{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:52:58Z","timestamp":1790671978930,"version":"4.1.0"},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,5]]},"abstract":"<jats:p>The diagnostic value of enzyme-linked immunosorbent assay (ELISA) was evaluated when blood specimens of 92 patients suspected of brucellosis underwent the ELISA (IgM and IgG), standard tube agglutination (SAT), and 2-mercaptoethanol (2-ME) tests and blood cultures; 38 sera from non-brucellosis patients and 34 sera from blood donors were also subjected to ELISA, SAT, and 2<jats:bold>-<\/jats:bold>ME tests. SAT was able to pinpoint only 23 (25%), whereas ELISA confirmed the etiology in 56 (60.9%; <jats:italic>P<\/jats:italic> &lt; 0.001) patients with brucellosis, including 31 culture-confirmed cases. The sensitivity and specificity of ELISA were 100% and 71.31%, respectively. Because they were confirmed by ELISA, the diagnosis could never be excluded with SAT in 33 cases. ELISA has been found to be more sensitive in acute (28% higher sensitivity; <jats:italic>P<\/jats:italic> &lt; 0.02) and chronic (55% higher sensitivity; <jats:italic>P<\/jats:italic> &lt; 0.01) cases. For accurate diagnosis in suspected brucellosis cases detection, we recommend both ELISA IgM and IgG tests. ELISA IgG and 2<jats:bold>-<\/jats:bold>ME tests seem to be promising tools in judging prognosis.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0790","type":"journal-article","created":{"date-parts":[[2010,8,3]],"date-time":"2010-08-03T19:18:56Z","timestamp":1280863136000},"page":"314-318","source":"Crossref","is-referenced-by-count":36,"title":["ELISA versus Conventional Methods of Diagnosing Endemic Brucellosis"],"prefix":"10.4269","volume":"83","author":[{"given":"Basappa","family":"Mantur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisha","family":"Parande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Amarnath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giridhar","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravindra","family":"Walvekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Desai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahantesh","family":"Parande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupali","family":"Shinde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masiyappa","family":"Chandrashekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Emerg Infect Dis","first-page":"485","article-title":"Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp","volume":"9","author":"Sohn","year":"2003"},{"key":"R2","series-title":"J Clin Microbiol","first-page":"4363","article-title":"Characterization of a Brucella spp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand","volume":"44","author":"McDonald","year":"2006"},{"key":"R3","series-title":"FEMS Immunol Med Microbiol","first-page":"49","article-title":"Urban outbreak of a Brucella melitensis infection in an Argentine family: clinical and diagnostic aspects","volume":"8","author":"Wallach","year":"1994"},{"key":"R4","series-title":"Rev Infect Dis","first-page":"821","article-title":"Human brucellosis","volume":"5","author":"Young","year":"1983"},{"key":"R5","series-title":"Am J Trop Med Hyg","first-page":"698","article-title":"Evaluation of brucellosis by PCR and persistence after treatment in patients returning to the hospital for follow-up","volume":"76","author":"Maas","year":"2007"},{"key":"R6","series-title":"J Clin Microbiol","first-page":"691","article-title":"2-mercaptoethanol Brucella agglutination test: usefulness for predicting recovery from brucellosis","volume":"11","author":"Buchanan","year":"1980"},{"key":"R7","series-title":"J Med Microbiol","first-page":"897","article-title":"Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years' experience in an endemic area","volume":"55","author":"Mantur","year":"2006"},{"key":"R8","series-title":"Int J Epidemiol","first-page":"335","article-title":"Sampling survey on brucellosis among farmers and their families in IIIe-et-Vilaine (Brittany)","volume":"6","author":"Zourbas","year":"1977"},{"key":"R9","series-title":"Med J Aust","first-page":"482","article-title":"An epidemiological survey of human brucellosis in three Victorian abattoirs","volume":"1","author":"Gilbert","year":"1980"},{"key":"R10","series-title":"Bull World Health Organ","first-page":"417","article-title":"Comparison of counter-immuno-electrophoresis with other serological tests in the diagnosis of human brucellosis","volume":"53","author":"Daz","year":"1976"},{"key":"R11","series-title":"J Infect","first-page":"197","article-title":"Evaluation of a Brucella enzyme immunoassay test (ELISA) in comparison with bacteriological culture and agglutination","volume":"36","author":"Gad El-Rab","year":"1998"},{"issue":"(Suppl 1)","key":"R12","series-title":"J Chemother","first-page":"54","article-title":"Diagnostic value of Brucella ELISA IgG and IgM in bacteremic and non-bacteremic patients with brucellosis","volume":"13","author":"Osoba","year":"2001"},{"key":"R13","series-title":"Medicine (Baltimore)","first-page":"415","article-title":"Brucellosis in the United States, 1960\u20131972: an abattoir-associated disease. Part II. Diagnostic aspects","volume":"53","author":"Buchanan","year":"1974"},{"key":"R14","series-title":"Clin Infect Dis","first-page":"131","article-title":"Specific antibody profile in human brucellosis","volume":"14","author":"Ariza","year":"1992"},{"key":"R15","series-title":"J Infect Dis","first-page":"219","article-title":"Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up","volume":"159","author":"Gazapo","year":"1989"},{"key":"R16","series-title":"N Engl J Med","first-page":"1263","article-title":"Significance of 7S and macroglobulin Brucella agglutinins in human brucellosis","volume":"272","author":"Reddin","year":"1965"},{"key":"R17","series-title":"Lancet","first-page":"19","article-title":"Diagnosis of human brucellosis with ELISA","volume":"2","author":"Sippel","year":"1982"},{"key":"R18","series-title":"J Med Microbiol","first-page":"167","article-title":"An enzyme-labelled immunosorbent assay for Brucella abortus antibodies","volume":"13","author":"Magee","year":"1980"},{"key":"R19","series-title":"Rev Infect Dis","first-page":"359","article-title":"Serologic diagnosis of human brucellosis: analysis of 214 cases by agglutination tests and review of the literature","volume":"13","author":"Young","year":"1991"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/2\/article-p314.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/2\/article-p314.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/2\/article-p314.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T15:59:54Z","timestamp":1725292794000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/2\/article-p314.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,5]]},"references-count":19,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0790","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8,5]]}}}