{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:27:48Z","timestamp":1762079268458},"reference-count":27,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,5,5]]},"abstract":"<jats:p>Peridomestic transmission of American cutaneous leishmaniasis is increasingly reported and dogs may be a reservoir of <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) in this setting. We investigated the prevalence of infection in dogs in Chaparral County, Colombia, the focus of an epidemic of human cutaneous leishmaniasis caused by <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) <jats:italic>guyanensis<\/jats:italic>. Two (0.72%) of 279 dogs had lesions typical of cutaneous leishmaniasis that were biopsy positive by kinetoplast DNA polymerase chain reaction\u2013Southern blotting. Seroprevalence was 2.2% (6 of 279) by enzyme-linked immunosorbent assay. Buffy coat and ear skin biopsy specimens were positive by polymerase chain reaction\u2013Southern blotting in 7.3% (10 of 137) and 11.4% (12 of 105) of dogs, respectively. Overall 20% of dogs (21 of 105) showed positive results for one or more tests. Amplification and sequencing of the <jats:italic>Leishmania<\/jats:italic> 7SL RNA gene identified <jats:italic>L. guyanensis<\/jats:italic> in one dog and <jats:italic>L. braziliensis<\/jats:italic> in two dogs. No association was identified between the risk factors evaluated and canine infection. Dogs may contribute to transmission but their role in this focus appears to be limited.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.10-0159","type":"journal-article","created":{"date-parts":[[2011,5,3]],"date-time":"2011-05-03T18:53:58Z","timestamp":1304448838000},"page":"674-680","source":"Crossref","is-referenced-by-count":30,"title":["Leishmania (Viannia) Infection in the Domestic Dog in Chaparral, Colombia"],"prefix":"10.4269","volume":"84","author":[{"given":"Juli\u00e1n","family":"Santaella","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara B.","family":"Ocampo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy G.","family":"Saravia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabi\u00e1n","family":"M\u00e9ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"G\u00f3ngora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Adelaida","family":"Gomez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard E.","family":"Munstermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupert J.","family":"Quinnell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Lancet","first-page":"1191","article-title":"Leishmaniasis","volume":"354","author":"Herwaldt","year":"1999"},{"author":"Estad\u00edsticas de la Vigilancia en Salud P\u00fablica","key":"R2"},{"key":"R3","series-title":"Inf Quinc Epidemiol Nac","first-page":"40","article-title":"Informe de leishmaniasis, Colombia, Semanas 1 a 52 de 2005","volume":"11","author":"Zambrano","year":"2006"},{"key":"R4","series-title":"Am J Trop Med Hyg","first-page":"276","article-title":"Etiologic agent of an epidemic of cutaneous leishmaniasis in Tolima, Colombia","volume":"78","author":"Rodriguez-Barraquer","year":"2008"},{"issue":"(Suppl 1):","key":"R5","series-title":"Biomedica","first-page":"249","article-title":"Leishmania (Viannia) braziliensis infection in two Colombian dogs: a note on infectivity for sand flies and response to treatment","volume":"26","author":"Travi","year":"2006"},{"key":"R6","series-title":"Trans R Soc Trop Med Hyg","first-page":"15","article-title":"Natural infections of Leishmania peruviana in animals in the Peruvian Andes","volume":"93","author":"Llanos-Cuentas","year":"1999"},{"key":"R7","series-title":"Am J Trop Med Hyg","first-page":"530","article-title":"Is the domestic dog (Canis familiaris) a reservoir host of American cutaneous leishmaniasis? A critical review of the current evidence","volume":"61","author":"Reithinger","year":"1999"},{"key":"R8","series-title":"Exp Parasitol","first-page":"13","article-title":"Leishmania (Viannia) braziliensis: epidemiology of canine cutaneous leishmaniasis in the State of Parana (Brazil)","volume":"117","author":"Castro","year":"2007"},{"key":"R9","series-title":"J Clin Microbiol","first-page":"1486","article-title":"Evaluation of PCR as a diagnostic mass-screening tool to detect Leishmania (Viannia) spp. in domestic dogs (Canis familiaris)","volume":"41","author":"Reithinger","year":"2003"},{"key":"R10","series-title":"Am J Trop Med Hyg","first-page":"243","article-title":"Environmental risk factors for the incidence of American cutaneous leishmaniasis in a sub-Andean zone of Colombia","volume":"82","author":"Valderrama-Ardila","year":"2010"},{"key":"R11","series-title":"Am J Trop Med Hyg","first-page":"86","article-title":"Epidemiologic, genetic, and clinical associations among phenotypically distinct populations of Leishmania (Viannia) in Colombia","volume":"59","author":"Saravia","year":"1998"},{"key":"R12","series-title":"Am J Trop Med Hyg","first-page":"296","article-title":"A general classification of New World Leishmania using numerical zymotaxonomy","volume":"50","author":"Cupolillo","year":"1994"},{"key":"R13","series-title":"Parasitology","first-page":"143","article-title":"The epidemiology of canine leishmaniasis: transmission rates estimated from a cohort study in Amazonian Brazil","volume":"115","author":"Quinnell","year":"1997"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"423","article-title":"Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction","volume":"72","author":"Vergel","year":"2005"},{"key":"R15","series-title":"J Infect Dis","first-page":"638","article-title":"Detection of Leishmania in unaffected mucosal tissues of patients with cutaneous leishmaniasis caused by Leishmania (Viannia)","volume":"200","author":"Figueroa","year":"2009"},{"key":"R16","series-title":"Current Protocols in Molecular Biology","first-page":"6.2.1","author":"Ausubel","year":"1987"},{"key":"R17","series-title":"Diagn Microbiol Infect Dis","first-page":"432","article-title":"An enhanced method for the identification of Leishmania spp. using real-time polymerase chain reaction and sequence analysis of the 7SL RNA gene region","volume":"66","author":"Stevenson","year":"2010"},{"key":"R18","series-title":"Am J Trop Med Hyg","first-page":"206","article-title":"Distribution and etiology of leishmaniasis in Colombia","volume":"42","author":"Corredor","year":"1990"},{"key":"R19","series-title":"Mem Inst Oswaldo Cruz","first-page":"581","article-title":"Cutaneous leishmaniasis in Venezuela caused by infection with a new hybrid between Leishmania (Viannia) braziliensis and L. (V.) guyanensis","volume":"92","author":"Delgado","year":"1997"},{"key":"R20","series-title":"Vet Parasitol","first-page":"1","article-title":"Canine infection and the possible role of dogs in the transmission of American tegumentary leishmaniosis in Salta, Argentina","volume":"110","author":"Padilla","year":"2002"},{"key":"R21","series-title":"Am J Trop Med Hyg","first-page":"473","article-title":"The transmission dynamics of canine American cutaneous leishmaniasis in Huanuco, Peru","volume":"69","author":"Reithinger","year":"2003"},{"issue":"(Suppl 1):","key":"R22","series-title":"Biomedica","first-page":"95","article-title":"Lutzomyia longiflocosa, posible vector en un foco de leishmaniasis cut\u00e1nea en la regi\u00f3n subandina del departamento del Tolima, Colombia, y el conocimiento que tiene la poblaci\u00f3n sobre este insecto","volume":"26","author":"Pardo","year":"2006"},{"key":"R23","series-title":"Trans R Soc Trop Med Hyg","first-page":"141","article-title":"Domestic dog ownership: a risk factor for human infection with Leishmania (Viannia) species","volume":"97","author":"Reithinger","year":"2003"},{"key":"R24","series-title":"Parasitology","first-page":"1915","article-title":"Transmission, reservoir hosts and control of zoonotic visceral leishmaniasis","volume":"136","author":"Quinnell","year":"2009"},{"key":"R25","series-title":"Trans R Soc Trop Med Hyg","first-page":"611","article-title":"The importance of edentates (sloths and anteaters) as primary reservoirs of Leishmania braziliensis guyanensis, causative agent of \u201cpianbois\u201d in north Brazil","volume":"75","author":"Lainson","year":"1981"},{"key":"R26","series-title":"Mem Inst Oswaldo Cruz","first-page":"125","article-title":"Infeccao experimental de Lutzomyia whitmani em caes infectados com Leishmania braziliensis braziliensis","volume":"81","author":"Vexenat","year":"1986"},{"issue":"(Suppl 1):","key":"R27","series-title":"Biomedica","first-page":"242","article-title":"Dog (Canis familiaris) infectivity to Lutzomyia youngi in Trujillo, Venezuela","volume":"26","author":"Hernandez","year":"2006"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/5\/article-p674.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/5\/article-p674.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/84\/5\/article-p674.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:55:58Z","timestamp":1723060558000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/84\/5\/article-p674.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5,5]]},"references-count":27,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.2011.10-0159","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2011,5,5]]}}}