{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:23:21Z","timestamp":1790601801687,"version":"4.1.0"},"reference-count":37,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>In spite of the high prevalence of malaria in Southeastern Bangladesh, there remains a significant shortage of information regarding the presence of three of five human malaria parasites: <jats:italic>Plasmodium ovale<\/jats:italic>, <jats:italic>P. malariae<\/jats:italic>, and <jats:italic>P. knowlesi<\/jats:italic>. The presence of <jats:italic>P. ovale<\/jats:italic> and <jats:italic>P. knowlesi<\/jats:italic> has previously never been reported from Bangladesh. We used a genus- and species-specific nested polymerase chain reaction, targeting highly conserved regions of the small subunit ribosomal RNA (SSU rRNA) gene, to investigate the presence of malaria parasites in a total number of 379 patient samples in a survey of patients with febrile illnesses in the Chittagong Hill Tracts in Southeastern Bangladesh. We identified the first cases of <jats:italic>P. ovale<\/jats:italic> in Bangladesh. They were confirmed by sequence analysis; 189 of 379 samples (49.9%; 95% confidence interval = 44.9\u201354.9%) were positive for <jats:italic>Plasmodium<\/jats:italic> sp. by PCR. <jats:italic>P. falciparum<\/jats:italic> monoinfections accounted for 68.3% (61.3\u201374.5%), followed by <jats:italic>P. vivax<\/jats:italic> (15.3%; 10.9\u201321.2%), <jats:italic>P. malariae<\/jats:italic> (1.6%; 0.5\u20134.6%), <jats:italic>P. ovale<\/jats:italic> (1.6%; 0.5\u20134.6%), and mixed infections (13.2%; 9.1\u201318.8%). We found no evidence of <jats:italic>P. knowlesi<\/jats:italic> in this region.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.09-0796","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T16:25:01Z","timestamp":1278001501000},"page":"75-78","source":"Crossref","is-referenced-by-count":34,"title":["Indigenous Plasmodium ovale Malaria in Bangladesh"],"prefix":"10.4269","volume":"83","author":[{"given":"Hans-Peter","family":"Fuehrer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Starzengruber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Swoboda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wasif Ali","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Matt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Ley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamala","family":"Thriemer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashidul","family":"Haque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emran Bin","family":"Yunus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shah Monir","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Walochnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Noedl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"World Malaria Report 2008","author":"World Health Organization","year":"2008"},{"key":"R2","series-title":"N Engl J Med","first-page":"540","article-title":"Artemisinin-resistant malaria in Asia","volume":"361","author":"Noedl","year":"2009"},{"key":"R3","series-title":"N Engl J Med","first-page":"2619","article-title":"Evidence of artemisinin-resistant malaria in western Cambodia","volume":"359","author":"Noedl","year":"2008"},{"key":"R4","series-title":"World Malaria Report","author":"World Health Organization","year":"2005"},{"key":"R5","series-title":"Malar J","first-page":"173","article-title":"Knowledge on the transmission, prevention and treatment of malaria among two endemic populations of Bangladesh and their health-seeking behaviour","volume":"8","author":"Ahmed","year":"2009"},{"key":"R6","series-title":"Malar J","first-page":"185","article-title":"Spatial malaria epidemiology in Bangladeshi highlands","volume":"8","author":"Haque","year":"2009"},{"key":"R7","series-title":"Bangladesh Med Res Counc Bull","first-page":"87","article-title":"Malaria in southeast Bangladesh: a descriptive study","volume":"34","author":"Maude","year":"2008"},{"key":"R8","series-title":"Am J Trop Med Hyg","first-page":"396","article-title":"Failure of national guidelines to diagnose uncomplicated malaria in Bangladesh","volume":"67","author":"Faiz","year":"2002"},{"key":"R9","series-title":"PLoS One","first-page":"e6737","article-title":"Malaria prevalence in endemic districts of Bangladesh","volume":"4","author":"Haque","year":"2009"},{"key":"R10","series-title":"Trop Med Int Health","first-page":"680","article-title":"Drug resistance in Plasmodium falciparum from the Chittagong Hill Tracts, Bangladesh","volume":"9","author":"van den Broek","year":"2004"},{"key":"R11","series-title":"Parasitol Today","first-page":"422","article-title":"How prevalent are Plasmodium ovale and P. malariae in East Asia?","volume":"15","author":"Kawamoto","year":"1999"},{"key":"R12","series-title":"Am J Trop Med Hyg","first-page":"206","article-title":"An indigenous case of Plasmodium ovale infection in Sri Lanka","volume":"78","author":"Wickremasinghe","year":"2008"},{"key":"R13","series-title":"J Vector Borne Dis","first-page":"206","article-title":"Plasmodium ovale\u2014a case report from Gujarat","volume":"43","author":"Marathe","year":"2006"},{"key":"R14","series-title":"Trop Med Int Health","first-page":"231","article-title":"Wide distribution of Plasmodium ovale in Myanmar","volume":"7","author":"Win","year":"2002"},{"key":"R15","series-title":"Trop Med Int Health","first-page":"304","article-title":"High prevalence of Plasmodium malariae and Plasmodium ovale in malaria patients along the Thai-Myanmar border, as revealed by acridine orange staining and PCR-based diagnosis","volume":"3","author":"Zhou","year":"1998"},{"key":"R16","series-title":"Am J Trop Med Hyg","first-page":"719","article-title":"Large sequence heterogeneity of the small subunit ribosomal RNA gene of Plasmodium ovale in Cambodia","volume":"72","author":"Incardona","year":"2005"},{"key":"R17","series-title":"Clin Infect Dis","first-page":"172","article-title":"Plasmodium knowlesi: the fifth human malaria parasite","volume":"46","author":"White","year":"2008"},{"key":"R18","series-title":"Lancet","first-page":"1017","article-title":"A large focus of naturally acquired Plasmodium knowlesi infections in human beings","volume":"363","author":"Singh","year":"2004"},{"key":"R19","series-title":"Clin Infect Dis","first-page":"165","article-title":"Plasmodium knowlesi malaria is widely distributed and potentially life threatening","volume":"46","author":"Cox-Singh","year":"2008"},{"key":"R20","series-title":"Int J Parasitol","first-page":"1125","article-title":"Plasmodium knowlesi from archival blood films: further evidence that human infections are widely distributed and not newly emergent in Malaysian Borneo","volume":"39","author":"Lee","year":"2009"},{"key":"R21","series-title":"Emerg Infect Dis","first-page":"811","article-title":"Human infections with Plasmodium knowlesi, the Philippines","volume":"14","author":"Luchavez","year":"2008"},{"key":"R22","series-title":"J Infect Dis","first-page":"1143","article-title":"Differential prevalence of Plasmodium infections and cryptic Plasmodium knowlesi malaria in humans in Thailand","volume":"199","author":"Putaporntip","year":"2009"},{"key":"R23","series-title":"Emerg Infect Dis","first-page":"814","article-title":"Naturally acquired human Plasmodium knowlesi infection, Singapore","volume":"14","author":"Ng","year":"2008"},{"key":"R24","series-title":"Emerg Infect Dis","first-page":"2211","article-title":"Naturally acquired Plasmodium knowlesi malaria in human, Thailand","volume":"10","author":"Jongwutiwes","year":"2004"},{"key":"R25","series-title":"Malar J","first-page":"73","article-title":"Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections","volume":"8","author":"Lee","year":"2009"},{"key":"R26","series-title":"Status of South Asian Primates. Conservation Assessment and Management Plan (CAMP). Workshop Report 2003","first-page":"127","author":"Molur","year":"2003"},{"key":"R27","series-title":"Trends Parasitol","first-page":"406","article-title":"Knowlesi malaria: newly emergent and of public health importance?","volume":"24","author":"Cox-Singh","year":"2008"},{"key":"R28","series-title":"Med Vet Entomol","first-page":"158","article-title":"Six new species of the Anopheles leucosphyrus group, reinterpretation of An. elegans and vector implications","volume":"19","author":"Sallum","year":"2005"},{"key":"R29","series-title":"Am J Trop Med Hyg","first-page":"249","article-title":"Short report: rapid DNA extraction from archive blood spots on filter paper for genotyping of Plasmodium falciparum","volume":"72","author":"Bereczky","year":"2005"},{"key":"R30","series-title":"Am J Trop Med Hyg","first-page":"42","article-title":"Storage duration and polymerase chain reaction detection of Plasmodium falciparum from blood spots on filter paper","volume":"69","author":"Chaorattanakawee","year":"2003"},{"key":"R31","series-title":"Am J Trop Med Hyg","first-page":"687","article-title":"A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemilogical studies","volume":"60","author":"Singh","year":"1999"},{"key":"R32","series-title":"Methods Mol Med","first-page":"189","article-title":"Nested PCR analysis of Plasmodium parasites","volume":"72","author":"Snounou","year":"2002"},{"key":"R33","series-title":"Trop Med Int Health","first-page":"231","article-title":"Wide distribution of Plasmodium ovale in Myanmar","volume":"7","author":"Win","year":"2002"},{"key":"R34","series-title":"Am J Trop Med Hyg","first-page":"257","article-title":"A field study on malaria prevalence in southeastern Laos by polymerase chain reaction assay","volume":"64","author":"Toma","year":"2001"},{"key":"R35","series-title":"Emerg Infect Dis","first-page":"1434","article-title":"Monkey malaria in a European traveler returning from Malaysia","volume":"14","author":"Kantele","year":"2008"},{"key":"R36","series-title":"Malar J","first-page":"15","article-title":"Swedish traveler with Plasmodium knowlesi malaria after visiting Malaysian Borneo","volume":"8","author":"Bronner","year":"2009"},{"key":"R37","series-title":"Malar J","first-page":"86","article-title":"Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers","volume":"8","author":"Steenkeste","year":"2009"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/1\/article-p75.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/1\/article-p75.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/1\/article-p75.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T16:04:32Z","timestamp":1725293072000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/1\/article-p75.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":37,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0796","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}