{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:29:55Z","timestamp":1784309395586,"version":"3.55.0"},"reference-count":32,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,2,4]]},"abstract":"<jats:p>This study was conducted to determine which genotypes were present in southwestern Korea. Nested polymerase chain reaction (PCR) and DNA sequence analysis targeting the <jats:italic>Orientia tsutsugamushi<\/jats:italic>-specific 56-kDa protein gene was performed with samples of blood and eschar. Of the 69 PCR-positive samples, 61 clustered with the Boryong previously isolated in Korea. CUH 4-6 had sequence homology of 100% with Kato, and CUH 4-3 had homology of 99.8% with Kato and formed the Kato cluster. CUH 4-57, CUH 4-31, CUH 4-142, and CUH 4-324 formed a Kawasaki cluster. CUH 4-271 had sequence homology of 100% with Jecheon and formed a Karp cluster. CUH 4-117 had homology of 99.8% with Neimeng-65, and Gilliam cluster. The most common genotype of <jats:italic>O. tsutsugamushi<\/jats:italic> in the southwestern part of Korea is the Boryong genotype. We also identified <jats:italic>O. tsutsugamushi<\/jats:italic> of the Kato, Neimeng-65 and Kawasaki genotypes, which had not been identified before in Korea.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.09-0601","type":"journal-article","created":{"date-parts":[[2011,2,3]],"date-time":"2011-02-03T17:02:07Z","timestamp":1296752527000},"page":"250-254","source":"Crossref","is-referenced-by-count":30,"title":["Phylogenetic Analysis of the 56 kDa Protein Genes of Orientia tsutsugamushi in Southwest Area of Korea"],"prefix":"10.4269","volume":"84","author":[{"given":"Yu-Mi","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Min","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Hyun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Sun","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesh Prasad","family":"Neupane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"J Med Assoc Thai","first-page":"464","article-title":"Etiologies of acute undifferentiated febrile illness in Thailand","volume":"87","author":"Leelarasamee","year":"2004"},{"key":"R2","series-title":"Emerg Infect Dis","first-page":"256","article-title":"Rickettsial infections and fever, Vientiane, Laos","volume":"12","author":"Phongmany","year":"2006"},{"key":"R3","series-title":"Ann Trop Med Parasitol","first-page":"363","article-title":"Causes of acute, undifferentiated, febrile illness in rural Thailand: results of a prospective observational study","volume":"100","author":"Suttinont","year":"2006"},{"key":"R4","series-title":"Infect Immun","first-page":"2076","article-title":"The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope","volume":"58","author":"Stover","year":"1990"},{"key":"R5","series-title":"Infect Immun","first-page":"1156","article-title":"Molecular cloning and expression of Rickettsia tsutsugamushi genes for two major protein antigens in Escherichia coli","volume":"55","author":"Oaks","year":"1987"},{"key":"R6","series-title":"Gene","first-page":"119","article-title":"Cloning and sequencing of the gene (tsg56) encoding a type-specific antigen from Rickettsia tsutsugamushi","volume":"91","author":"Ohashi","year":"1990"},{"key":"R7","series-title":"J Biol Chem","first-page":"12728","article-title":"Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants","volume":"267","author":"Ohashi","year":"1992"},{"key":"R8","series-title":"J Vet Med Sci","first-page":"749","article-title":"Two-step polymerase chain reaction for diagnosis of scrub typhus and identification of antigenic variants of Rickettsia tsutsugamushi","volume":"55","author":"Kawamori","year":"1993"},{"key":"R9","series-title":"Infect Immun","first-page":"1427","article-title":"Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi","volume":"57","author":"Ohashi","year":"1989"},{"key":"R10","series-title":"Am J Trop Med Hyg","first-page":"528","article-title":"Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction","volume":"65","author":"Ree","year":"2001"},{"key":"R11","series-title":"Am J Trop Med Hyg","first-page":"218","article-title":"The influence of chemotherapy on the mortality rates of tsutsugamushi disease in northern Japan, and some other statistical information","volume":"5","author":"Hara","year":"1956"},{"key":"R12","series-title":"Am J Trop Med Hyg","first-page":"542","article-title":"Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: a prospective study","volume":"75","author":"Kim","year":"2006"},{"key":"R13","series-title":"J Clin Microbiol","first-page":"1637","article-title":"Serotype-specific amplification of Rickettsiaa tsutsugamushi DNA by nested polymerase chain reaction","volume":"31","author":"Furuya","year":"1993"},{"key":"R14","series-title":"FEMS Microbiol Lett","first-page":"163","article-title":"Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes","volume":"180","author":"Enatsu","year":"1999"},{"key":"R15","series-title":"Microbiol Immunol","first-page":"439","article-title":"Epidemiological survey of Orientia tsutsugamushi distribution in field rodents in Saitama Prefecture, Japan, and discovery of a new type","volume":"45","author":"Tamura","year":"2001"},{"key":"R16","series-title":"Microbiol Immunol","first-page":"201","article-title":"Isolation of Orientia tsutsugamushi from Leptotrombidium fuji and its characterization","volume":"44","author":"Tamura","year":"2000"},{"key":"R17","series-title":"Microbiol Immunol","first-page":"577","article-title":"Phylogenetic characterization of Orientia tsutsugamushi isolated in Taiwan according to the sequence homologies of 56-kDa type-specific antigen genes","volume":"47","author":"Qiang","year":"2003"},{"key":"R18","series-title":"Proc Natl Acad Sci USA","first-page":"7981","article-title":"The Orientia tsutsugamushi genome reveals massive proliferation of conjugative type IV secretion system and host-cell interaction genes","volume":"104","author":"Cho","year":"2007"},{"key":"R19","series-title":"FEMS Microbiol Lett","first-page":"37","article-title":"T-track PCR fingerprinting for the rapid detection of genetic polymorphism","volume":"152","author":"Seong","year":"1997"},{"key":"R20","series-title":"J Hyg Epidemiol Microbiol Immunol","first-page":"18","article-title":"Antigenic diversity of Rickettsia tsutsugamushi: epidemiologic and ecologic significance","volume":"12","author":"Elisberg","year":"1968"},{"key":"R21","series-title":"Clin Microbiol Infect","first-page":"168","article-title":"Detection of new genotypes of Orientia tsutsugamushi infecting humans in Thailand","volume":"14","author":"Fournier","year":"2008"},{"key":"R22","series-title":"J Med Entomol","first-page":"237","article-title":"The ecology of chigger-borne rickettsiosis (scrub typhus)","volume":"11","author":"Traub","year":"1974"},{"key":"R23","series-title":"Microbiol Immunol","first-page":"873","article-title":"Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp, and Gilliam strains from patients","volume":"28","author":"Tamura","year":"1984"},{"key":"R24","series-title":"Microbiol Immunol","first-page":"611","article-title":"Immunological properties of Rickettsia tsutsugamushi, Kawasaki strain, isolated from a patient in Kyushu","volume":"30","author":"Yamamoto","year":"1986"},{"key":"R25","series-title":"Jpn J Med Sci Biol","first-page":"255","article-title":"Serologic analysis of scrub typhus isolates from the Pescadores and Philippine Islands","volume":"35","author":"Shirai","year":"1986"},{"key":"R26","series-title":"J Bacteriol Virol","first-page":"219","article-title":"Seroepidemiological survey of scrub typhus in Korea, 1995\u20131996","volume":"32","author":"Choi","year":"1997"},{"key":"R27","series-title":"Diagn Microbiol Infect Dis","first-page":"185","article-title":"Determination and geographic distribution of Orientia tsutsugamushi serotypes in Thailand by nested polymerase chain reaction","volume":"55","author":"Manosroi","year":"2006"},{"key":"R28","series-title":"Emerg Infect Dis","first-page":"1483","article-title":"Genotyping of Orientia tsutsugamushi from humans with scrub typhus, Laos","volume":"14","author":"Parola","year":"2008"},{"key":"R29","series-title":"FEMS Microbiol Lett","first-page":"299","article-title":"Phylogenetic position of Rickettsia tsutsugamushi and the relationship among its antigenic variants by analyses of 16S rRNA gene sequences","volume":"125","author":"Ohashi","year":"1995"},{"key":"R30","series-title":"Microbiol Immunol","first-page":"67","article-title":"Sequence analysis of the hypervariable regions of the 56 kDa immunodominant protein genes of Orientia tsutsugamushi strains in Malaysia","volume":"49","author":"Tay","year":"2005"},{"key":"R31","series-title":"J Clin Microbiol","first-page":"685","article-title":"Serological classification by monoclonal antibodies of Rickettsia tsutsugamushi isolated in Korea","volume":"28","author":"Chang","year":"1990"},{"key":"R32","series-title":"J Clin Microbiol","first-page":"598","article-title":"High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays","volume":"31","author":"Kim","year":"1993"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/2\/article-p250.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/2\/article-p250.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/2\/article-p250.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T20:04:54Z","timestamp":1723061094000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/2\/article-p250.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,4]]},"references-count":32,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2011.09-0601","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,4]]}}}