{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:10:54Z","timestamp":1777648254761,"version":"3.51.4"},"reference-count":66,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,6]]},"abstract":"<jats:p><jats:italic>Leishmania infantum<\/jats:italic> causes visceral leishmaniasis, a severe zoonotic and systemic disease that is fatal if left untreated. Identification of the antigens involved in <jats:italic>Leishmania<\/jats:italic>-specific protective immune response is a research priority for the development of effective control measures. For this purpose, we evaluated, in 27 dogs from an enzootic zone, specific humoral and cellular immune response by delayed-type hypersensitivity (DTH) skin test both against total <jats:italic>L. infantum<\/jats:italic> antigen and the raw <jats:italic>Trichoplusia ni<\/jats:italic> insect-derived kinetoplastid membrane protein-11 (rKMPII), tryparedoxin peroxidase (rTRYP), <jats:italic>Leishmania<\/jats:italic> homologue of receptors for activated C kinase (rLACK), and 22-kDa potentially aggravating protein of <jats:italic>Leishmania<\/jats:italic> (rpapLe22) antigens from this parasite. rTRYP induced the highest number of positive DTH responses (55% of leishmanin skin test [LST]-positive dogs), showing that TRYP antigen is an important T cell immunogen, and it could be a promising vaccine candidate against this disease. When TRYP-DTH and KMPII-DTH tests were evaluated in parallel, 82% of LST-positive dogs were detected, suggesting that both antigens could be considered as components of a standardized DTH immunodiagnostic tool for dogs.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0784","type":"journal-article","created":{"date-parts":[[2010,11,30]],"date-time":"2010-11-30T15:18:22Z","timestamp":1291130302000},"page":"1287-1294","source":"Crossref","is-referenced-by-count":8,"title":["Humoral and In Vivo Cellular Immunity against the Raw Insect-Derived Recombinant Leishmania infantum Antigens KMPII, TRYP, LACK, and papLe22 in Dogs from an Endemic Area"],"prefix":"10.4269","volume":"83","author":[{"given":"Felicitat","family":"Todol\u00ed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laia","family":"Solano-Gallego","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"de Juan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pere","family":"Morell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria del Carmen","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Lasa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"G\u00f3mez-Sebasti\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Escribano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Alberola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alhel\u00ed","family":"Rodr\u00edguez-Cort\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Parasitol Today","first-page":"188","article-title":"The strange case of Leishmania chagasi","volume":"16","author":"Mauricio","year":"2000"},{"key":"R2","series-title":"Trends Parasitol","first-page":"399","article-title":"Canine leishmaniasis: epidemiological risk and the experimental model","volume":"18","author":"Moreno","year":"2002"},{"key":"R3","series-title":"Am J Trop Med Hyg","first-page":"273","article-title":"Canine leishmaniasis: identification of asymptomatic carriers by polymerase chain reaction and immunoblotting","volume":"55","author":"Berrahal","year":"1996"},{"key":"R4","series-title":"J Clin Microbiol","first-page":"560","article-title":"Prevalence of Leishmania infantum infection in dogs living in an area of canine leishmaniasis endemicity using PCR on several tissues and serology","volume":"39","author":"Solano-Gallego","year":"2001"},{"key":"R5","series-title":"Med Vet Entomol","first-page":"339","article-title":"Leishmania infantum infection rates in Phlebotomus perniciosus fed on naturally infected dogs under antimonial treatment","volume":"1","author":"Gradoni","year":"1987"},{"key":"R6","series-title":"Vaccine","first-page":"3277","article-title":"Long lasting protection against canine kala-azar using the FML-QuilA saponin vaccine in an endemic area of Brazil (Sao Goncalo do Amarante, RN)","volume":"20","author":"Borja-Cabrera","year":"2002"},{"key":"R7","series-title":"J Infect Dis","first-page":"535","article-title":"Immunologic markers of clinical evolution in children recently infected with Leishmania donovani chagasi","volume":"165","author":"Carvalho","year":"1992"},{"key":"R8","series-title":"Infect Immun","first-page":"229","article-title":"Cellular and humoral immune responses in dogs experimentally and naturally infected with Leishmania infantum","volume":"62","author":"Pinelli","year":"1994"},{"key":"R9","series-title":"Parasite Immunol","first-page":"531","article-title":"Evidence of subjects sensitized to Leishmania infantum on the French Mediterranean coast: differences in gamma interferon production between this population and visceral leishmaniasis patients","volume":"13","author":"Meller-Melloul","year":"1991"},{"issue":"(Suppl)","key":"R10","series-title":"Parasitology","first-page":"S19","article-title":"Phenotypical characteristics, biochemical pathways, molecular targets and putative role of nitric oxide-mediated programmed cell death in Leishmania","volume":"132","author":"Holzmuller","year":"2006"},{"key":"R11","series-title":"Trans R Soc Trop Med Hyg","first-page":"380","article-title":"Studies in leishmanifasis in East Africa. 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