{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:40:36Z","timestamp":1792712436567,"version":"4.5.0"},"reference-count":32,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:p>The <jats:italic>Aedes aegypti<\/jats:italic> densovirus (AeDNV) has previously shown potential in mosquito control. To improve its efficacy as a biopesticide, the gene for an excitatory insect-specific toxin from <jats:italic>Buthus martensii<\/jats:italic> Karsch (BmK IT1) was inserted into the AeDNV genome and cloned into pUCA plasmid. The coding sequence for green fluorescent protein was ligated to the C-terminus of the BmK IT1 gene as a screening marker. Recombinant and helper plasmids were cotransfected into C6\/36 cells; wild-type viruses were the controls. The recombinant viruses were identified and quantified by real-time polymerase chain reaction and exposed to <jats:italic>Ae. albopictus<\/jats:italic> larvae for the evaluation of its bioinsecticidal activity. LT<jats:sub>50<\/jats:sub> and LD<jats:sub>50<\/jats:sub> bioassays showed that the recombinant AeDNV had stronger and faster pathogenic effects on <jats:italic>Ae. albopictus<\/jats:italic> than the wild-type virus. This is the first report on the recombinant AeDNA containing the insect-specific toxin, BmK IT1, which may be used to develop a novel type of insecticide.<\/jats:p>","DOI":"10.4269\/ajtmh.2010.10-0074","type":"journal-article","created":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T17:27:59Z","timestamp":1283362079000},"page":"614-623","source":"Crossref","is-referenced-by-count":28,"title":["A Recombinant AeDNA Containing the Insect-Specific Toxin, BmK IT1, Displayed an Increasing Pathogenicity on Aedes albopictus"],"prefix":"10.4269","volume":"83","author":[{"given":"Jin-Bao","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Qiao","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Juan","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Guang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Nat Med","first-page":"729","article-title":"Balancing risks on the backs of the poor","volume":"6","author":"Attaran","year":"2000"},{"key":"R2","series-title":"Trends Biotechnol","first-page":"447","article-title":"Genetic manipulation of mosquitoes: an approach to controlling 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