{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:55:51Z","timestamp":1791395751806,"version":"4.3.3"},"reference-count":35,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,2,4]]},"abstract":"<jats:p>We tested 513 stool samples from patients in Sydney, Australia for <jats:italic>Blastocystis<\/jats:italic> by using five diagnostic techniques: microscopy of a permanently stained smear using a modified iron-hematoxylin stain, two xenic culture systems (a modified Boeck and Drbohlav's medium and tryptone, yeast extract, glucose, methionine-9 medium), and two published conventional polymerase chain reaction methods specific for the small subunit ribosomal DNA. Ninety-eight (19%) samples were positive for <jats:italic>Blastocystis<\/jats:italic> in one or more of the diagnostic techniques. The PCR 2 method was the most sensitive at detecting <jats:italic>Blastocystis<\/jats:italic> with a sensitivity of 94%, and the least sensitive was microscopy of the permanent stain (48%). Subtype 3 was the most predominant subtype (present in 43% of samples assigned to this group). This study highlights the low sensitivity of microscopy when used as the sole diagnostic modality for detection of <jats:italic>Blastocystis<\/jats:italic> sp.<\/jats:p>","DOI":"10.4269\/ajtmh.2011.10-0447","type":"journal-article","created":{"date-parts":[[2011,2,3]],"date-time":"2011-02-03T17:02:07Z","timestamp":1296752527000},"page":"308-312","source":"Crossref","is-referenced-by-count":103,"title":["Comparison of Microscopy, Culture, and Conventional Polymerase Chain Reaction for Detection of Blastocystis sp. in Clinical Stool Samples"],"prefix":"10.4269","volume":"84","author":[{"given":"Tamalee","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Barratt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Harkness","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Stark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Microbiol Rev","first-page":"563","article-title":"Blastocystis hominis revisited","volume":"9","author":"Stenzel","year":"1996"},{"key":"R2","series-title":"Int J Parasitol","first-page":"789","article-title":"Recent advances in Blastocystis 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