{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T16:04:05Z","timestamp":1776960245053,"version":"3.51.4"},"reference-count":34,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6]]},"abstract":"Four major malaria-causing Plasmodium<\/jats:italic> spp. and lymphatic filariasis-causing Wuchereria bancrofti<\/jats:italic> are co-endemic in many tropical and sub-tropical regions. Among molecular diagnostic assays, multiplex polymerase chain reaction (PCR)\u2013based assays for the simultaneous detection of DNAs from these parasite species are currently available only for P. falciparum<\/jats:italic> and W. bancrofti<\/jats:italic> or P. vivax<\/jats:italic> and W. bancrofti<\/jats:italic>. Using a post-PCR oligonucleotide ligation detection reaction\u2013fluorescent microsphere assay (LDR-FMA), we developed a multiplex assay that has the capability to simultaneously detect all four Plasmodium<\/jats:italic> spp. and W. bancrofti<\/jats:italic> infections in blood samples. Compared with microfilarial positivity in the blood, the LDR-FMA assay is highly concordant (91%), sensitive (86%), and specific (94%), and has high reproducibility for Plasmodium<\/jats:italic> spp. (85\u201393%) and W. bancrofti<\/jats:italic> (90%) diagnoses. The development of this assay for the simultaneous diagnosis of multiple parasitic infections enables efficient screening of large numbers of human blood and mosquito samples from co-endemic areas.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0665","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T19:04:52Z","timestamp":1275505492000},"page":"1030-1033","source":"Crossref","is-referenced-by-count":16,"title":["Molecular-Based Assay for Simultaneous Detection of Four Plasmodium spp. and Wuchereria bancrofti Infections"],"prefix":"10.4269","volume":"82","author":[{"given":"Rajeev K.","family":"Mehlotra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie R.","family":"Gray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melinda 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