{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:36:02Z","timestamp":1791344162272,"version":"4.3.1"},"reference-count":34,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,10,5]]},"abstract":"<jats:p>A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0697","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T17:15:08Z","timestamp":1285953308000},"page":"760-765","source":"Crossref","is-referenced-by-count":77,"title":["Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans"],"prefix":"10.4269","volume":"83","author":[{"given":"Ximena","family":"Collao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Palacios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"de Ory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Sanbonmatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"P\u00e9rez-Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Molina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen K.","family":"Hutchison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian W.","family":"Lipkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Tenorio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paz","family":"S\u00e1nchez-Seco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses","first-page":"695","author":"Nichol","year":"2005"},{"key":"R2","series-title":"Trans R Soc Trop Med Hyg","first-page":"109","article-title":"Impact of climate change and other factors on emerging arbovirus diseases","volume":"103","author":"Gould","year":"2009"},{"key":"R3","series-title":"J Med Virol","first-page":"86","article-title":"Detection of neurotropic viruses circulating in Tuscany: the incisive role of Toscana virus","volume":"60","author":"Valassina","year":"2000"},{"key":"R4","series-title":"Arch Virol Suppl","first-page":"41","article-title":"Sandfly fever viruses in Italy","volume":"11","author":"Nicoletti","year":"1996"},{"key":"R5","series-title":"Clin Infect Dis","first-page":"1241","article-title":"Encephalitis without meningitis due to sandfly fever virus serotype toscana","volume":"32","author":"Dionisio","year":"2001"},{"key":"R6","series-title":"Scand J Infect Dis","first-page":"505","article-title":"Phlebotomus-transmitted toscana virus infections of the central nervous system: a seven-year experience in Tuscany","volume":"30","author":"Braito","year":"1998"},{"key":"R7","series-title":"J Pediatr","first-page":"144","article-title":"Toscana virus infections of the central nervous system in children: a report of 14 cases","volume":"132","author":"Braito","year":"1998"},{"key":"R8","series-title":"Eur J Intern Med","first-page":"316","article-title":"Sandfly fever due to toscana virus: an emerging infection in southern France","volume":"15","author":"Hemmersbach-Miller","year":"2004"},{"key":"R9","series-title":"Lancet","first-page":"1212","article-title":"Neurovirulent toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal","volume":"1","author":"Ehrnst","year":"1985"},{"key":"R10","series-title":"Clin Infect Dis","first-page":"434","article-title":"Infections due to sandfly fever virus serotype toscana in Spain","volume":"27","author":"Mendoza-Montero","year":"1998"},{"key":"R11","series-title":"Am J Trop Med Hyg","first-page":"429","article-title":"Central nervous system involvement during infection by phlebovirus toscana of residents in natural foci in central Italy (1977\u20131988)","volume":"45","author":"Nicoletti","year":"1991"},{"key":"R12","series-title":"Vector Borne Zoonotic Dis","first-page":"519","article-title":"Massilia virus, a novel phlebovirus (Bunyaviridae) isolated from sandflies in the Mediterranean","volume":"9","author":"Charrel","year":"2009"},{"key":"R13","series-title":"Vector Borne Zoonotic Dis","first-page":"203","article-title":"Surveillance of arboviruses in Spanish wetlands: detection of new flavi- and phleboviruses","volume":"10","author":"Sanchez-Seco","year":"2010"},{"key":"R14","series-title":"Emerg Infect Dis","first-page":"1701","article-title":"Toscana virus in Spain","volume":"11","author":"Sanbonmatsu-Gamez","year":"2005"},{"key":"R15","series-title":"J Med Virol","first-page":"140","article-title":"Detection and identification of toscana and other phleboviruses by RT-nested-PCR assays with degenerated primers","volume":"71","author":"Sanchez-Seco","year":"2003"},{"key":"R16","series-title":"Mol Biol Evol","first-page":"1596","article-title":"MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007"},{"key":"R17","series-title":"Nucleic Acids Res","first-page":"4876","article-title":"The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools","volume":"25","author":"Thompson","year":"1997"},{"key":"R18","series-title":"Mol Biol Evol","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993"},{"key":"R19","series-title":"J Clin Microbiol","first-page":"3170","article-title":"Diagnostic potential of toscana virus N protein expressed in Escherichia coli","volume":"36","author":"Valassina","year":"1998"},{"key":"R20","series-title":"J Clin Microbiol","first-page":"1519","article-title":"Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India","volume":"44","author":"Domingo","year":"2006"},{"key":"R21","series-title":"J Clin Microbiol","first-page":"6176","article-title":"Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region","volume":"43","author":"Casas","year":"2005"},{"key":"R22","series-title":"J Clin Microbiol","first-page":"1869","article-title":"Molecular identification of mumps virus genotypes from clinical samples: standardized method of analysis","volume":"43","author":"Palacios","year":"2005"},{"key":"R23","series-title":"J Virol","first-page":"8196","article-title":"Genetic reassortment of Rift Valley fever virus in nature","volume":"73","author":"Sall","year":"1999"},{"key":"R24","series-title":"Vaccine","first-page":"369","article-title":"Use of reassortant viruses to map attenuating and temperature-sensitive mutations of the Rift Valley fever virus MP-12 vaccine","volume":"8","author":"Saluzzo","year":"1990"},{"key":"R25","series-title":"J Gen Virol","first-page":"2307","article-title":"Generation and transmission of Rift Valley fever viral reassortants by the mosquito Culex pipiens","volume":"71","author":"Turell","year":"1990"},{"key":"R26","series-title":"Arch Virol","first-page":"2237","article-title":"Natural M-segment reassortment in Potosi and Main Drain viruses: implications for the evolution of orthobunyaviruses","volume":"152","author":"Briese","year":"2007"},{"key":"R27","series-title":"J Virol","first-page":"5627","article-title":"Batai and Ngari viruses: M segment reassortment and association with severe febrile disease outbreaks in East Africa","volume":"80","author":"Briese","year":"2006"},{"key":"R28","series-title":"J Virol","first-page":"263","article-title":"Use of bacterial expression cloning to define the amino acid sequences of antigenic determinants on the G2 glycoprotein of Rift Valley fever virus","volume":"58","author":"Keegan","year":"1986"},{"key":"R29","series-title":"Am J Trop Med Hyg","first-page":"617","article-title":"Genetic variation among geographic isolates of Rift Valley fever virus","volume":"39","author":"Battles","year":"1988"},{"key":"R30","series-title":"Virology","first-page":"442","article-title":"Antigenic analysis of Punta Toro virus and identification of protective determinants with monoclonal antibodies","volume":"167","author":"Pifat","year":"1988"},{"key":"R31","series-title":"Arch Virol","first-page":"111","article-title":"Topological mapping of antigenic sites on the Rift Valley fever virus envelope glycoproteins using monoclonal antibodies","volume":"121","author":"Besselaar","year":"1991"},{"key":"R32","series-title":"J Virol","first-page":"3162","article-title":"Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants","volume":"64","author":"Schmaljohn","year":"1990"},{"key":"R33","series-title":"Virus Res","first-page":"89","article-title":"Single amino acid substitutions in Puumala virus envelope glycoproteins G1 and G2 eliminate important neutralization epitopes","volume":"48","author":"Horling","year":"1997"},{"key":"R34","series-title":"Emerg Infect Dis","first-page":"574","article-title":"Genetic diversity of toscana virus","volume":"15","author":"Collao","year":"2009"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/4\/article-p760.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/4\/article-p760.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/4\/article-p760.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T16:09:45Z","timestamp":1725293385000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/4\/article-p760.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10,5]]},"references-count":34,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0697","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10,5]]}}}