{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T02:59:53Z","timestamp":1793501993351,"version":"4.5.0"},"reference-count":28,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>Here, we determined the <jats:italic>Toxoplasma gondii<\/jats:italic> genotype in amniotic fluid, placenta, and cerebrospinal fluid samples from 14 congenital toxoplasmosis cases in Tunisia, North Africa. Direct genotypic characterization of <jats:italic>T. gondii<\/jats:italic> strains was performed by polymerase chain reaction (PCR) amplification of six genetic markers (3'<jats:italic>SAG2<\/jats:italic>, 5' <jats:italic>SAG2<\/jats:italic>, <jats:italic>SAG3<\/jats:italic>, <jats:italic>BTUB<\/jats:italic>, <jats:italic>GRA6<\/jats:italic>, and <jats:italic>APICO<\/jats:italic>) and thereafter, was analyzed by restriction fragment-length polymorphism (RFLP). Samples were sequenced to resolve strain type whenever there were unclear enzyme digestion results. Multilocus analysis revealed that only one specimen harbored the type I allele in all studied loci, whereas the 13 others gave mixed genotype results with different alleles at different markers. Seven specimens produced RFLP profile of the recombinant strains I\/III, and three produced a profile of I\/II recombinant strains. The last three specimens produced complex digestion patterns. In these cases, sequence analysis revealed double peaks at known polymorphic sites, indicating the presence of multiple alleles.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0691","type":"journal-article","created":{"date-parts":[[2010,6,2]],"date-time":"2010-06-02T19:04:52Z","timestamp":1275505492000},"page":"1041-1046","source":"Crossref","is-referenced-by-count":48,"title":["Direct Genotypic Characterization of Toxoplasma gondii Strains Associated with Congenital Toxoplasmosis in Tunisia (North Africa)"],"prefix":"10.4269","volume":"82","author":[{"given":"Sonia","family":"Boughattas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rym","family":"Ben-Abdallah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emna","family":"Siala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olfa","family":"Souissi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00efda","family":"Bouratbine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Med Mal Infect","first-page":"139","article-title":"Mode de contamination, incidence et prevalence de la 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