{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T14:13:14Z","timestamp":1789913594449,"version":"4.0.1"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,5]]},"abstract":"<jats:p>Species-specific diagnosis was performed in 66 patients with cutaneous leishmaniasis (CL) living in Tataouine focus in southeastern Tunisia. <jats:italic>Leishmania<\/jats:italic> DNA was extracted directly from dermal scrapings (n = 66) and from parasites obtained in culture (n = 12). Species were identified by using polymerase chain reaction\u2013restriction fragment length polymorphism analysis for internal transcribed spacer region 1 and isoenzyme analysis. <jats:italic>Leishmania tropica<\/jats:italic> and <jats:italic>L. major<\/jats:italic> were identified in 31 (47%) and 35 (53%) cases respectively. <jats:italic>Leishmania tropica<\/jats:italic> CL cases were geographically scattered, and <jats:italic>L. major<\/jats:italic> CL cases were clustered. Lesions caused by <jats:italic>L. tropica<\/jats:italic> were mostly single (83.8%) and face-localized (55.8%), and lesions caused by <jats:italic>L. major<\/jats:italic> were multiple (57.1%; <jats:italic>P<\/jats:italic> &lt; 0.001) and situated on limbs (83.7%; <jats:italic>P<\/jats:italic> &lt; 0.001). For both species, most lesion onsets were reported during June\u2013January. However, lesions that emerged during February\u2013May were mainly caused by <jats:italic>L. tropica<\/jats:italic> (83.3%; <jats:italic>P<\/jats:italic> &lt; 0.01). Moreover, the delay before seeking medical advice was higher for <jats:italic>L. tropica<\/jats:italic> infections than for <jats:italic>L. major<\/jats:italic> infections (<jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p>","DOI":"10.4269\/ajtmh.2010.10-0234","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T11:28:05Z","timestamp":1288351685000},"page":"1034-1039","source":"Crossref","is-referenced-by-count":42,"title":["Epidemiologic and Clinical Features of Cutaneous Leishmaniasis in Southeastern Tunisia"],"prefix":"10.4269","volume":"83","author":[{"given":"Nadia","family":"Bousslimi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Aoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Im\u00e8ne","family":"Ben-Abda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nissaf","family":"Ben-Alaya-Bouafif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Raouane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u00efda","family":"Bouratbine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Leishmania: Taxonomie et Phylogen\u00e8se; Applications \u00c9co-\u00c9pid\u00e9miologiques","first-page":"139","author":"Rioux","year":"1986"},{"key":"R2","series-title":"Trop Med Int Health","first-page":"1071","article-title":"Geographical distribution and epidemiological features of old world cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1,048 strains","volume":"14","author":"Pratlong","year":"2009"},{"key":"R3","series-title":"Bull Soc Pathol Exot","first-page":"323","article-title":"Epid\u00e9miologie de Leishmania (L.) infantum, L. major et L. killicki en Tunisie: r\u00e9sultats et analyse de l'identification de 226 isolats humains et canins et revue de la litt\u00e9rature","volume":"101","author":"Aoun","year":"2008"},{"key":"R4","series-title":"Trans R Soc Trop Med Hyg","first-page":"499","article-title":"Anthroponotic cutaneous leishmaniasis in Tunisia: presence of Leishmania killicki outside its original focus of Tataouine","volume":"99","author":"Haouas","year":"2005"},{"key":"R5","series-title":"Emerg Infect Dis","first-page":"1864","article-title":"Leishmania killicki imported from the Tunisian desert","volume":"15","author":"Maubon","year":"2009"},{"key":"R6","series-title":"Curr Opin Infect Dis","first-page":"97","article-title":"Diagnosis of cutaneous leishmaniasis","volume":"16","author":"Vega-Lopez","year":"2003"},{"key":"R7","series-title":"Diagn Microbiol Infect Dis","first-page":"349","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Schonian","year":"2003"},{"key":"R8","series-title":"Trans R Soc Trop Med Hyg","first-page":"368","article-title":"American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples","volume":"101","author":"Garcia","year":"2007"},{"key":"R9","series-title":"Pathol Biol (Paris)","first-page":"219","article-title":"Les cultures sur milieu NNN dans le diagnostic biologique des leishmanioses","volume":"57","author":"Chouihi","year":"2009"},{"key":"R10","series-title":"Ann Parasitol Hum Comp","first-page":"111","article-title":"Taxonomy of Leishmania. Use of isoenzymes: suggestion for a new classification","volume":"65","author":"Rioux","year":"1990"},{"key":"R11","series-title":"Trans R Soc Trop Med Hyg","first-page":"239","article-title":"The increase in risk factors for leishmaniasis worldwide","volume":"95","author":"Desjeux","year":"2001"},{"key":"R12","series-title":"Epid\u00e9miologie des leishmanioses en Tunisie. Maladies Tropicales Transmissibles","first-page":"73","author":"Ben Ismail","year":"1989"},{"key":"R13","series-title":"Parasite","first-page":"23","article-title":"Phlebotominae (Diptera: Psychodidae) of human leishmaniosis sites in Tunisia","volume":"13","author":"Ghrab","year":"2006"},{"key":"R14","series-title":"Acta Trop","first-page":"131","article-title":"Differences in transmission seasons as an epidemiological tool for characterization of anthroponotic and zoonotic cutaneous leishmaniasis in northern Afghanistan","volume":"105","author":"Faulde","year":"2008"},{"key":"R15","series-title":"Ann Dermatol Venereol","first-page":"1255","article-title":"Formes clinico-\u00e9pid\u00e9miologiques de la leishmaniose cutan\u00e9e en Tunisie","volume":"115","author":"Chaffai","year":"1998"},{"key":"R16","series-title":"Leishmania: Taxonomie et Phylogen\u00e8se; Applications \u00c9co-\u00c9pid\u00e9miologiques","first-page":"221","author":"Bryceson","year":"1986"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/5\/article-p1034.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/5\/article-p1034.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/83\/5\/article-p1034.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T16:25:36Z","timestamp":1725294336000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/83\/5\/article-p1034.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,5]]},"references-count":16,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2010.10-0234","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,5]]}}}